Active Renin (REN) Sus scrofa; Porcine (Pig) Active protein

angiotensinogenase

No quotes Distributors
Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point6.4
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • Active Renin (REN) Packages (Simulation)
  • Active Renin (REN) Packages (Simulation)
  • APA889Po61.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Renin (REN) is an enzyme protein. It is mainly secreted by the juxtaglomerular cells of the kidney. Its main function is to cleave angiotensinogen in the blood to produce angiotensin I, thereby initiating the renin-angiotensin-aldosterone system (RAAS), which plays a crucial role in regulating blood pressure and maintaining fluid and electrolyte balance in the body. Besides,Endothelin 1 (EDN1) has been identified as an interactor of REN, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant pig REN and recombinant rat EDN1.Briefly, biotin-linked REN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to EDN1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of REN and EDN1 was shown in Figure 1, the EC50 for this effect is 0.41ug/mL.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Prenatal overexposure to glucocorticoids programs renal 11β-hydroxysteroid dehydrogenase type 2 expression and salt-sensitive hypertension in the ratPubMed: 21045727
  • Recombinant human erythropoietin in humans down-regulates proximal renal tubular reabsorption and causes a fall in glomerular filtration rate Physoc: 194241
  • Erythropoietin down‐regulates proximal renal tubular reabsorption and causes a fall in glomerular filtration rate in humansPubMed: 20724370
  • Anti-Angiotensin and Hypoglycemic Treatments Suppress Liver Metastasis of Colon Cancer CellsKarger: 000330169
  • The Angiotensin Receptor Blocker, Azilsartan Medoxomil (TAK-491), Suppresses Vascular Wall Expression of Plasminogen Activator Inhibitor Type-I Protein Potentially Facilitating the Stabilization of Atherosclerotic PlaquesLWW: 08000
  • Multiple and large simple renal cysts are associated with prehypertension and hypertension.Pubmed: 23389415
  • Estimation of the parameters of the renin-angiotensin-aldosterone system, water-electrolyte metabolism, and endothelial function in newborns of women with chronic hypertensionSpringer:Source
  • Organ-Specific Activation of the Gastric Branch of the Efferent Vagus Nerve by Ghrelin in Urethane-Anesthetized RatsPubmed:24366191
  • VSL#3 probiotic treatment decreases bacterial translocation in rats with carbon tetrachloride-induced cirrhosisPubmed:24750552
  • Immunological and hemostasiological disorders in women with ovarian hyperstimulation syndromePubmed:25200828
  • Age-Associated Changes in the Vascular Renin-Angiotensin System in MicePubmed:27200147
  • Ablation of Potassium-Chloride Cotransporter Type 3 (Kcc3) in Mouse Causes Multiple Cardiovascular Defects and Isosmotic PolyuriaPubmed:27166674
  • Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated withActivation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension.pubmed:27391973
  • Efficacy and Safety of Renal Sympathetic Denervation on Dogs with Pressure Overload-Induced Heart Failure.pubmed:27555054
  • Sacubitril/valsartan (LCZ696) Significantly Reduces Aldosterone and Increases cGMP Circulating Levels in a Canine Model of RAAS ActivationDoi: 10.1101/435560
  • Gut Dysbiosis Contribute to High Fructose Induced Salt Sensitive Hypertension In Sprague-Dawley Rats
  • The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice33303990
  • Association of Ang-(1¨C7) and des-Arg 9 BK as new biomarkers of obesity and cardiometabolic risk factors in adolescents33568792
  • Phosphorylation of β1-integrin in juxtaglomerular cells helps control blood pressure during the progression of diabetic nephropathy
  • Bedeutung des intrarenalen Renin-Angiotensin-Systems f¨¹r die Entwicklung der chronischen Nierenkrankheit

Recommend products