Active S100 Calcium Binding Protein A7 (S100A7) Mus musculus (Mouse) Active protein

S100-A7; PSOR1; S100A7c; Psoriasin 1

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point5.7
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  • Active S100 Calcium Binding Protein A7 (S100A7) Packages (Simulation)
  • Active S100 Calcium Binding Protein A7 (S100A7) Packages (Simulation)
  • APC035Mu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

S100 Calcium Binding Protein A7 (S100A7) is a calcium - binding protein predominantly expressed in epithelial cells. It plays roles in innate immunity, inflammation, and cell growth regulation. Linked to psoriasis and cancer, S100A7 exhibits antibacterial activity.S100A7 interacts with S100A11, forming heterodimers that may enhance their functions in cellular signaling and inflammation - related processes.Thus a functional ELISA assay was conducted to detect the interaction of recombinant mouse S100A7 and recombinant human S100A11. Briefly, S100A7 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to S100A11-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-S100A7 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse S100A7 and recombinant human S100A11 was shown in Figure 1, the EC50 for this effect is 0.066ug/mL.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Serum levels of antimicrobial peptides and proteins do not correlate with psoriasis severity and are increased after treatment with fumaric acid estersSpringerLink: t07u24t43r357l15
  • Proteomic Signature of Endothelial Dysfunction Identified in the Serum of Acute Ischemic Stroke Patients by the iTRAQ-Based LC–MS ApproachPubMed: 25807139
  • S100A7 (psoriasin) inhibits human epidermal differentiation by enhanced IL-6 secretion through IκB/NF-κB signalingPubmed:27060579
  • S100A7 (psoriasin) inhibits human epidermal differentiation by enhanced IL‐6 secretion through IκB/NF‐κB signallingpubmed:27060579
  • Plasma proteins as potential targets of abnormal Savda syndrome in asthma patients treated with unique Uighur prescriptionpubmed:28672924
  • The psoriasis-associated IL-17A induces and cooperates with IL-36 cytokines to control keratinocyte differentiation and function.pubmed:29142248
  • A potential contribution of psoriasin to vascular and epithelial abnormalities and inflammation in systemic sclerosisPubmed:28681537
  • Measurements of AMPs in stratum corneum of atopic dermatitis and healthy skin–tape stripping techniquePubmed:29374283
  • Comprehensive proteomic profiling of patients' tears identifies potential biomarkers for the traumatic vegetative statePubmed:30019218
  • Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detectionPubmed: 31706945
  • Alarmins HMGB1, IL-33, S100A7, and S100A12 in Psoriasis Vulgaris

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