Active S100 Calcium Binding Protein A9 (S100A9) Mus musculus (Mouse) Active protein

MRP14; MIF; 60B8AG; CFAG; NIF; CAGB; CGLB; L1AG; P14; Calgranulin B; Migration inhibitory factor-related protein 14; Calprotectin L1H subunit; Leukocyte L1 complex heavy chain

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 80%
  • Isoelectric Point6.9
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  • Active S100 Calcium Binding Protein A9 (S100A9) Packages (Simulation)
  • Active S100 Calcium Binding Protein A9 (S100A9) Packages (Simulation)
  • APB793Mu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

S100 calcium-binding protein 9 (S100A9) also known as calgranulin B, is a member of the S100 family of secreted calcium binding proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Lactoferrin (LTF) is one of targets of S100A9, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse S100A9 and recombinant human LTF. Briefly, S100A9 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to LTF-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-S100A9 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse S100A9 and recombinant human LTF was shown in Figure 1, the EC50 for this effect is 0.44 ug/mL.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Differential Proteomic Analysis Distinguishes Tissue Repair Biomarker Signatures in Wound Exudates Obtained from Normal Healing and Chronic WoundsPubmed: source
  • High circulating levels of S100A8/A9 complex (calprotectin) in male Japanese with abdominal adiposity and dysregulated expression of S100A8 and S100A9 in adipose tissues of obese miceScienceDirect: S0006291X1200352X
  • Proteins with Altered Levels in Plasma from Glioblastoma Patients as Revealed by iTRAQ-Based Quantitative Proteomic AnalysisPubMed: 23029420
  • Identifizierung neuer potentieller Biomarker für das Kolonkarzinom sowie dessen VorstufenInfo:Source
  • Susceptibility to COPD: Differential Proteomic Profiling after Acute SmokingPubmed:25036363
  • Soluble receptor for advanced glycation end products inhibits disease progression in autosomal dominant polycystic kidney disease by down-regulating cell proliferationPubMed: 25934702
  • S100A9 and ORM1 serve as predictors of therapeutic response and prognostic factors in advanced extranodal NK/T cell lymphoma patients treated with pegaspargase/gemcitabinepmc:PMC4810364
  • Proteomics Analysis of Blood Serums from Alzheimer's Disease Patients Using iTRAQ LabelingTechnology.pubmed:27911324
  • Immune Modulating Topical S100A8/A9 Inhibits Growth of Pseudomonas aeruginosa and Mitigates Biofilm Infection in Chronic Wounds.pubmed:28672877
  • Tu1938-Micronutrients Assessment in Patients with Cirrhosis10.1016:S0016-5085(18)33544-3
  • Tu1942-PI3K/AKT-Mediated Upregulation of C7 Inhibits Colorectal Cancer Proliferation and Metastasis10.1016:S0016-5085(18)33544-5
  • Signaling between pancreatic β-cells and macrophages via S100 calcium-binding protein A8 exacerbates β-cell apoptosis and islet inflammationPubmed:29496993
  • The role of calgranulin B gene on the biological behavior of squamous cervical cancer in vitro and in vivoPubmed:29497331
  • S100A8/A9 promotes MMP-9 expression in the fibroblasts from cardiac rupture after myocardial infarction by inducing macrophages secreting TNFα.Pubmed:29949169
  • Estudio de células inmunosupresoras y biomarcadores circulantes en carcinoma renal
  • Mass Spectrometry Analysis of the Exhaled Breath Condensate and Proposal of Dermcidin and S100A9 as Possible Markers for Lung Cancer PrognosisPubmed: 31115649
  • 降钙素原, 超敏C 反应蛋白, 红细胞沉降率及S100A9 对肺结核合并肺部细菌感染的影响
  • Serum S100A6, S100A8, S100A9 and S100A11 proteins in colorectal neoplasia: results of a single centre prospective studyPubmed: 31856598
  • Adverse reproductive effects of S100A9 on bovine sperm and early embryonic development in vitroPubmed: 31945120
  • Overexpression of S100A9 in tumor stroma contribute to immune evasion of NK/T cell lymphoma and predict poor response rate34045609
  • Adjunctive S100A8/A9 Immunomodulation Hinders Cipro fl oxacin Resistance in

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