Biotin-Linked Polyclonal Antibody to Collagen Type III (COL3)
Overview
Properties
- Product No.LAA176Hu71
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsWB; IHC; ICC.If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - Downloadn/a
- CategoryMetabolic pathwayCardiovascular biology
- SourceAntibody labeling
- Ig Type IgG, Potency n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelBiotin
- Original Antibody n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid, Concentration 500µg/mL
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Specifity
The antibody is a rabbit polyclonal antibody raised against COL3. It has been selected for its ability to recognize COL3 in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
Citations
- Triptolide inhibits extracellular matrix protein synthesis by suppressing the Smad2 but not the MAPK pathway in TGF-β1-stimulated NRK-49F cellsOxfordJournals: 3180
- Serial cytokine levels during wound healing in rabbit maxillary sinus mucosaPubMed: 19958244
- Modification of collagen formation by mesenchymal stem cells isolated from human adipose tissue in culture and after autotransplantation for abdominal hernia plastyPubmed: 24319714
- In vitro tenocyte metabolism in aging and oestrogen deficiencySpringer: Source
- Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jellyPubmed: 24739658
- Effect of nandrolone and/or whey protein on the soleus muscle and testis of adult male albino ratsCom:Source
- Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented mediaPubmed:Pmc4130592
- Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytesPubmed:25333747
- Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propriaPubmed:25545625
- KLF15 is an essential negative regulatory factor for the cardiac remodeling response to pressure overloadPubmed:25633973
- The skin remodeling in type 1 diabetes and insulin resistance animal modelsPubMed: 26047379
- The skin remodeling in type 1 diabetes and insulin resistance animal models.PubMed: 26047379
- Comparative Analysis of the Extracellular Matrix Composition in Proliferating and Involuted Infantile HemangiomasPubMed: 26430624
- Pituitary tumor transforming gene as a novel regulatory factor of liver fibrosisPubMed: 25936962
- SVVYGLR motif of the thrombin-cleaved N-terminal osteopontin fragment enhances the synthesis of collagen type III in myocardial fibrosisPubMed: 26112906
- KLF16 Is an Essential Negative Regulatory Factor for the Cardiac Remodeling Response to Pressure OverloadPubMed: 25633973
- Uighur medicine abnormal savda munzip (ASMq) suppresses expression of collagen and TGF-β1 with concomitant induce Smad7 in human hypertrophic scar …PubMed: 26309506
- IL-17A mediates inflammatory and tissue remodelling events in early human tendinopathyPubmed:27263531
- Інфаркт міокарда, ускладнений аневризмою лівого шлуночка: клінічні і структурно-функціональні особливості перебігу, діагностичні, терапевтичні та прогностичні аспектиbitstream:123456789
- Plasma LncRNA-ATB, a Potential Biomarker for Diagnosis of Patients with Coal Workers'Pneumoconiosis: A Case-Control Study.pubmed:27556453
- Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent andmitochondrial pathways in mammalian hepatic stellate cells.pubmed:27461108
- Genomic reprograming analysis of the Mesothelial to Mesenchymal Transition identifies biomarkers in peritoneal dialysis patients.pubmed:28327551
- Saikosaponin a Induces Apoptosis through Mitochondria-Dependent Pathway in Hepatic Stellate Cellspubmed:28231747
- Crystalline Silica Promotes Rat Fibrocyte Differentiation in Vitro, and Fibrocytes Participate in Silicosis in Vivopubmed:29081339
- Effects of the (Pro) renin Receptor on Cardiac Remodeling and Function in a Rat Alcoholic Cardiomyopathy Model via the PRR-ERK1/2-NOX4 Pathway
- Effect of proinflammatory cytokines on endometrial collagen and metallopeptidase expression during the course of equine endometrosisPubmed: 31265984
- Pantoea agglomerans chronic exposure induces epithelial-mesenchymal transition in human lung epithelial cells and mice lungsPubmed: 32146192
- Distinct differences in hypoxic responses between human oral mucosa and skin fibroblasts in a 3D collagen matrixPubmed: 32588253
- Tissue-Engineered Decellularized Allografts for Anterior Cruciate Ligament Reconstruction
- Enhanced cell survival and therapeutic benefits of IL-10-expressing multipotent mesenchymal stromal cells for muscular dystrophy33541428
- Cell sheet technology: Influence of culture conditions on in vitro©\cultivated corneal stromal tissue for regenerative therapies of the ocular surface33538123
- Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment¡ªIn vivo studies33999928
- Uncarboxylated osteocalcin alleviates the inhibitory effect of high glucose on osteogenic differentiation of mouse bone marrow¨Cderived mesenchymal stem?¡33906607
- Early diagnosis of myocardial fibrosis in patients with epicardial obesity
- Manuscript title Microparticles Containing MiR-625-5p In Coronary Artery Reduce Myocardial Fibrosis After Myocardial Infarction