Anti-Histone H3 (H3) Polyclonal Antibody Homo sapiens (Human) Control antibody

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Overview
Properties
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Anti-Histone H3 (H3) Polyclonal Antibody Packages (Simulation)
  • Anti-Histone H3 (H3) Polyclonal Antibody Packages (Simulation)
  • Anti-Histone H3 (H3) Polyclonal Antibody DAB staining on IHC-P; Samples: Human Small intestine Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human H3 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Anti-Histone H3 (H3) Polyclonal Antibody DAB staining on IHC-P;
    Samples: Human Cerebrum Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human H3 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Anti-Histone H3 (H3) Polyclonal Antibody DAB staining on IHC-P;
    Samples: Human Pancreas Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human H3 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Anti-Histone H3 (H3) Polyclonal Antibody DAB staining on IHC-P;
    Samples: Human Spleen Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human H3 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Anti-Histone H3 (H3) Polyclonal Antibody DAB staining on IHC-P;
    Samples: Human Placenta Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human H3 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Anti-Histone H3 (H3) Polyclonal Antibody DAB staining on IHC-P;
    Samples: Human Colon Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human H3 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Anti-Histone H3 (H3) Polyclonal Antibody DAB staining on IHC-P;
    Sample: Human Cerebrum Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Human H3 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Anti-Histone H3 (H3) Polyclonal Antibody DAB staining on IHC-P;
    Sample: Human Small intestine Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Human H3 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Anti-Histone H3 (H3) Polyclonal Antibody DAB staining on IHC-P;
    Sample: Human Spleen Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Human H3 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • CAA285Hu01.jpg Figure. Western Blot; Sample: Recombinant H3, Human.
  • Anti-Histone H3 (H3) Polyclonal Antibody Western Blot; Sample: Lane1: Human Lung lysate; Lane2: Human Placenta lysate; Lane3: A549 cell lysate; Lane4: HepG2 cell lysate
    Primary Ab: 0.005µg/ml Rabbit Anti-Human H3 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against H3. It has been selected for its ability to recognize H3 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2ug/ml
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Rhesus monkey (Simian), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Equus caballus; Equine (Horse), Chicken (Gallus)

Giveaways

Citations

  • A Novel Anti-Histone H1 Monoclonal Antibody, SSV Monoclonal Antibody, Improves Lung Injury and Survival in a Mouse Model of Lipopolysaccharide-Induced Sepsis-Like SyndromePubMed: 25649890
  • Proteomic analysis reveals USP7 as a novel regulator of palmitic acid-induced hepatocellular carcinoma cell deathPubmed:35732625

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