Anti-Histone H3 (H3) Monoclonal Antibody 
Overview
Properties
- Product No.CAA285Hu22
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsLoading Control of WB.
Research use only - Downloadn/a
- CategoryDevelopmental science
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid, Concentration 1mg/mL
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Packages (Simulation)
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Packages (Simulation)
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Western Blot; Sample: A549 cell lysate
Primary Ab: 0.2µg/ml Mouse Anti-Human H3 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Samples: Lane1: 293F cell lysate; Lane2: Jurkat cell lysate;
Primary Ab: 0.5µg/ml Mouse Anti-Human H3 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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ISO9001: 2008, ISO13485: 2003 Registered
Specifity
The antibody is a mouse monoclonal antibody raised against H3. It has been selected for its ability to recognize H3 in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Organism Species More: Oryctolagus cuniculus (Rabbit), Canis familiaris; Canine (Dog)Giveaways
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Citations
- A Novel Anti-Histone H1 Monoclonal Antibody, SSV Monoclonal Antibody, Improves Lung Injury and Survival in a Mouse Model of Lipopolysaccharide-Induced Sepsis-Like SyndromePubMed: 25649890
- Proteomic analysis reveals USP7 as a novel regulator of palmitic acid-induced hepatocellular carcinoma cell deathPubmed:35732625