Active Serum Amyloid A (SAA) Homo sapiens (Human) Active protein

SA-A; SAA1; PIG4; TP53I4; Amyloid protein A; Amyloid fibril protein AA; Serum amyloid A-1 protein

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Overview
Properties
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point6.3
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  • Active Serum Amyloid A (SAA) Packages (Simulation)
  • Active Serum Amyloid A (SAA) Packages (Simulation)
  • APA885Hu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Serum Amyloid A (SAA) is a class of 104 amino acid conservative acute-phase proteins, which is essential in immune-mediated inflammatory processes, like IBD. SAA has dual roles in the inflammatory process, which it can stimulates the pro-inflammatory cytokine expression and promotes the pathogenic differentiation of TH17 cells. In addition, SAA can remove toxic lipids produced during inflammatory responses and membrane debris from dead cells, redirect HDL, and recycle cholesterol for tissue repair. Besides, C Reactive Protein (CRP) has been identified as an interactor of SAA, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human SAA and recombinant human CRP. Briefly, SAA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CRP-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-SAA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human SAA and recombinant human CRP was shown in Figure 1, the EC50 for this effect is 5.3 ug/mL.

Usage

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Effect of feeding a high fat diet on hydrogen sulfide (H 2 S) metabolism in the mousePubmed:24637018
  • Acute Phase Response: Implication in ST-segment Elevation Myocardial InfarctionPubmed:24894970
  • Comparison of the Effects of Enoxaparin and Heparin on Inflammatory Biomarkers in Patients with ST-segment Elevated Myocardial Infarction: A prospective Open Label Pilot Clinical TrialPubmed:25237354
  • No association between vitamin D levels and inflammation markers in patients with acute coronary syndromePubmed:25615956
  • Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical studyPubmed:25652121
  • EFFECT OF UTERINE IMMUNOMODULATION ON SERUM AMYLOID-A CONCENTRATION AND CONCEPTION RATE IN CYCLIC NON BREEDING COWSTcrjournals:Source
  • Impaired adipogenesis in adipose tissue associated with hepatic lipid deposition induced by chronic inflammation in mice with chew dietPubMed: 26188592
  • Relation of thyroid hormone abnormalities with subclinical inflammatory activity in patients with type 1 and type 2 diabetes mellitusPubMed: 26049370
  • The effect of serum and follicular fluid amyloid-associated protein levels on in vitro fertilization outcome in patients with polycystic ovary syndromePubMed: 26463878
  • Circulating serum amyloid A: a potential biomarker for infection after aneurysmal subarachnoid hemorrhagefiles:ijcem0021201
  • An Anti-β-Amyloid Vaccine for Treating Cognitive Deficits in a Mouse Model of Down SyndromePubmed:27023444
  • PATHWAY SPECIFIC MARKERS FOR DIAGNOSING IRRITABLE BOWEL SYNDROMEy2016:0139148.html
  • Acute phase response in lame crossbred dairy cattlepubmed:27956769
  • A ligand-specific blockade of the integrin Mac-1 selectively targets pathologic inflammation while maintaining protective host-defensePubmed:29410422
  • Influence of yeast culture and feed antibiotics on ruminal fermentation and site and extent of digestion in beef heifers fed high grain rationsPubmed:30060086
  • Using ruminally protected and nonprotected active dried yeast as alternatives to antibiotics in finishing beef steers: growth performance, carcass traits, blood …Pubmed: 30184125
  • Metabolism and immune status during transition period influences the lactation performance in Zebu (Bos indicus () cowsPubmed: 17430930
  • The predictive value of joint detection of serum amyloid protein A, PCT, and Hs-CRP in the diagnosis and efficacy of neonatal septicemia.Pubmed: 31298341
  • Evaluation of infrared thermography compared to rectal temperature to identify illness in early postpartum dairy cows
  • Ruminally protected and unprotected Saccharomyces cerevisiae fermentation products as alternatives to antibiotics in finishing beef steersPubmed: 31410465
  • Effects of vaccination on acute-phase protein response in broiler chickenPubmed: 32191767
  • AA Amyloid Deposition in the Central and Peripheral Nervous Systems in FlamingosPubmed: 32677873
  • Serum amyloid A as an marker of cow֨ s mastitis caused by Streptococcus sp.Pubmed: 32505957
  • Peripheral concentrations of metabolic and inflammatory indicators during transition period and their relationship with postpartum clinical endometritis in dairy …
  • РОЛЬ СЫВОРОТОЧНОГО АМИЛОИДА А И С-РЕАКТИВНОГО БЕЛКА В ПРОГНОЗИРОВАНИИ ПОСЛЕОПЕРАЦИОННЫХ ОСЛОЖНЕНИЙ И …
  • Processing Index of Barley Grain and Dietary Undigested Neutral Detergent Fiber Concentration Affected Chewing Behavior, Ruminal pH and Total Tract Nutrient?¡­33474564
  • Induction of Serum Amyloid A3 in Mouse Mammary Epithelial Cells Stimulated with Lipopolysaccharide and Lipoteichoic Acid34070499
  • Perfluorooctane sulfonate exerts inflammatory bowel disease-like intestinal injury in rats33510972
  • CCS e amiloide A do leite de quartos mam¨¢rios tratados na secagem com plasma rico em plaquetas aut¨®logo, associado ou n?o a antibi¨®tico
  • The Effect of Feed Supplementation with EM Bokashi® Multimicrobial Probiotic Preparation on Selected Parameters of Sow Colostrum and Milk as Indicators …34596883
  • Effects of dietary turmeric (Curcuma longa) on innate and acquired immune responses in broiler chicken34765801
  • Mahanimbine Improved Aging-Related Memory Deficits in Mice through Enhanced Cholinergic Transmission and Suppressed Oxidative Stress, Amyloid Levels, and …Pubmed:35053756
  • A Three-Protein Panel to Support the Diagnosis of Sepsis in ChildrenPubmed:35329889
  • Serum and salivary adipokines in type 2 diabetes–Results of a pilot study in IndiaPubmed:35717896
  • Clinical significance of differential serum-signatures for early prediction of severe dengue among Eastern Indian patientsPubmed:35348620
  • İneklerde Subklinik Mastitis Tanısında Akut Faz Proteinlerinden Amiloid A ve C-Reaktif Protein Düzeylerinin Değerlendirilmesi

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