Active Protein Kinase B Alpha (PKBa) Homo sapiens (Human) Active protein

AKT1; AKT; PKB-A; Proto-oncogene c-Akt; RAC-Alpha Serine/Threonine-Protein Kinase; V-akt Murine Thymoma Viral Oncogene Homolog 1

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point6.5
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  • Active Protein Kinase B Alpha (PKBa) Packages (Simulation)
  • Active Protein Kinase B Alpha (PKBa) Packages (Simulation)
  • APC231Hu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Protein Kinase B Alpha (PKBa), also known as AKT1, is a key member of the AKT family of serine/threonine kinases, which play a crucial role in various cellular processes including cell growth, proliferation, differentiation, and survival. PKBa is activated by insulin and growth factor signaling and phosphorylates a variety of substrates to regulate these cellular functions.The activation of akt1 requires the association with Heat Shock Protein 90kDa Alpha B1 (HSP90aB1), resulting in an active auto-regulatory cycle.Thus a functional ELISA assay was conducted to detect the interaction of recombinant human PKBa and recombinant human HSP90aB1. Briefly, PKBa was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to HSP90aB1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-PKBa pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human PKBa and recombinant human HSP90aB1 was shown in Figure 1, the EC50 for this effect is 0.149ug/mL.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Profiling of selected angiogenesis-related genes in proliferative eutopic endometrium of women with endometriosisPubmed: 24188612
  • Beta-arrestin 1 is involved in the catabolic response stimulated by hyaluronan degradation in mouse chondrocytesPubmed:25673209
  • Encapsulated VEGF 121-PLA microparticles promote angiogenesis in human endometrium stromal cells33523322
  • In Vivo and In Vitro Enhanced Tumoricidal Effects of Metformin, Active Vitamin D3, and 5-Fluorouracil Triple Therapy against Colon Cancer by Modulating the …Pubmed:35326689
  • Metformin and Calcitriol Enhance 5-Fluorouracil Tumoricidal Effects in Colon Cancer By Modulating The PI3K/Akt/PTEN/mTOR Network

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