Anti-Complement Component 4 (C4) Polyclonal Antibody Mus musculus (Mouse) Protein, Antibody, Assaykit, Service

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Overview
Properties
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Excitation LaserNone
  • Vol. per test1-5 µl
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid
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  • Anti-Complement Component 4 (C4) Polyclonal Antibody Packages (Simulation)
  • Anti-Complement Component 4 (C4) Polyclonal Antibody Packages (Simulation)
  • Anti-Complement Component 4 (C4) Polyclonal Antibody HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-mouse C4 Polyclonal Antibody (Catalog # FAA888Mu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Usage

For flow cytometry, the suggested use of this reagent is 1-5 µl per 10^6 cells in 100 µl volume.
Optimal working dilutions could be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Giveaways

Citations

  • Complement activation contributes to the injury and outcome of kidney in human anti-glomerular basement membrane disease.PubMed: 22941511
  • The suppression of Brd4 inhibits peripheral plasma cell differentiation and exhibits therapeutic potential for systemic lupus erythematosus

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