Polyclonal Antibody to N-Terminal Pro-Atrial Natriuretic Peptide (NT-ProANP) 
NT-Pro-ANP; N-ANP
Overview
Properties
- Product No.PAA484Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryEndocrinologyCardiovascular biology
- SourcePolyclonal antibody preparation, Host Rabbit
- Ig Type IgG, Potency n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA484Hu01-Recombinant N-Terminal Pro-Atrial Natriuretic Peptide (NT-ProANP)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid, Concentration 0.35mg/ml
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Sample: Mouse Cardiac Muscle Tissue Primary Ab: 10µg/ml Rabbit Anti-Human NT-ProANP Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Mouse Cardiac Muscle Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human NT-ProANP Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Skeletal muscle Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human NT-ProANP Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant NT-ProANP, Human.
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Western Blot; Sample: Mouse Heart lysate
Primary Ab: 1μg/ml Rabbit Anti-Human NT-ProANP Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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ISO9001: 2008, ISO13485: 2003 Registered
Specifity
The antibody is a rabbit polyclonal antibody raised against NT-ProANP. It has been selected for its ability to recognize NT-ProANP in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat)Giveaways
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Citations
- Atrial natriuretic peptides detect cardiac dilatationBiblio.ugent: 3227757
- Prediction of postoperative atrial fibrillation with left atrial mechanical functions and NT‐pro ANP levels after coronary artery bypass surgery: A three‐dimensional …Pubmed:29457260
- Циррозы печени вирусной этиологии у детей
- Van Veterinary Journal
- Recombinant Soluble Corin Improves Cardiac Function in Mouse Models of Heart Failure33759549