Polyclonal Antibody to N-Terminal Pro-Atrial Natriuretic Peptide (NT-ProANP) 
NT-Pro-ANP; N-ANP
Overview
Properties
- Product No.PAA484Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsWB,IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryEndocrinologyCardiovascular biology
- SourcePolyclonal antibody preparation, Host Rabbit
- Ig Type IgG, Potency n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA484Ra01-Recombinant N-Terminal Pro-Atrial Natriuretic Peptide (NT-ProANP)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid, Concentration 500µg/mL
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Sample: Rat Cardiac Muscle Tissue; Primary Ab: 20µg/ml Rabbit Anti-Rat NT-ProANP Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Western Blot; Samples: Lane1: Rat Heart lysate; Lane2: Mouse Heart lysate; Primary Ab: 0.5µg/ml Rabbit Anti-Rat NT-ProANP Antibody Second Ab: 0.2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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ISO9001: 2008, ISO13485: 2003 Registered
Specifity
The antibody is a rabbit polyclonal antibody raised against NT-ProANP. It has been selected for its ability to recognize NT-ProANP in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.5-2µg/mL
Immunocytochemistry in formalin fixed cells: 5-20µg/mL
Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL
Immunohistochemistry in paraffin section: 5-20µg/mL
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Organism Species More: Mus musculus (Mouse)Giveaways
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Citations
- Atrial natriuretic peptides detect cardiac dilatationBiblio.ugent: 3227757
- Prediction of postoperative atrial fibrillation with left atrial mechanical functions and NT‐pro ANP levels after coronary artery bypass surgery: A three‐dimensional …Pubmed:29457260
- Циррозы печени вирусной этиологии у детей
- Van Veterinary Journal
- Recombinant Soluble Corin Improves Cardiac Function in Mouse Models of Heart Failure33759549