Polyclonal Antibody to Complement Component 4a (C4a) Mus musculus (Mouse) Polyclonal antibody

C4a anaphylatoxin

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA389Mu02-Recombinant Complement Component 4a (C4a)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Complement Component 4a (C4a) Packages (Simulation)
  • Polyclonal Antibody to Complement Component 4a (C4a) Packages (Simulation)
  • Polyclonal Antibody to Complement Component 4a (C4a) DAB staining on IHC-P; Samples: Mouse Liver Tissue; Primary Ab: 30µg/ml Rabbit Anti-Mouse C4a Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Complement Component 4a (C4a) DAB staining on IHC-P;
    Sample: Mouse Kidney Tissue;
    Primary Ab: 30µg/ml Rabbit Anti-Mouse C4a Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
    Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Complement Component 4a (C4a) DAB staining on IHC-P;
    Sample: Mouse Colon Tissue;
    Primary Ab: 30µg/ml Rabbit Anti-Mouse C4a Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
    Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Complement Component 4a (C4a) DAB staining on IHC-P;
    Samples: Mouse Small intestine Tissue;
    Primary Ab: 30µg/ml Rabbit Anti-Mouse C4a Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Complement Component 4a (C4a) DAB staining on IHC-P;
    Sample: Mouse Liver Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Mouse C4a Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA389Mu01.jpg Western Blot; Sample: Lane1: Mouse Plasma; Lane2: Rat Serum; Lane3: Rat Plasma Primary Ab: 1μg/ml Rabbit Anti-Mouse C4a Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) Selected
  • Polyclonal Antibody to Complement Component 4a (C4a) Western Blot; Sample: Mouse Placenta lysate
    Primary Ab: 0.2µg/ml Rabbit Anti-Mouse C4a Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19) Selected
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against C4a. It has been selected for its ability to recognize C4a in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Rattus norvegicus (Rat)

Giveaways

Citations

  • Circulation levels of acute phase proteins in patients with Takayasu arteritisPubMed: 20100644
  • Identification of Altered Plasma Proteins by Proteomic Study in Valvular Heart Diseases and the Potential Clinical SignificancePubMed: PMC3754973
  • Higher Serum Angiotensinogen Is an Indicator of IgA Vasculitis with Nephritis Revealed by Comparative Proteomes AnalysisPubMed: 26098644
  • Plasma Protein Biomarkers of Hepatocellular Carcinoma in HCV-Infected Alcoholic Patients with CirrhosisPubMed: 25789864
  • Quantitative analysis of hydroxyapatite-binding plasma proteins in genotyped individuals with late-stage age-related macular degenerationPubmed:29580721
  • Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2JPubmed: 32585848
  • Maternal Serum Proteomic Profiles of Pregnant Women With Type 1 Diabetes34940989

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