Active Superoxide Dismutase 2, Mitochondrial (SOD2) Mus musculus (Mouse) Active protein

IPO-B; MNSOD; Mn-SOD

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point7.3
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  • Active Superoxide Dismutase 2, Mitochondrial (SOD2) Packages (Simulation)
  • Active Superoxide Dismutase 2, Mitochondrial (SOD2) Packages (Simulation)
  • APB083Mu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Extracellular superoxide dismutase [Cu-Zn] is an enzyme that in humans is encoded by the SOD3 gene. This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. Acroding to the report, in a weakly alkaline buffer solution (pH=8.2) with N-tris(hydroxymethyl)amino methane-HCL, pyrogallol can occur autoxidation in the air, then SOD can inhibit this reaction. Thus, we use this way to measued the activity of recombinant mouse SOD2.The reaction was performed in adding 15μl 5mmol/L pyrogallol to 900μl 50mmol/L Tris-HCl in 1.5ml cuvette(1.0 cm light path),rapidly mixing at 25 ℃,then read at 325nm(zero the spectrophotometer using 50mmol/L Tris-HCl) , reacod the OD value every 30 second for 6 times. Control the pyrogallol autoxidation rate at 0.70 OD/min. After, adding various concentrations of recombinant of SOD2 to 900μl 50mmol/L Tris-HCl, incubated for 20min at 25 ℃, then adding 15μl 5mmol/L pyrogallol to each tube, rapidly mixing and read at 325nm, reacod the OD value every 30 second for 6 times. Under these conditions, the enzyme amount of 50% per minute inhibition of pyrogallol autooxidation is defined as a unit.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Antioxidant profile of salivary glands in high fat diet-induced insulin resistance ratsPubmed: 24106991
  • Consequences of age on ischemic wound healing in rats: altered antioxidant activity and delayed wound closurePubmed:24443098
  • Determination of Gene Expression and Serum Levels of MnSOD and GPX1 in Colorectal CancerPubmed:25550558
  • Antioxidant profile, carbonyl and lipid oxidation markers in the parotid and submandibular glands of rats in different periods of streptozotocin induced diabetesPubMed: 26143097
  • Impact of morbid obesity and bariatric surgery on antioxidant/oxidant balance of the unstimulated and stimulated human salivaPubMed: 26608886
  • DDAH1 deficiency promotes intracellular oxidative stress and cell apoptosis via a miR-21-dependent pathway in mouse embryonic fibroblasts.Pubmed:26806551
  • Cardiomyocyte dimethylarginine dimethylaminohydrolase1 attenuates left-ventricular remodeling after acute myocardial infarction: involvement in oxidative stress and …Pubmed:29892894
  • Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndromePubmed: 30342783
  • Selected elements of extracellular matrix of the skin in diabetes and insulin resistancePubmed: 31146169
  • A Machine Learning-driven Study Indicates Emodin Improves Cardiac Hypertrophy by Modulation of Mitochondrial SIRT3 SignalingPubmed: 32135248
  • Arachidonic Acid as an Early Indicator of Inflammation during Non-Alcoholic Fatty Liver Disease DevelopmentPubmed: 32751983
  • Attenuation of Oxidative Stress and Inflammatory Response by Chronic Cannabidiol Administration Is Associated with Improved n-6/n-3 PUFA Ratio in the White and?¡­34064937
  • Serum Total SOD Activity and SOD1/2 Concentrations in Predicting All-Cause Mortality in Lung Cancer Patients34832849
  • α-Lipoic acid ameliorates inflammation state and oxidative stress by reducing the content of bioactive lipid derivatives in the left ventricle of rats fed a high-fat dietPubmed:35569738

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