CLIA Kit for Toll Like Receptor 4 (TLR4) Mus musculus (Mouse) Sandwich CLIA

CD284; TOLL; HToll

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  • CLIA Kit for Toll Like Receptor 4 (TLR4) Packages (Simulation)
  • CLIA Kit for Toll Like Receptor 4 (TLR4) Packages (Simulation)
  • CLIA Kit for Toll Like Receptor 4 (TLR4) Results demonstration
  • SCA753Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Toll Like Receptor 4 (TLR4) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Toll Like Receptor 4 (TLR4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

CLIA Kit for Toll Like Receptor 4 (TLR4)

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Toll Like Receptor 4 (TLR4). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Toll Like Receptor 4 (TLR4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Toll Like Receptor 4 (TLR4) level in the sample or standard.;

Citations

  • Expression of soluble Toll-like receptors in pleural effusionsCMJ: 2010823483999506297
  • Amniotic fluid soluble Toll-like receptor 4 in pregnancies complicated by preterm prelabor rupture of the membranesInforma: 14767058.2011.626821
  • Baicalein Reduces Airway Injury in Allergen and IL-13 Induced Airway InflammationPubMed: PMC3639905
  • The association between precancerous gastric lesions and serum pepsinogens, serum gastrin, vascular endothelial growth factor, serum interleukin-1 Beta, serum toll-like receptor-4 levels and Helicobacter pylori Cag A statusPubmed: 23137754
  • Toll-Like Receptor 2 in Serum: a Potential Diagnostic Marker of Prosthetic Joint Infection?Pubmed: 24478497
  • CO2 pneumoperitoneum increases secretory IgA levels in the gut compared with laparotomy in an experimental animal model.Pubmed: 24442682
  • Soluble toll-like receptor 4 reversed attenuating effect of Chinese herbal Xiao-Qing-Long-Tang on allergen induced nerve growth factor and thymic stromal lymphopoietinPubmed: 24223644
  • Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitisPubmed:24992835
  • Cytokines and Toll-Like Receptors in the Immune Response to Influenza VaccinationPubmed:25248345
  • Beta-arrestin-2 negatively modulates inflammation response in mouse chondrocytes induced by 4-mer hyaluronan oligosaccharidePubmed:25318610
  • Effects of Korean Red Ginseng (Panax ginseng), urushiol (Rhus vernicifera Stokes), and probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus acidophilus R0052) on the gut–liver axis of alcoholic liver diseasePubmed:25378990
  • The discriminative capacity of soluble Toll-like receptor (sTLR)2 and sTLR4 in inflammatory diseasesPubmed:25406630
  • Procoagulatory State in Inflammatory Bowel Diseases Is Promoted by Impaired Intestinal Barrier FunctionPubmed:25767508
  • Efficacy of rifaximin on circulating endotoxins and cytokines in patients with nonalcoholic fatty liver diseasePubMed: 26043290
  • Cathelicidin related antimicrobial peptide, laminin, Toll-like receptors and chemokines levels in experimental hypersensitivity pneumonitis in micePubMed: 25834936
  • Effects of Hyriopsis cumingii Polysaccharides on Mice Immunologic Receptor, Transcription Factor, and CytokinePubmed:27061742
  • Soluble Toll-Like Receptors 2 and 4 in Cerebrospinal Fluid of Patients with Acute Hydrocephalus followingAneurysmal Subarachnoid Haemorrhage.Pubmed:27223696
  • The Effect of Rosuvastatin on Markers of Immune Activation in Treatment-Naive Human Immunodeficiency Virus-PatientsPubmed:26835476
  • Impact of Saccharomyces cerevisiae fermentation product and subacute ruminal acidosis on production, inflammation, and fermentation in the rumen and hindCavia (Guinea pig )t of dairy cowsscience:S0377840115300493
  • Garlic active constituent s-allyl cysteine protects against lipopolysaccharide-induced cognitive deficits in the rat: Possible involved mechanisms.pubmed:27915041
  • S-allyl cysteine ameliorates cognitive deficits in streptozotocin-diabetic rats via suppression of oxidative stress, inflammation, and acetylcholinesterase.pubmed:27887948
  • Ellagic acid ameliorates learning and memory deficits in a rat model of Alzheimer's disease: an exploration of underlying mechanisms.pubmed:28303372
  • НОСИТЕЛЬСТВО ПОЛИМОРФИЗМА Thr399Ile и Asp299Gly ГЕНА TLR4 И УРОВЕНЬ TLR4 В ОПУХОЛЕВОЙ ТКАНИ У БОЛЬНЫХ ПРОЛИФЕРАТИВНЫМИ ЗАБОЛЕВАНИЯМИ МОЛОЧНОЙ ЖЕЛЕЗЫ8.pdf
  • Acetyl-l-carnitine protects dopaminergic nigrostriatal pathway in 6-hydroxydopamine-induced model of Parkinson's disease in the rat.pubmed:28199883
  • Soluble TLR2 and 4 concentrations in cerebrospinal fluid in HIV/SIV-relatedneuropathological conditions.pubmed:27882497
  • Toll-like receptors and their soluble forms differ in the knee and thumb basal osteoarthritic jointspubmed:28093922
  • Ameliorative potential of linagliptin and/or calcipotriol on bleomycin-induced lung fibrosis: In vivo and in vitro studypubmed:28192751
  • Toll-Like Receptor 4 Inhibition Improves Oxidative Stress and Mitochondrial Health in Isoproterenol-Induced Cardiac Hypertrophy in Ratspubmed:28690610
  • Probiotic Lactobacillus acidophilus bacteria or synthetic TLR2 agonist boost the growth of chicken embryo intestinal organoids in cultures comprising epithelial cells and myofibroblastsDOI: 10.1016/j.cimid.2017.06.002
  • Probiotic Lactobacillus acidophilus bacteria or synthetic TLR2 agonist boost the growth of chicken embryo intestinal organoids in cultures comprising epithelial cells and myofibroblasts10.1016/j.cimid.2017.06.002
  • Protective effects of sinomenine against LPS-induced inflammation in piglets.pubmed:28757275
  • Soy isoflavone genistein attenuates lipopolysaccharide-induced cognitive impairments in the rat via exerting anti-oxidative and anti-inflammatory effectspubmed:29102164
  • Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanismspubmed:28061403
  • The soluble Form of Toll-like receptor 2 is elevated in serum of Multiple sclerosis Patients: a novel Potential Disease BiomarkerPubmed:29593720
  • Specific alterations in the circulating levels of the SIRT1, TLR4, and IL7 proteins in patients with dementiaPubmed:30071285
  • Protective Effects of Fibroblast Growth Factor 21 Against Amyloid-Beta-Induced Toxicity in SH-SY5Y CellsPubmed:29869772
  • Rutin, a quercetin glycoside, alleviates acute endotoxemic kidney injury in C57BL/6 mice via suppression of inflammation and up-regulation of antioxidants and SIRT1Pubmed:29920283
  • Crocin reverses unilateral renal ischemia reperfusion injury-induced augmentation of oxidative stress and toll like receptor-4 activityPubmed:29625388
  • Linagliptin potentiates the effect of l-dopa on the behavioural, biochemical and immunohistochemical changes in experimentally-induced Parkinsonism: Role of toll …Pubmed:29410018
  • Increased serum levels of high mobility group protein B1 and calprotectin in pre‐eclampsiaPubmed:29569400
  • Increased TLR4 and TREM-1 expression on monocytes and neutrophils in preterm birth: further evidence of a proinflammatory statePubmed:29534643
  • ТLR4-РІВНІ У ХВОРИХ НА ПОДАГРУ: ЗВ'ЯЗОК З АКТИВНІСТЮ ЗАХВОРЮВАННЯ:
  • Combined effect of anti‐high‐mobility group box‐1 monoclonal antibody and peramivir against influenza A virus‐induced pneumonia in micePubmed: 30281823
  • Berberine ameliorates lipopolysaccharide-induced learning and memory deficit in the rat: insights into underlying molecular mechanismsPubmed: 30456649
  • The HMGB1‑RAGE/TLR‑TNF‑α signaling pathway may contribute to kidney injury induced by hypoxia
  • Protective effect of sesamin in lipopolysaccharide-induced mouse model of acute kidney injury via attenuation of oxidative stress, inflammation, and apoptosisPubmed: 30488751
  • S-allyl cysteine protects against lipopolysaccharide-induced acute kidney injury in the C57BL/6 mouse strain: Involvement of oxidative stress and inflammationPubmed: 30665040
  • Opuntioside, opuntiol and its metallic nanoparticles attenuate adjuvant-induced arthritis: Novel suppressors of Toll-like receptors-2 and-4
  • Circulating microbiota-derived metabolites: a “liquid biopsy?Pubmed: 31388096
  • Deregulation of TLR4 signaling pathway characterizes Bicuspid Aortic valve syndromePubmed: 31363123
  • Crocin attenuates lung inflammation and pulmonary vascular dysfunction in a rat model of bleomycin-induced pulmonary fibrosisPubmed: 31465731
  • Targeting oxidative stress, proinflammatory cytokines, apoptosis and toll like receptor 4 by empagliflozin to ameliorate bleomycin-induced lung fibrosisPubmed: 31600583
  • Particulate Shiga Toxin 2 in Blood is Associated to the Development of Hemolytic Uremic Syndrome in ChildrenPubmed: 31858520
  • Effects of fermented ginseng on the gut microbiota and immunity of rats with antibiotic-associated diarrheaPubmed: 33217518
  • Relationship between IL-8 Circulating Levels and TLR2 Hepatic Expression in Women with Morbid Obesity and Nonalcoholic SteatohepatitisPubmed: 32545403
  • Diosgenin Attenuates Cognitive Impairment in Streptozotocin-Induced Diabetic Rats: Underlying MechanismsPubmed: 32526752
  • S‐allyl cysteine, an active ingredient of garlic, attenuates acute liver dysfunction induced by lipopolysaccharide/ d‐galactosamine in mouse: Underlying mechanismsPubmed: 32453893
  • Protective effect of diosgenin on LPS/D-Gal-induced acute liver failure in C57BL/6 micePubmed: 32389705
  • FEATURES OF MYOCARDIAL REMODELING IN MEN WITH POST-INFARCTION CARDIOSCLEROSIS AND ITS RELATIONSHIP WITH TOLL-LIKE …
  • Putative anti-inflammatory, antioxidant, and anti-apoptotic roles of the natural tissue guardian methyl palmitate against isoproterenol-induced myocardial …
  • The role of Toll-like receptors-4 in the pathogenesis of development of anemia of inflammation in young children
  • Evaluation of heat shock protein 70 and toll-like receptor 4 expression in gingival crevicular fluid in response to orthodontic forces34091792
  • Tissue transglutaminase is involved in the inflammatory processes of active chronic gastritis17539352
  • Esculetin Alleviates Acute Liver Failure following Lipopolysaccharide/D-Galactosamine in Male C57BL/6 Mice
  • A value of soluble Toll‐like receptor 2 and 4 in vaginally obtained amniotic fluid for the prediction of histological chorioamnionitis34244993
  • Expression of Jejunal Taste Receptors in Women with Morbid Obesity34371946
  • Nifuroxazide-loaded cubosomes exhibit an advancement in pulmonary delivery and attenuate bleomycin-induced lung fibrosis by regulating the STAT3 and …Pubmed:35220029
  • Coomassie brilliant blue G-250 dye attenuates bleomycin-induced lung fibrosis by regulating the NF-κB and NLRP3 crosstalk: A novel approach for filling an …Pubmed:35202914
  • Carbocisteine as a Modulator of Nrf2/HO-1 and NFκB Interplay in Rats: New Inspiration for the Revival of an Old Drug for Treating Ulcerative Colitis. Front …Pubmed:35754464
  • Lipocalin, Resistin and Gut Microbiota-Derived Propionate Could Be Used to Predict Metabolic Bariatric Surgery Selected Outcomes
  • Vascular Calcification and the Gut and Blood Microbiome in Chronic Kidney Disease Patients on Peritoneal Dialysis: A Pilot Study

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