Active Plasminogen Activator, Urokinase Receptor (uPAR) Rattus norvegicus (Rat) Active protein

CD87; PLAUR; PLAU-R; U-PAR; URKR; Monocyte Activation Antigen Mo3

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point6.4
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  • Active Plasminogen Activator, Urokinase Receptor (uPAR) Packages (Simulation)
  • Active Plasminogen Activator, Urokinase Receptor (uPAR) Packages (Simulation)
  • APA141Ra01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Plasminogen Activator, Urokinase Receptor (uPAR) is the receptor for urokinase plasminogen activator and, given its role in localizing and promoting plasmin formation, likely influences many normal and pathological processes related to cell-surface plasminogen activation and localized degradation of the extracellular matrix. The urokinase receptor (uPAR) was identified as the membrane receptor of the serine protease urokinase (uPA), thereby implicated in the plasminogen activation cascade and regulation of pericellular proteolysis, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat uPAR and recombinant human uPA. Briefly, biotin-linked uPAR were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to uPA-coated microtiter wells and incubated for 1h at 37 ℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 ℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of uPAR and uPA was shown in Figure 1, the EC50 for this effect is 0.223 ug/mL.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Dietary supplementation with methylseleninic acid, but not selenomethionine, reduces spontaneous metastasis of Lewis lung carcinoma in miceWiley: source
  • Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with AutismPubMed: PMC3623607
  • Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD)PubMed: PMC3762604
  • Clinical value of soluble urokinase-type plasminogen activator receptor in the diagnosis, prognosis, and therapeutic guidance of sepsisPubMed: 26615223
  • Evaluation of Diagnostic Value of Soluble Urokinase-Type Plasminogen Activator Receptor in SepsisArchcid: 26346
  • Clinical value of soluble urokinase-type plasminogen activator receptor in the diagnosis, prognosis, and therapeutic Cavia (Guinea pig )idance of sepsisscience:S0735675715009559
  • Evaluation of Plasma Urokinase-Type Plasminogen Activator Receptor (UPAR) in Patients With Chronic Hepatitis B, C and Non-Alcoholic Fatty Liver Disease (NAFLD) …10.1016:j.jceh.2018.02.001
  • The diagnostic value of serum urokinase-type plasminogen activator receptor in acute appendicitisPubmed: 31475329
  • Predictive Value of Degranulating Factors of Neutrophils in Massive Cerebral Infarction33787356

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