Active Platelet Factor 4 (PF4) Homo sapiens (Human) Active protein

CXCL4; SCYB4; Chemokine C-X-C-Motif Ligand 4; Oncostatin-A; Iroplact

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Overview
Properties
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 97%
  • Isoelectric Point6.5
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  • Active Platelet Factor 4 (PF4) Packages (Simulation)
  • Active Platelet Factor 4 (PF4) Packages (Simulation)
  • Active Platelet Factor 4 (PF4) Figure . Gene Sequencing (extract)
  • APA172Hu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). This chemokine is released from alpha-granules of activated platelets during platelet aggregation, and promotes blood coagulation by moderating the effects of heparin-like molecules. Due to these roles, it is predicted to play a role in wound repair and inflammation. It was reported that PF4 can inhibits U937 cells proliferation. To test the effect of U937 on cell apoptosis, U937 cells were seeded into 96-well plates at a density of 5,000 cells/well with 5% serum standard 1640 including various concentrations of recombinant Human PF4. After incubated for 48 h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 4 hours at 37℃. Proliferation of U937 cells after incubation with PF4 for 48 h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 assay after incubation with recombinant Human PF4 for 48 h. The result was shown in Figure 2. It was obvious that PF4 significantly inhibit cell viability of U937 cells. The ED50 is 2.2-3.1μg/mL.

Usage

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Propofol lowers serum PF4 level and partially corrects hypercoagulopathy in endotoxemic ratsPubMed: 20601223
  • Secretome of apoptotic peripheral blood cells (APOSEC) attenuates microvascular obstruction in a porcine closed chest reperfused acute myocardial infarction model: role of platelet aggregation and vasodilationPubMed: 22899170
  • Platelets Recognize Brain-Specific Glycolipid Structures, Respond to Neurovascular Damage and Promote NeuroinflammationPubMed: PMC3608633
  • Effect of splenectomy on platelet activation and decompression sickness outcome in a rat model of decompressionPubmed:25311322
  • A Phellinus baumii–based supplement containing Salvia miltiorrhiza Bunge improves atherothrombotic profiles through endothelial nitric oxide synthase and cyclooxygenase pathwaysin vitro and in vivoscience:S1756464616300676
  • Aspirin, but Not Tirofiban Displays Protective Effects in Endotoxin Induced Lung Injurypubmed:27583400
  • Evidence of the Role of R-Spondin 1 and Its Receptor Lgr4 in the Transmission of Mechanical Stimuli to Biological Signals for Bone Formation.pubmed:28272338
  • Feasibility of improving platelet‑rich plasma therapy by using chitosan with high platelet activation abilitypubmed:28450960
  • Aspirin pre-treatment modulates ozone-induced fetal growth restriction and alterations in uterine blood flow in ratsPubmed: 30528429
  • Short and long-term changes in platelet and inflammatory biomarkers after cryoballoon and radiofrequency ablationPubmed: 30857843
  • Evaluating the Platelet Activation Related to the Degradation of Biomaterials by Scheme of Molecular Markers
  • Newly Identified HNP‐F from Human Neutrophil Peptide 1 Promotes HemostasisPubmed: 30927490
  • Data for short and long-term prothrombotic biomarkers after cryoballoon and radiofrequency ablation
  • Evaluating Platelet Activation Related to the Degradation of Biomaterials Using Molecular MarkersPubmed: 32812629
  • The Inhibitory Effect of Protamine on Platelets is Attenuated by Heparin without Inducing Thrombocytopenia in RodentsPubmed: 31533230
  • Feasibility study of use of rabbit blood to evaluate platelet activation by medical devicesPubmed: 31838449
  • The C5a/C5a receptor 1 axis controls tissue neovascularization through CXCL4 release from platelets34099640
  • The effect of ChAdOx1 nCov-19 vaccine on arterial thrombosis development and platelet aggregation in female ratsPubmed:35183388

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