Polyclonal Antibody to Ferritin (FE)
Overview
Properties
- Product No.PAA518Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - Downloadn/a
- CategoryMetabolic pathwayTumor immunityHematology
- SourcePolyclonal antibody preparation, Host Rabbit
- Ig Type IgG, Potency n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid, Concentration 500µg/mL
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Specifity
The antibody is a rabbit polyclonal antibody raised against FE. It has been selected for its ability to recognize FE in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
Citations
- PGC-1α regulates hepatic hepcidin expression and iron homeostasis in response to inflammation.Pubmed: 23438894
- Redox-regulation of gelatinases during growth of cisplatin-sensitive and resistant Guerin carcinoma.PubMed: 25804229
- Host H68D Genotype Affects Tumor Growth in Mouse MelanomaOpen-Access: 1948-5956-1000353
- The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing micePubmed:26757699
- The Labile Iron Pool in Monocytes Reflects the Activity of the Atherosclerotic Process in Men with Chronic Cardiovascular Diseasepubmed:27782743
- 4 (th) Mediterranean Multidisciplinary Course on Iron Anemia April 29 (th)-30 (th) 2016, Madrid, Spain.pubmed:27615544
- Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activationpubmed:29147463
- sucrosomial® iron absorption studied by in vitro and ex-vivo modelspubmed:29055735
- In vitro assessment of bio-augmented minerals from peanut oil cakes fermented by Aspergillus oryzae through Caco-2 cells.pubmed:29051659
- Anemia and Iron Deficiency Related to Inflammation, Helicobacter pylori Infection and Adiposity in Reproductive-age Cuban Women10.1590/MEDICC.2017.1902030004
- The Role of Insulin Therapy in Correcting Hepcidin Levels in Patients with Type 2 Diabetes Mellitus.pubmed:28584599
- Preparation of lactose-iron complex and its cyto-toxicity, in-vitro digestion and bioaccessibility in Caco-2 cell model system10.1016/j.fbio.2017.10.001
- Long-Term Dexamethasone Exposure Down-Regulates Hepatic TFR1 and Reduces Liver Iron Concentration in Ratspubmed:28629118
- Physicochemical characterization of mineral (iron/zinc) bound caseinate and their mineral uptake in Caco-2 cellsPubmed:29622185
- Super-dosing phytase improves the growth performance of weaner pigs fed a low iron diet10.1016:j.anifeedsci.2018.01.012
- Iron homeostasis in pregnancy and spontaneous abortionPubmed: 30394565
- Assessment of iron deficiency anaemia and its risk factors among adults with chronic kidney disease in a tertiary hospital in Nigeria
- New thiazolidinones reduce iron overload in mouse models of hereditary hemochromatosis and β-thalassemiaPubmed: 30792208
- PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE
- Synthesis, characterization and cellular mineral absorption of nanoemulsions of Rhododendron arboreum flower extracts stabilized with gum arabic
- Transferrin plays a central role in coagulation balance by interacting with clotting factorsPubmed: 31811276
- Gum arabic capped copper nanoparticles: Synthesis, characterization, and applicationsPubmed: 31904465
- Improvement of Mineral Absorption and Nutritional Properties of Citrullus vulgaris Seeds Using Solid-State FermentationPubmed: 32255407
- Whey protein-iron or zinc complexation decreases pro-oxidant activity of iron and increases iron and zinc bioavailability
- Systemic iron deficiency does not affect the cardiac iron content and progression of heart failure34139233
- The mechanism of the imbalance between proliferation and ferroptosis in pulmonary artery smooth muscle cells based on the activation of SLC7A11Pubmed:35700835