Active Placenta Growth Factor (PLGF) Mus musculus (Mouse) Active protein

PlGF2; PGF; PGFL; Placental Growth Factor-Like; Vascular Endothelial Growth Factor-Related Protein

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Overview
Properties
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point7.4
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  • Active Placenta Growth Factor (PLGF) Packages (Simulation)
  • Active Placenta Growth Factor (PLGF) Packages (Simulation)
  • APA114Mu61.jpg SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

A114Mu is a research-grade monoclonal antibody specifically targeting Placenta Growth Factor (PLGF). Monoclonal antibodies like A114Mu are laboratory-engineered proteins designed to bind to a single, precise epitope on their target molecule—here, PLGF. Its development stems from the need to study PLGF’s biological roles, particularly in angiogenesis (blood vessel formation) and pathological conditions such as cancer and preeclampsia. A114Mu enables researchers to block PLGF activity in vitro and in vivo, helping elucidate its interactions with receptors like VEGFR-1. This tool is critical for validating PLGF as a therapeutic target, as its inhibition may suppress abnormal blood vessel growth in tumors or regulate placental vascular development in pregnancy-related disorders.To detect the activity of recombinant PLGF, a functional ELISA assay was performed to evaluate the interaction between recombinant human PLGF and recombinant human NRP1.Briefly, biotin-linked PLGF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100µl were then transferred to NRP1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of PLGF and NRP1 was shown in Figure 1, the EC50 for this effect is 1.12355µg/mL.

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Mutant hypoxia inducible factor-1α improves angiogenesis and tissue perfusion in ischemic rabbit skeletal muscle PubMed: 20937289
  • Elevated PLGF contributes to small-cell lung cancer brain metastasisPubMed: 22797069
  • Photoreceptor avascular privilege is shielded by soluble VEGF receptor-1PubMed: PMC3687373
  • Sustained dual release of placental growth factor-2 and bone morphogenic protein-2 from heparin-based nanocomplexes for direct osteogenesisPubmed:27042064
  • Serum angiogenic profile in abnormal placentationPubmed:26863111
  • Administration of atosiban in patients with endometriosis undergoing frozen–thawed embryo transfer: a prospective, randomized studyPubmed:27143518
  • Placental Growth Factor Triggers Epithelial-to-Mesenchymal Transition-like Changes in Rat Type II Alveolar Epithelial Cells: Activation of Nuclear Factor κB Signalling Pathwaydoi:10.1111
  • Placenta growth factor contributes to cell apoptosis and epithelial-to-mesenchymal transition in the hyperoxia-induced acute lung injuryPubmed:27211521
  • Knockdown of placental growth factor (PLGF) mitigates hyperoxia-induced acute lung injury in neonatal rats: Suppressive effects on NFκB signaling pathwayPubmed:27280587
  • Relationship between elevated serum level of placental growth factor and status of gestational diabetes mellitusPubmed: 30935303
  • sFlt-1/PlGF Ratio in Prediction of Short-Term Neonatal Outcome of Small for Gestational Age Neonates34438609
  • Puerarin Reduces Radiation-Induced Vascular Endothelial Cell Damage Via miR-34a/Placental Growth FactorPubmed:35110976

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