CLIA Kit for Mucin 5 Subtype AC (MUC5AC) Mus musculus (Mouse) Sandwich CLIA

TBM; MUC5; LeB; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtypes A And C,Tracheobronchial/Gastric; Gastric mucin; Lewis B blood group antigen; Major airway glycoprotein

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  • CLIA Kit for Mucin 5 Subtype AC (MUC5AC) Packages (Simulation)
  • CLIA Kit for Mucin 5 Subtype AC (MUC5AC) Packages (Simulation)
  • CLIA Kit for Mucin 5 Subtype AC (MUC5AC) Results demonstration
  • SCA756Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Mucin 5 Subtype AC (MUC5AC) and the recovery rates were calculated by comparing the measured value to the expected amount of Mucin 5 Subtype AC (MUC5AC) in samples.

Matrix Recovery range (%) Average(%)
EDTA plasma(n=5) 79-104 92
heparin plasma(n=5) 92-101 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mucin 5 Subtype AC (MUC5AC) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mucin 5 Subtype AC (MUC5AC) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mucin 5 Subtype AC (MUC5AC) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
EDTA plasma(n=5) 78-102% 86-101% 92-105% 79-94%
heparin plasma(n=5) 81-95% 93-101% 86-94% 82-95%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

CLIA Kit for Mucin 5 Subtype AC (MUC5AC)

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Mucin 5 Subtype AC (MUC5AC). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Mucin 5 Subtype AC (MUC5AC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Mucin 5 Subtype AC (MUC5AC) level in the sample or standard.;

Citations

  • Pancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas.PubMed: 20717734
  • Entrapment of Conjunctival Goblet Cells by Desiccation-Induced CornificationPubMed: 21421863
  • Role of aquaporin 5 in antigen-induced airway inflammation and mucous hyperproduction in micePubMed: 20550619
  • Homeostatic control of conjunctival mucosal goblet cells by NKT-derived IL-13Nature: 201082a
  • Naringin attenuates EGF-induced MUC5AC secretion in A549 cells by suppressing the cooperative activities of MAPKs-AP-1 and IKKs-IκB-NF-κB signaling pathwaysScienceDirect: S0014299912005523
  • Baicalein Reduces Airway Injury in Allergen and IL-13 Induced Airway InflammationPubMed: PMC3639905
  • Pyocyanin-induced mucin production is associated with redox modification of FOXA2PubMed: PMC3765780
  • A novel serum marker for biliary tract cancer: Diagnostic and prognostic values of quantitative evaluation of serum mucin 5AC (MUC5AC)Pubmed: 24468034
  • The human cathelicidin LL-37 enhances airway mucus production in chronic obstructive pulmonary diseasePubmed:24291709
  • Proteomic analysis of cancer and mesothelial cells reveals an increase in Mucin 5AC during ovarian cancer and peritoneal interactionPubmed:24726482
  • The human Cathelicidin LL-37 induces MUC5AC mucin production by airway epithelial cells via TACE-TGF-α-EGFR pathwayPubmed:24901072
  • Assessment of bile and serum mucin5AC in cholangiocarcinoma: Diagnostic performance and biologic significancePubmed:25151557
  • Leukotriene E 4 induces MUC5AC release from human airway epithelial NCI-H292 cellsPubmed:25838093
  • Secreted mucins and airway bacterial colonization in non-CF bronchiectasisPubMed: 26172851
  • Isabela Casagrande JeremiasTeses: Disponiveis
  • Mucin Secretion In Asthmatics With Osmotic Airway Hyperresponsivenessdoi:10.1164
  • ReCavia (Guinea pig )lation of Viral Infection-induced Airway Remodeling Cytokine Production by the TLR3-EGFR Signaling Pathway in Human Bronchial Epithelial CellsPubmed:27075970
  • The influence of long-term corneal contact lens wearing on the stability and quality of tear film in Qinghaifiles:ijcem0020163.pdf
  • Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonizationdoi:10.1513
  • Impact of Cigarette Smoking on Tear Function and Correlation between Conjunctival Goblet Cells and Tear MUC5AC Concentration in Office WorkersPubmed:27297822
  • Interleukin-33 induces mucin gene expression and goblet cell hyperplasia in human nasalepithelial cells.pubmed:27776277
  • Mutating the CX3C Motif in the G Protein Should Make a Live Respiratory Syncytial Virus Vaccine Safer and More Effective.pubmed:28275196
  • Peroxiredoxin 6 suppresses Muc5ac overproduction in LPS-induced airway inflammation through H2O2-EGFR-MAPK signaling pathway.pubmed:27884794
  • Beneficial effects of prebiotic Saccharomyces cerevisiae mannan on allergic asthma mouse modelspubmed:28835901
  • Efficacy of Sodium Hyaluronate in Murine Diabetic Ocular Surface Diseasespubmed:28644234
  • Clarithromycin inhibits TNF-α-induced MUC5AC mucin gene expression via the MKP-1-p38MAPK-dependent pathwaypubmed:28550735
  • Секреторно-экссудативная реакция слизистой носа на воздействие холодного воздуха у больных бронхиальной астмой10.20538/1682-0363-2017-2-146-158
  • An adherent mucus layer attenuates the genotoxic effect of colibactin. pubmed:29156489
  • IL-4-induced caveolin-1-containing lipid rafts aggregation contributes to MUC5AC synthesis in bronchial epithelial cellspubmed:28931396
  • Oxytetracycline Inhibits Mucus Secretion and Inflammation in Human Airway Epithelial Cells.pubmed:28578323
  • Th2 cytokines orchestrate the secretion of MUC 5 AC and MUC 5B in IL‐5 positive chronic rhinosinusitis with nasal polypsPubmed:29802623
  • Salvianolic acid B improves airway hyperresponsiveness by inhibiting MUC5AC overproduction associated with Erk1/2/P38 signalingPubmed:29382534
  • Plantamajoside Inhibits Lipopolysaccharide-Induced MUC5AC Expression and Inflammation through Suppressing the PI3K/Akt and NF-κB Signaling Pathways in …Pubmed:29349683
  • In vivo and in vitro investigation of anti-inflammatory and mucus-regulatory activities of a fixed combination of thyme and primula extractsPubmed:29698781
  • Profiling of healthy and asthmatic airway smooth muscle cells following IL-1β treatment: a novel role for CCL20 in chronic mucus hyper-secretionPubmed:29946002
  • Development of a highly sensitive and reliable enzyme-linked immunosorbent assay for MUC5aC in human tears extracted from schirmer stripsPubmed: 30214141
  • Increased expression of long non-coding RNA SNHG16 correlates with tumor progression and poor prognosis in non-small cell lung cancerPubmed: 30287374
  • The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung diseasePubmed: 30573494
  • Regulation effects of naringin on diesel particulate matter-induced abnormal airway surface liquid secretionPubmed: 31301536
  • Regional comparison of Goblet cell number and Area in exposed and covered Dry eyes and their Correlation with Tear MUC5ACPubmed: 32076085
  • TLR3 inhibitor and tyrosine kinase inhibitor attenuate cigarette smoke/poly I: C-induced airway inflammation and remodeling by the EGFR/TLR3/MAPK …Pubmed: 33068589
  • Effective-component compatibility of Bufei Yishen formula II inhibits mucus hypersecretion of chronic obstructive pulmonary disease rats by regulating EGFR/PI3K …Pubmed: 32344236
  • Less Airway Inflammation and Goblet Cell Metaplasia in an IL-33-Induced Asthma Model of Leptin-Deficient Obese Mice
  • The regulatory role of SFRP5/WNT5A axis in allergic rhinitis through inhibiting JNK pathway activation and lowering mucin generation in human nasal?¡­33285209
  • Porphyromonas gingivalis gingipains potentially affect MUC5AC gene expression and protein levels in respiratory epithelial cells33332733
  • Generation of functional conjunctival epithelium, including goblet cells, from human iPSCs33535050

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