Active Transferrin Receptor (TFR) Homo sapiens (Human) Active protein

CD71; TFR1; P90; TFRC; T9; Trfr; sTfR; Transferrin receptor protein 1, serum form

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point6.8
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  • Active Transferrin Receptor (TFR) Packages (Simulation)
  • Active Transferrin Receptor (TFR) Packages (Simulation)
  • APB171Hu62.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Transferrin Receptor (TFR) , also known as cluster of differentiation 71 (CD71), is a type II transmembrane glycoprotein that plays a crucial role in the transport of iron into the cell. It binds to Transferrin (TRF), a carrier protein that transports iron in the blood, and facilitates the uptake of iron through endocytosis. This process is essential for many biological functions, including DNA synthesis and cell growth.Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human TFR and recombinant chicken TRF. Briefly, biotin-linked TFR were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to TRF-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of TFR and TRF was shown in Figure 1, the EC50 for this effect is 0.068ug/mL.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Survey of anaemia and Helicobacter pylori infection in adolescent girls in Suihua, China and enhancement of iron intervention effects by H. pylori eradicationPubMed: 22004585
  • The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing micePubmed:26757699
  • The Labile Iron Pool in Monocytes Reflects the Activity of the Atherosclerotic Process in Men with Chronic Cardiovascular Diseasepubmed:27782743
  • Increased NGAL level associated with iron store in chronic kidney disease with anemiaPubmed:29909502
  • Systemic iron deficiency does not affect the cardiac iron content and progression of heart failure34139233

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