Active Protein Kinase, AMP Activated Alpha 1 (AMPK Alpha 1) Homo sapiens (Human) Active protein

PRKAa1; AMPK; AMPKa1; PKA; ACACA kinase; 5'-AMP-Activated Protein Kinase Catalytic Subunit Alpha-1; Acetyl-CoA carboxylase kinase; Hydroxymethylglutaryl-CoA reductase kinase

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Overview
Properties
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point8.5
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  • Active Protein Kinase, AMP Activated Alpha 1 (AMPK Alpha 1) Packages (Simulation)
  • Active Protein Kinase, AMP Activated Alpha 1 (AMPK Alpha 1) Packages (Simulation)
  • APA679Hu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

AMPK alpha 1, also known as AMP-activated protein kinase alpha 1 subunit, is a crucial enzyme that plays a significant role in energy metabolism. It is is involved in regulating several metabolic processes, including glucose and fatty acid uptake, oxidation, and storage. It promotes energy-producing pathways, such as glycolysis, fatty acid oxidation, and mitochondrial biogenesis, while inhibiting energy-consuming processes, like protein synthesis and lipogenesis. Besides, PRKAb1 has been identified as an interactor of AMPK Alpha 1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human AMPK Alpha 1 and recombinant human PRKAb1. Briefly, AMPK Alpha 1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to PRKAb1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-AMPK Alpha 1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human AMPK Alpha 1 and recombinant human PRKAb1 was shown in Figure 1, the EC50 for this effect is 0.94 ug/mL.

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Deletion of the serine protease CAP2/Tmprss4 leads to dysregulated renal water handling upon dietary potassium depletionPubmed: 31863073

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