Active Transforming Growth Factor Beta 1 (TGFb1) Mus musculus (Mouse) Active protein

TGF-B1; CED; DPD1; LAP; Camurati-Engelmann Disease; Latency-associated peptide

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Overview
Properties
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 97%
  • Isoelectric Point8.4
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  • Active Transforming Growth Factor Beta 1 (TGFb1) Packages (Simulation)
  • Active Transforming Growth Factor Beta 1 (TGFb1) Packages (Simulation)
  • Active Transforming Growth Factor Beta 1 (TGFb1) Figure. Gene Sequencing (Extract)
  • APA124Mu01.png Figure. SDS-PAGE
  • Active Transforming Growth Factor Beta 1 (TGFb1) Figure. Western Blot
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Transforming growth factor beta 1 or TGF-β1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, and apoptosis. To test the effect of TGF-β1 on cell apoptosis, A549 cells were seeded into 96-well plates at a density of 5,000 cells/well with 1% serum standard DMEM including various concentrations of recombinant mouseTGF-β1. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 2 hours at 37℃. Proliferation of A549 cells after incubation with TGF-β1 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 assay after incubation with recombinant mouse TGF-β1 for 48h. The result was shown in Figure 2. It was obvious that TGF-β1 significantly inhibit cell viability of A549 cells. The ED50 is 7.1μg/mL. (A) A549 cells cultured in DMEM, stimulated with 12.5μg/mL TGF-β1 for 48h;
(B) Unstimulated A549 cells cultured in DMEM for 48h.
Figure. Inhibition of A549 cells proliferation after stimulated with TGF-β1

Figure. Inhibition of A549 cells proliferation after stimulated with TGF-β1.

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Metformin attenuates cardiac fibrosis by inhibiting the TGFβ1–Smad3 signalling pathwayOxfordJournals: source
  • Evidence for the complementary and synergistic effects of the three-alkaloid combination regimen containing berberine, hypaconitine and skimmianine on the ulcerative colitis rats induced by trinitrobenzene-sulfonic acidScienceDirect: S0188440910001049
  • Chronic angiotensin-(1–7) administration improves vascular remodeling after angioplasty through the regulation of the TGF-β/Smad signaling pathway in rabbitsScienceDirect: S0006291X09017021
  • Incorporating pTGF-β1/calcium phosphate nanoparticles with fibronectin into 3-dimensional collagen/chitosan scaffolds: efficient, sustained gene delivery to stem cells for chondrogenic differentiation.Pubmed: 22314694
  • A Soluble Antigen-Specific Factor from CD4+CD25+ T Cells of OVA Tolerant Mice Inducing OVA-Specific Peripheral Tolerance Independently in vivoMedwell: 2011642650
  • Increased circulating concentrations of PDGF-BB and TGF-β1 in canine generalised demodicosisRevmedvet: Source
  • The Intervention Effect of Rosiglitozone in Ovarian Fibrosis of PCOS RatsBesjournal: Source
  • Biomarkers of Fibroproliferative Healing in Fibrosing Idiopathic Interstitial PneumoniasPubMed: PMC3551240
  • A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic ApproachesPlosone: Source
  • Fibrogenic response of hepatic stellate cells in ovarectomised rats exposed to ketogenic dietPubmed: 23568967
  • EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHIIjfans: Source
  • Multitargeted protective effect of Abacopteris penangiana against carrageenan-induced chronic prostatitis in rats.Pubmed: 24211397
  • Development of a KLD-12 polypeptide/TGF-β1-tissue scaffold promoting the differentiation of esenchymal stem cell into nucleus pulposus-like cells for treatment of intervertebral disc degenerationIjcep:Source
  • Prolactin anterior pituitary expression and circulating levels are reduced in obese and diabetic rats: Role of TGFβ and TNFαPubmed:25715833
  • PAR2, IL4R, TGFβ and TNFα in bronchoalveolar lavage distinguishes extrinsic allergic alveolitis from sarcoidosisPubmed:Pmc4079423
  • Effect of combined treatment with rosuvastatin and protein kinase Cβ2 inhibitor on angiogenesis following myocardial infarction in diabetic ratsPubmed:25524396
  • Effect of coenzyme Q10 alone and its combination with metformin on streptozotocin-nicotinamide-induced diabetic nephropathy in ratsPubmed:25538335
  • Ameliorative potential of omega 3 fatty acids and HMG-CoA reductase inhibitors on experimentally-induced non-alcoholic steatohepatitisPubmed:25541279
  • A novel association between TGFb1 and ADAMTS4 in coronary artery disease: A new potential mechanism in the progression of atherosclerosis and diabetes.Pubmed:25592103
  • Targeting Apoptosis, Advanced Glycation End Products and Transforming Growth Factor-β1 by Biguanides and Resveratrol to Ameliorate Experimentally-induced Diabetic NephropathyPdf: Drugs
  • NIM811 downregulates transforming growth factorPubMed: 26573209
  • Salvia Miltiorrhiza Bge.f.alba Ameliorates the Progression of Monocrotaline-Induced Pulmonary Hypertension by Protecting Endothelial Injury in RatsPubMed: 26074502
  • Therapeutic effects of bone marrow-derived mesenchymal stem cells on radiation-induced lung injuryPubMed: 26717975
  • Epigallocatechin-3-gallate reduces tubular cell apoptosis in mice with ureteral obstructionPubMed: 25913488
  • Estrogen-deficient osteoporosis enhances the recruitment and activity of osteoclasts by breast cancer cellsPubMed: 26254457
  • Silymarin attenuates paraquat-induced lung injury via Nrf2-mediated pathway in vivo and in vitroPubMed: 26173462
  • Mesenchymal stromal cell-dependent reprogramming of Kupffer cells is mediated by TNF-α and PGE6 and is crucial for liver transplant tolerancePubMed: 25982596
  • MSX3 Switches Microglia Polarization and Protects from Inflammation-Induced DemyelinationPubMed: 25904788
  • The immunomodulating effect of seminal plasma on T cellsPubMed: 25799173
  • Camel Milk: Potential Utility as an Adjunctive Therapy to Peg-IFN/RBV in HCV-4 Infected Patients in EgyptPubMed: 26492130
  • Uighur medicine abnormal savda munzip (ASMq) suppresses expression of collagen and TGF-β1 with concomitant induce Smad7 in human hypertrophic scar …PubMed: 26309506
  • Development of a KLD-12 polypeptide/TGF-β1-tissue scaffold promoting the differentiation of mesenchymal stem cell into nucleus pulposus-like cells for treatment of intervertebral disc degeneration.PubMed: 25972996
  • Oxymatrine attenuates CCl 4-induced hepatic fibrosis via modulation of TLR4-dependent inflammatory and TGF-β1 signaling pathwaysPubmed:27179304
  • ReCavia (Guinea pig )lation of Viral Infection-induced Airway Remodeling Cytokine Production by the TLR3-EGFR Signaling Pathway in Human Bronchial Epithelial CellsPubmed:27075970
  • Human umbilical cord blood-mesenchymal stem cells transplantation renovates the ovarian surface epithelium in a rat model of premature ovarian failure: Possible direct and indirect effectsPubmed:27233913
  • Ameliorative Potential of Tamoxifen/Thymoquinone Combination in Patients with Breast Cancer: A Biochemical and Immunohistochemical Studyopen-access:1000102
  • Th17 Cell Related Cytokine Profiles in Narcolepsy and Other Types of Excessive Daytime Sleepinessarticle:15
  • Ameliorative potential of fluoxetine/raloxifene combination on experimentally induced breast cancerPubmed:26881735
  • TGF-β and Physiological Root Resorption of Deciduous Teeth.pubmed:28035998
  • Lymphocytic, cytokine and transcriptomic profiles in peripheral blood of dogs with atopic dermatitispubmed:27553600
  • Recombinant Mip-PilE-FlaA dominant epitopes vaccine candidate against Legionella pneumophila.pubmed:28366526
  • Gemcitabine treatment enhanced the anti-tumor effect of cytokine induced killer cells by depletion of CD4+CD25bri regulatory T cells.pubmed:27867030
  • Effect of Cardiac Resynchronization Therapy on Myocardial Fibrosis and Relevant Cytokines in a Canine Model With Experimental Heart Failure.pubmed:28127817
  • The protective role of vitamin D3 in a murine model of asthma via the suppression of TGF-β/Smad signaling and activation of the Nrf2/HO-1 pathway.pubmed:27484042
  • Amelioration of bleomycin-induced lung fibrosis in rats by valproic acid and butyrate: Role of nuclear factor kappa-B, proinflammatory cytokines and oxidative stresspubmed:27526269
  • Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced Cardiomyopathypubmed:27889753
  • The effect of midkine on growth factors and oxidative status in an experimental wound model in diabetic and healthy ratspubmed:28177680
  • Differential frequency of CD8+ T cell subsets in multiple sclerosis patients with various clinical patternspubmed:27467597
  • Elevated blood plasma levels of epinephrine, norepinephrine, tyrosine hydroxylase, TGFβ1, and TNFα associated with high-altitude pulmonary edema in an Indian population.pubmed:27540296
  • Effect of Indole-3-carbinol and/or Metformin on Female Patients with Ulcerative Colitis (Premalignant Condition): Role of Oxidative Stress, Apoptosis and Proinflammatory Cytokinesjcrt:5
  • Follistatin like-1 aggravates silica-induced mouse lung injuryPMC5428474
  • Effect of concomitant administration of coenzyme Q10 with sitagliptin on experimentally induced diabetic nephropathy in rats.pubmed:27841100
  • Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4+ T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling.pubmed:28032440
  • Imbalance of intestinal immune function in piglets infected by porcine circovirus type 2 during the fetal period.pubmed:28244338
  • Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in micepubmed:28250847
  • Ameliorative potential of linagliptin and/or calcipotriol on bleomycin-induced lung fibrosis: In vivo and in vitro studypubmed:28192751
  • Platelet-rich concentrate in serum free medium enhances osteogenic differentiation of bone marrow-derived human mesenchymal stromal cellspubmed:27651984
  • Crystalline Silica Promotes Rat Fibrocyte Differentiation in Vitro, and Fibrocytes Participate in Silicosis in Vivopubmed:29081339
  • Sildenafil protects against bile duct ligation induced hepaticfibrosis in rats: Potential role for silent information regulator 1 (SIRT1)pubmed:28974454
  • Glucosamine oral administration as an adjunct to hyaluronic acid injection in treating temporomandibular joint osteoarthritispubmed:28862770
  • Cytokine production in vitro and in rat model of colitis in response to Lactobacillus plantarum LS/07pubmed:28830068
  • The effect of feed supplementation with effective microorganisms(EM) on pro- and anti-inflammatory cytokine concentrations in pigspubmed:28549299
  • Extracellular Matrix Powder from Cultured Cartilage-Like Tissue as Cell Carriers for Cartilage RepairDOI:10.1039/C7TB00640C
  • Role of Gut-Derived Endotoxin on Type I Collagen Production in the Rat Pancreas after Chronic Alcohol Exposure pubmed:29121396
  • Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4 + T Helper 17(Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signallingdoi:10.1111
  • Plasma proteins as potential targets of abnormal Savda syndrome in asthma patients treated with unique Uighur prescriptionpubmed:28672924
  • Tumor-associated macrophages promote tumor metastasis via the TGF-β/SOX9 axis in non-small cell lung cancerpubmed:29245941
  • Studies on quality attributes of skimmed colostrum powderP-ISSN: 2349–8528
  • Follistatin like-1 aggravates silicainduced mouse lung injurypubmed:28341862
  • Cardioprotection against Heart Failure by Shenfu Injection via TGF-β/Smads Signaling Pathwaypubmed:28698735
  • Pigment epithelium‑derived factor protects human glomerular mesangial cells from diabetes via NOXO1‑iNOS suppression10.3892/mmr.2017.7563
  • Dexamethasone reduces serum level of IL-17 in Bleomycin-A5-induced rats model of pulmonary fibrosispubmed:28608724
  • Exendin-4 Induces Bone Marrow Stromal Cells Migration Through Bone Marrow-Derived Macrophages Polarization via PKA-STAT3 Signaling Pathwaypubmed:29216639
  • The promising effect of linagliptin and/or indole-3-carbinol on experimentally-induced polycystic ovarian syndrome.pubmed:28619389
  • Protective effect of aplysin on liver tissue and the gut microbiota in alcohol-fed ratspubmed:28622357
  • Qiliqiangxin Enhances Cardiac Glucose Metabolism and Improves Diastolic Function in Spontaneously Hypertensive Ratspubmed:28706558
  • TGF-β1 enhances phagocytic removal of neuron debris and neuronal survival by olfactory ensheathing cells via integrin/MFG-E8 signaling pathway10.1016/j.mcn.2017.08.006
  • Systemic and Tumor Th1 and Th2 Inflammatory Profile and Macrophages in Lung Cancer: Influence of Underlying Chronic Respiratory Disease.pubmed:27793775
  • Nose-to-brain delivery of insulin enhanced by a nanogel carrierPubmed:29196041
  • Lipopolysaccharide enhances TGF‐β1 signalling pathway and rat pancreatic fibrosisPubmed:29424488
  • Quercetin ameliorates pulmonary fibrosis by inhibiting SphK1/S1P signalingPubmed:29940125
  • Linagliptin potentiates the effect of l-dopa on the behavioural, biochemical and immunohistochemical changes in experimentally-induced Parkinsonism: Role of toll …Pubmed:29410018
  • A Novel scFv Anti-Aβ Antibody Reduces Pathological Impairments in APP/PS1 Transgenic Mice via Modulation of Inflammatory Cytokines and Aβ-related EnzymesPubmed:30062438
  • Nucleoside reverse transcriptase inhibitor-induced rat oocyte dysfunction and low fertility mediated by autophagyPubmed:29423092
  • Hypoxia-inducible factor-1α activates transforming growth factor-β1/Smad signaling and increases collagen deposition in dermal fibroblastsPubmed:29423039
  • Activated hepatic stellate cells promote epithelial-to-mesenchymal transition in hepatocellular carcinoma through transglutaminase 2-induced pseudohypoxia
  • Expression of Hif-1α, Nf-κb, and Vegf Genes in the Liver and Blood Serum Levels of HIF-1α, Erythropoietin, VEGF, TGF-β, 8-Isoprostane, and Corticosterone in Wistar Rats with High and Low Resistance to Hypoxia. Corticosterone in …Pubmed: 30353332
  • Mechanistic insights to the cardioprotective effect of Blueberry Nutraceutical Extract in Isoprenaline-Induced Cardiac Hypertrophy
  • Evaluation of T regulatory lymphocytes and serum concentration of selected cytokines in dogs with perianal tumors
  • Rhynchophylline attenuates allergic bronchial asthma by inhibiting transforming growth factor‑β1‑mediated Smad and mitogen‑activated protein kinase signaling …
  • Recombinant adiponectin alleviates abortion in mice by regulating Th17/Treg imbalance via p38MAPK-STAT5 pathwayPubmed: 30496353
  • ANP/NPRA Inhibits Epithelial-Mesenchymal Transition of Airway by Targeting Smad3 in Asthma
  • Anti-inflammatory and cognitive effects of interferon-β1a (IFNβ1a) in a rat model of Alzheimer's diseasePubmed: 30777084
  • Periostin contributes to renal and cardiac dysfunction in rats with chronic kidney disease: reduction of PPARαPubmed: 30890453
  • Astaxanthin ameliorates renal interstitial fibrosis and peritubular capillary rarefaction in unilateral ureteral obstructionPubmed: 30816496
  • Improving the anti-keloid outcomes through liposomes loading paclitaxel–cholesterol complexesPubmed: 30863067
  • THE ROLE OF ANTI-INFLAMMATORY CYTOKINES IN MEMORY PROCESSING IN A HEALTHY BRAIN
  • A novel xeno-free culture system for human retinal pigment epithelium cellsPubmed: 31024807
  • Low Degree Hyaluronic Acid Crosslinking Inducing the Release of TGF-Β1 in Conditioned Medium of Wharton's Jelly-Derived Stem Cells
  • TRAF-6 调控TGF-β1-Smad2/Smad3 信号通路在Graves 病免疫发病机制中的作用
  • Network pharmacology oriented study reveals inflammatory state-dependent dietary supplement hepatotoxicity responses in normal and diseased ratsPubmed: 31140472
  • CD19+ CD24hiCD38hi Regulatory B cells Involved in Hepatic Alveolar Hydatid Infection in HumansPubmed: 31308033
  • Pioglitazone attenuates kidney fibrosis via miR-21-5p modulationPubmed: 31254585
  • Hyperoside protects against heart failure-induced liver fibrosis in ratsPubmed: 31353051
  • Circulating Connective Tissue Growth Factor Is Associated with Diastolic Dysfunction in Patients with Diastolic Heart FailurePubmed: 31466059
  • Anti-inflammatory and antioxidant effects of muscarinic acetylcholine receptor (mAChR) activation in the rat hippocampusPubmed: 31578381
  • miR-181b-5p inhibits endothelial-mesenchymal transition in monocrotaline-induced pulmonary arterial hypertension by targeting endocan and TGFBR1Pubmed: 31734320
  • CD4+ CD25+ Tregs as dependent factor in the course of bleomycin-induced pulmonary fibrosis in micePubmed: 31678213
  • Lipoxin A4 inhibited the activation of Hepatic Stellate Cells-T6 cells by modulating profibrotic cytokines and NF-κB signaling pathwayPubmed: 31698141
  • Effects of thalidomide on Th17, Treg cells and TGF‐β1/Smad3 pathway in a mouse model of systemic sclerosisPubmed: 31840939
  • Altered expression of nuclear factor of activated T cells, Forkhead Box P3 and immune suppressive genes in regulatory T cells of generalized vitiligo patientsPubmed: 31917889
  • Rapamycin promotes endothelial–mesenchymal transition during stress-induced premature senescence through the activation of autophagyPubmed: 32164764
  • Soufeng Yuchuan decoction mitigates the ovalbumin-induced lung damage in a rat model of asthmaPubmed: 32036215
  • Isorhynchophylline exerts anti-asthma effects in mice by inhibiting the proliferation of airway smooth muscle cells: The involvement of miR-200a-mediated FOXC1/NF …Pubmed: 31733831
  • Vitamin D Attenuates Loss of Endothelial Biomarker Expression in Cardio-Endothelial CellsPubmed: 32235811
  • Levels of TGF-β1 in peri-miniscrew implant crevicular fluidPubmed: 32211284
  • Paeoniflorin accelerates foot wound healing in diabetic rats though activating the Nrf2 pathwayPubmed: 33166863
  • The anti-asthmatic potential of flavonol kaempferol in an experimental model of allergic airway inflammationPubmed: 33122672
  • Examination of androgenetic alopecia with serum biomarkersPubmed: 32969583
  • Soluble recombinant human thrombomodulin suppresses inflammation-induced gastrointestinal tumor growth in a murine peritonitis modelPubmed: 32767229
  • Hepatoprotective effect of Linagliptin against liver fibrosis induced by carbon tetrachloride in micePubmed: 32726558
  • Decreased suppression of CD8+ and CD4+ T cells by peripheral Regulatory T cells in Generalized Vitiligo due to reduced NFATC1 and FOXP3 proteinsPubmed: 32682346
  • Erlotinib can halt adenine induced nephrotoxicity in mice through modulating ERK1/2, STAT3, p53 and apoptotic pathwaysPubmed: 32661331
  • Chlorella vulgaris α-L-arabino-α-L-rhamno-α, β-D-galactan structure and mechanisms of its anti-inflammatory and anti-remodelling effectsPubmed: 32565301
  • Exosomal miR-532–5p from bone marrow mesenchymal stem cells reduce intervertebral disc degeneration by targeting RASSF5Pubmed: 32464126
  • The Sufficient Immunoregulatory Effect of Autologous Bone Marrow-Derived Mesenchymal Stem Cell Transplantation on Regulatory T Cells in Patients with …Pubmed: 32411794
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  • Nicotinamide riboside alleviates alcohol-induced depression-like behaviours in C57BL/6J mice by altering the intestinal microbiota associated with microglial …
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  • Menthacarin attenuates experimental colitis
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