Active Tumor Necrosis Factor Beta (TNFb)
LT; LTA; TNF-B; TNFSF1; Tumor Necrosis Factor Ligand Superfamily Member 1; Lymphotoxin Alpha
- UOM
- FOB US$ 330.00 US$ 824.00 US$ 1,648.00 US$ 4,944.00 US$ 12,360.00
- Quantity
Overview
Properties
- Product No.APA134Ra02
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsCell culture; Activity Assays.
Research use only - DownloadInstruction Manual
- CategoryCytokineTumor immunityInfection immunity
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder, Purity > 90%
- Isoelectric Point10.2
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Activity test

TNF-β, a member of the tumor necrosis factor family, is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells . The biological effects of TNF-β are very similar to TNF-α,due to the similarity of molecular structure and the receptors.As reported, TNF-α could inhibit the proliferation and induce apoptosis of A549 cells,therefore, A549 cells were seeded into triplicate wells of 96-well plates at a density of 4,000 cells/well with 5% serum standard DMEM including various concentrations of recombinant rat TNF-β. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 2 hours at 37℃. Proliferation of A549 cells after incubation with TNF-β for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant mouse TNF-β for 48h. The result was shown in Figure 2. It was obvious that TNF-β significantly inhibit cell viability of A549 cells. The ED50 is 2.5μg/ml.

Figure 2. Inhibition of A549 cells proliferation after stimulated with TNF-β
Usage
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Buffer
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Spike RBD Protein (S-RBD)
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Protein G
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Protein A
Citations
- Simvastatin attenuates sympathetic hyperinnervation to prevent atrial fibrillation during the postmyocardial infarction remodeling processPubMed: 22984252
- Effectiveness of pure argon for renal transplant preservation in a preclinical pig model of heterotopic autotransplantationPubmed:26847569
- Protective Role of Bone Marrow Derived Mesenchymal Stem Cells-Conditioned Medium in the Infarcted Myocardium: The Potential Role of Selected CytokinesIJST-2328-3548-04-803
- Effect of Multi-Microbial Probiotic Formulation Bokashi on Pro-and Anti-Inflammatory Cytokines Profile in the Serum, Colostrum and Milk of Sows, and in a …Pubmed:29305686
- Fasciola gigantica excretory-secretory products (FgESPs) modulate the differentiation and immune functions of buffalo dendritic cells through a mechanism …Pubmed: 32680553
- Microbiome changes in patients with chronic heart failure with preserved ejection fraction correlate with fibrosis markers: Description of a Russian cohort
- Elevated lymphotoxin-¦Á (TNF¦Â) is associated with intervertebral disc degeneration33441130