Polyclonal Antibody to Lipopolysaccharide (LPS) Pan-species (General) Polyclonal antibody

LOS; Lipoglycans; Lipooligosaccharide; Lipo-Oligosaccharide; Endotoxin

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen CPB526Ge21-OVA Conjugated Lipopolysaccharide (LPS)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Lipopolysaccharide (LPS) Packages (Simulation)
  • Polyclonal Antibody to Lipopolysaccharide (LPS) Packages (Simulation)
  • Polyclonal Antibody to Lipopolysaccharide (LPS) DAB staining on IHC-P; Sample: Rat Colon Tissue Primary Ab: 10µg/ml Rabbit Anti-Multi-species LPS Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Lipopolysaccharide (LPS) DAB staining on IHC-P;
    Sample: Rat Small intestine Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Multi-species LPS Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against LPS. It has been selected for its ability to recognize LPS in ELISA and CLIA.

Usage

Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Pan-species (General)

Giveaways

Citations

  • Particulate matter air pollution disrupts endothelial cell barrier via calpain-mediated tight junction protein degradationPubMed: 22931549
  • Hemoglobin induces inflammation after preterm intraventricular hemorrhage by methemoglobin formationPubMed: PMC3750409
  • Antidiabetic effect of Lactobacillus casei CCFM0412 in high-fat-fed, streptozotocin-induced type 2 diabetic mice ScienceDirect: S0899900714001567
  • Effects of Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036 on Hepatic Steatosis in Zucker RatsPubmed:24852284
  • Immunological Activity Difference between Native Calreticulin Monomers and OligomersPubmed:25171171
  • Antidiabetic effect of Lactobacillus casei CCFM0412 on mice with type 2 diabetes induced by a high-fat diet and streptozotocinPubmed:25102821
  • Oral administration of Lactobacillus rhamnosus CCFM0528 improves glucose tolerance and cytokine secretion in high-fat-fed, streptozotocin-induced type 2 diabetic …Sciencedirect:S1756464614002138
  • Is there interaction between gut microbial profile and cardiovascular risk in chronic kidney disease patients?PubMed: 25865191
  • Effects of probiotics (cultured Lactobacillus subtilis/Streptococcus faecium) in the treatment of alcoholic hepatitis: randomized-controlled multicenter studyPubMed: 26302024
  • The effects of female sexual steroids on gastric function and barrier resistance of gastrointestinal tract following traumatic brain injuryPubMed: 25709342
  • Fecal menaquinone profiles of overweight adults are associated with gut microbiota composition during a gut microbiota–targeted dietary interventionPubMed26016865 :
  • Deficiency of intestinal mucin-2 protects mice from diet-induced fatty liver disease and obesityPubMed: 26702135
  • Oxymatrine attenuates CCl 4-induced hepatic fibrosis via modulation of TLR4-dependent inflammatory and TGF-β1 signaling pathwaysPubmed:27179304
  • Portal vein thrombosis in cirrhosis is not associated with intestinal barrier disruption or increased platelet aggregabilityPubmed:27160816
  • Administration of probiotic mixture DM# 1 ameliorated 5-fluorouracil-induced intestinal mucositis and dysbiosis in ratsscience:S0899900716300740
  • Inhibition of cyclooxygenase-2 alleviates liver cirrhosis via improvement of the dysfunctional Cavia (Guinea pig )t-liver axis in ratsPubmed:27056726
  • Food combination based on a pre-hispanic Mexican diet decreases metabolic and cognitive abnormalities and gut microbiota dysbiosis caused by a sucrose-enriched high-fat diet in rats.pubmed:27352915
  • Administration of probiotic mixture DM# 1 ameliorated 5-fluorouracil–induced intestinal mucositis and dysbiosis in ratsarticle:S0899-9007(16)30074-0
  • Modulation of gut microbiota contributes to curcumin-mediated attenuation of hepatic steatosis in ratspubmed:28341485
  • Relationship between plasma levels of zonulin, bacterial lipopolysaccharides, d‑lactate and markers of inflammation in haemodialysis patientspubmed:28044237
  • Administration of probiotic mixture DM#1 ameliorated 5-fluorouracil-induced intestinal mucositis and dysbiosis in ratspubmed:27427511
  • Co-supplementation of isomalto-oligosaccharides potentiates metabolic health benefits of polyphenol-rich cranberry extract in high fat diet-fed mice via enhanced gut butyrate productiondoi: 10.1007/s00394-017-1561-5
  • Coadministration of isomalto-oligosaccharides augments metabolic health benefits of cinnamaldehyde in high fat diet fed mice.pubmed:28799667
  • Nopal (Opuntia ficus indica) protects from metabolic endotoxemia by modifying gut microbiota in obese rats fed high fat/sucrose diet.pubmed:28680065
  • Gastric acid suppression promotes alcoholic liver disease by inducing overgrowth of intestinal Enterococcus.pubmed:29038503
  • Food combination based on a pre-hispanic Mexican diet decreases metabolic and cognitive abnormalities and gut microbiota dysbiosis caused by a sucrose-enriched high-fat diet in rats10.1002/mnfr.201501023
  • Differential Effect of Sucrose and Fructose in Combination with a High Fat Diet on Intestinal Microbiota and Kidney Oxidative Stresspubmed:28420148
  • House Dust Mites Induce Production of Endothelin-1 and Matrix Metalloproteinase-9 in Keratinocytes via Proteinase-Activated Receptor-2 Activationpubmed:28586781
  • Long‐Term Genistein Consumption Modifies Gut Microbiota, Improving Glucose Metabolism, Metabolic Endotoxaemia and Cognitive Function in Mice Fed a High‐Fat …Pubmed:29979819
  • IgG Immunocomplexes Sensitize Human Monocytes for Inflammatory Hyperactivity via Transcriptomic and Epigenetic Reprogramming in Rheumatoid ArthritisPubmed:29712771
  • AWRK6, A Synthetic Cationic Peptide Derived from Antimicrobial Peptide Dybowskin-2CDYa, Inhibits Lipopolysaccharide-Induced Inflammatory ResponsePubmed:29463000
  • Effects of probiotic supplementation on inflammatory biomarkers and uremic toxins in non-dialysis chronic kidney patients: A double-blind, randomized, placebo …10.1016:j.jff.2018.05.018
  • Effect of systemic administration of lipopolysaccharides derived from on gene expression in mice kidneyPubmed:29388058
  • The Role of Intestinal C‐type Regenerating Islet Derived‐3 Lectins for Nonalcoholic SteatohepatitisPubmed:29619418
  • アトピー性皮膚炎の痒みの発症機序に関する研究
  • Repression of TXNIP–NLRP3 axis restores intestinal barrier function via inhibition of myeloperoxidase activity and oxidative stress in nonalcoholic steatohepatitisPubmed: 30387131
  • Bioactive Food Abates Metabolic and Synaptic Alterations by Modulation of Gut Microbiota in a Mouse Model of Alzheimer's DiseasePubmed: 30475761
  • Evaluation of the effect of Lactobacillus reuteri V3401 on biomarkers of inflammation, cardiovascular risk and liver steatosis in obese adults with metabolic …Pubmed: 30453950
  • Effects of fecal microbiota transplantation and joint application of probiotics on rats with alcoholic liver disease
  • Lactobacillus plantarum helps to suppress body weight gain, improve serum lipid profile and ameliorate low-grade inflammation in mice administered with glycerol …Doi: 10.1016/j.jff.2018.12.015
  • A combination of Pueraria lobata and Silybum marianum (L.) Gaerth protects against alcoholic liver disease in mice
  • Megasphaera elsdenii Lactate Degradation Pattern Shifts in Rumen Acidosis Models
  • Intestinal iNKT Cells Migrate to Liver and Contribute to Hepatocyte Apoptosis During Alcoholic Liver DiseasePubmed: 30817180
  • Influence of poly (I: C) variability on thermoregulation, immune responses and pregnancy outcomes in mouse models of maternal immune activation
  • Hepatic progenitor cell activation is induced by the depletion of the gut microbiome in micePubmed: 31094067
  • Additional Effect of Dietary Fiber in Patients with Type 2 Diabetes Mellitus Using Metformin and Sulfonylurea: An Open-Label, Pilot Trial
  • Lipopolysaccharide in systemic circulation induces activation of inflammatory response and oxidative stress in cardiorenal syndrome type 1Pubmed: 31006081
  • Intestinal injury and gut permeability in sickle cell diseasePubmed: 31146745
  • Glycerol Monolaurate‐Mediated Attenuation of Metabolic Syndrome is Associated with the Modulation of Gut Microbiota in High Fat diet‐fed MicePubmed: 31318165
  • Probiotics modulate the microbiota–gut–brain axis and improve memory deficits in aged SAMP8 mice
  • High-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights …
  • Lactobacillus reuteri V3401 Reduces Inflammatory Biomarkers and Modifies the Gastrointestinal Microbiome in Adults with Metabolic Syndrome: The PROSIR StudyPubmed: 31370223
  • The Combination of Ilexhainanoside D and Ilexsaponin A1 Reduces Liver Inflammation and Improves Intestinal Barrier Function in Mice with High-fat Diet-induced …
  • Effects of sinomenine in LPS‐associated diseases are related to inhibition of LBP, Mac‐1, and L‐selectin levelsPubmed: 31490576
  • Improvement of Lipoprotein Profile and Metabolic Endotoxemia by a Lifestyle Intervention That Modifies the Gut Microbiota in Subjects With Metabolic SyndromePubmed: 31451009
  • Depolymerized RG-I enriched pectin from citrus segment membrane modulates gut microbiota, increases SCFAs production, promotes the growth of Bifidobacterium …Pubmed: 31778135
  • Gut Dysbiosis with Minimal enteritis induced by High temperature and HumidityPubmed: 31822775
  • Ochratoxin A induces liver inflammation: involvement of intestinal microbiotaPubmed: 31779704
  • Compromised Ileal Mucus Barrier Due to Impaired Epithelial Homeostasis Caused by Notch1 Signaling in Cirrhotic RatsPubmed: 32144600
  • Liraglutide Attenuates Nonalcoholic Fatty Liver Disease by Modulating Gut Microbiota in Rats Administered a High-Fat DietPubmed: 32149099
  • Effect of resveratrol on intestinal tight junction proteins and the gut microbiome in high-fat diet-fed insulin resistant micePubmed: 32306796
  • The Gut Microbial Diversity of Newly Diagnosed Diabetics but Not of Prediabetics Is Significantly Different from That of Healthy NondiabeticsPubmed: 32234773
  • Disease-Specific Autoantibodies Induce Trained Immunity in RA Synovial Tissues and Its Gene Signature Correlates with the Response to Clinical TherapyPubmed: 33082707
  • The development of metabolic endotoxemia is dependent on the type of sweetener and the presence of saturated fat in the dietPubmed: 32804018
  • Aplysin Retards Pancreatic Necrosis and Inflammatory Responses in NOD Mice by Stabilizing Intestinal Barriers and Regulating Gut Microbial CompositionPubmed: 32801992
  • Gut-liver axis modulation in fructose-fed mice: a role for PPAR-alpha and linagliptinPubmed: 32698143
  • The colonic mucosa-associated microbiome in SIV infection: shift towards Bacteroidetes coincides with mucosal CD4+ T cell depletion and enterocyte damagePubmed: 32616803
  • Consumption of Cooked Black Beans Stimulates a Cluster of Some Clostridia Class Bacteria Decreasing Inflammatory Response and Improving Insulin SensitivityPubmed: 32340138
  • Pasteurella multocida Pm0442 Affects Virulence Gene Expression and Targets TLR2 to Induce Inflammatory ResponsesPubmed: 32922380
  • RAPID DIAGNOSIS OF PERITONITIS IN PERITONEAL DIALYSIS PATIENTS
  • Age-related cognitive decline is associated with microbiota-gut-brain axis disorders and neuroinflammation in mice33422597
  • IgG immunocomplexes drive the differentiation of a novel subset of osteoclasts independent of RANKL and inflammatory cytokines33651383
  • Bioengineered bacteria-derived outer membrane vesicles as a versatile antigen display platform for tumor vaccination via Plug-and-Display technology33824314
  • The Relationship between Low Serum Vitamin D Levels and Altered Intestinal Barrier Function in Patients with IBS Diarrhoea Undergoing a Long-Term Low-FODMAP33801020
  • Consumption of soybean or olive oil at recommended concentrations increased the intestinal microbiota diversity and insulin sensitivity and prevented fatty liver?¡­33915261
  • Somatization in patients with predominant diarrhoea irritable bowel syndrome: the role of the intestinal barrier function and integrity34022802
  • Inhibition of cyclooxygenase©\2 enhanced intestinal epithelial homeostasis via suppressing ¦Â©\catenin signalling pathway in experimental liver fibrosis34145945
  • Tissue transglutaminase is involved in the inflammatory processes of active chronic gastritis17539352
  • ¡°Adjusting Internal Organs and Dredging Channel¡± Electroacupuncture Ameliorates Insulin Resistance in Type 2 Diabetes Mellitus by Regulating the Intestinal?¡­34135611
  • Melatonin ameliorates ochratoxin A induced liver inflammation, oxidative stress and mitophagy in mice involving in intestinal microbiota and restoring the?¡­33359973
  • A Perfusion Bioreactor for Longitudinal Monitoring of Bioengineered Liver Constructs33494337
  • A rise in Proteobacteria is an indicator of gut-liver axis-mediated nonalcoholic fatty liver disease in high-fructose-fed adult mice34130208
  • Indole-3-propionic Acid-aggravated CCl4-induced Liver Fibrosis via the TGF-¦Â1/Smads Signaling Pathway
  • Gut barrier and microbiota changes with glycine and branched‐chain amino acid supplementation in chronic haemodialysis patients34535959
  • Intestinal pathophysiological abnormalities in steady state and after vaso‐occlusive crisis in murine sickle cell disease34632582
  • Inflammatory alveolar macrophage-derived microvesicles damage lung epithelial cells and induce lung injury34740720
  • Allicin Ameliorates Intestinal Barrier Damage via Microbiota-Regulated Short-Chain Fatty Acids-TLR4/MyD88/NF-κB Cascade Response in Acrylamide-Induced Rats34694121
  • Ligature induced periodontitis in rats causes gut dysbiosis leading to hepatic injury through SCD1/AMPK signalling pathway34813797
  • Lactobacillus acidophilus ameliorates obesity in mice through modulation of gut microbiota dysbiosis and intestinal permeability34896249
  • Psychological and Gastrointestinal Symptoms of Patients with Irritable Bowel Syndrome Undergoing a Low-FODMAP Diet: The Role of the Intestinal Barrier34371976
  • Lactobacillus casei relieves liver injury by regulating immunity and suppression of the enterogenic endotoxin‐induced inflammatory response in rats cotreated with …34646510
  • ALLICIN, A DIETARY TRPA1 AGONIST, PREVENTS HIGH FAT DIET-INDUCED DYSREGULATION OF GUT HORMONES AND ASSOCIATED COMPLICATIONS34705006
  • Indole-3-propionic Acid-aggravated CCl34966655
  • The Outer Membrane Vesicles of Salmonella enterica Serovar Typhimurium Activate Chicken Immune Cells through Lipopolysaccharides and Membrane ProteinsPubmed:35335663
  • Biomimic Trained Immunity‐MSCs Delivery Microcarriers for Acute Liver Failure RegenerationPubmed:35411651
  • Effects of the maternal gut microbiome and gut-placental axis on melatonin efficacy in alleviating cadmium-induced fetal growth restrictionPubmed:35487173
  • Gut dysbiosis promotes prostate cancer progression and docetaxel resistance via activating NF-κB-IL6-STAT3 axisPubmed:35710492
  • The Role of Aeromonas-Goblet Cell Interactions in Melatonin-Mediated Improvements in Sleep Deprivation-Induced ColitisPubmed:35355860
  • Co-interventions with Clostridium butyrate and soluble dietary fiber targeting gut microbiota improve MAFLD via the Acly/Nrf2/NFκ B signaling pathwayPubmed:35543143
  • Vascular Calcification and the Gut and Blood Microbiome in Chronic Kidney Disease Patients on Peritoneal Dialysis: A Pilot Study

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