Monoclonal Antibody to Alpha-Melanocyte Stimulating Hormone (aMSH) Monoclonal antibody

α-MSH; Intermedins; Alpha-Melanotropin, Alpha-Melanocortin; Alpha-Intermedin

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG1 Kappa, Clone Number C4
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen CPA239Mi21-OVA Conjugated Alpha-Melanocyte Stimulating Hormone (aMSH)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Alpha-Melanocyte Stimulating Hormone (aMSH) Packages (Simulation)
  • Monoclonal Antibody to Alpha-Melanocyte Stimulating Hormone (aMSH) Packages (Simulation)
  • Monoclonal Antibody to Alpha-Melanocyte Stimulating Hormone (aMSH) DAB staining on IHC-P;
    Sample: Porcine Pituitary Tissue
    Primary Ab: 30µg/ml Mouse Anti-Multi-species aMSH Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against aMSH. It has been selected for its ability to recognize aMSH in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Generation of a human bone marrow-derived mesenchymal stem cell line expressing and secreting high levels of bioactive α-melanocyte-stimulating hormone.Pubmed: 23341471
  • Pharmacological induction of skin pigmentation unveils the neuroendocrine circuit regulated by lightPubMed: 26582755
  • Intermedin Restores Hyperhomocysteinemia-induced Macrophage Polarization and Improves Insulin Resistance in MicePubmed:27080257
  • Pharmacological induction of skin pigmentation unveils the neuroendocrine circuit reCavia (Guinea pig )lated by lightPubmed:26582755
  • Endocrine disruptors induce perturbations in endoplasmic reticulum and mitochondria of human pluripotent stem cell derivatives.pubmed:28794470
  • Super-Obese Patient-Derived iPSC Hypothalamic Neurons Exhibit Obesogenic Signatures and Hormone ResponsesPubmed:29681516
  • Epigenetic alterations of the POMC promoter in tobacco dependencePubmed:29871818
  • Mechanisms of sustained long-term weight loss after RYGB: α-MSH is a key factorPubmed:29685637
  • Alcohol Withdrawal and Proopiomelanocortin Neuropeptides in an Animal Model of Alcohol DependencePubmed: 31117084
  • Intermedin alleviates the inflammatory response and stabilizes the endothelial barrier in LPS-induced ARDS through the PI3K/Akt/eNOS signaling pathwayPubmed: 32892076
  • Serum alpha-melanocyte-stimulating hormone (a-MSH), brain-derived neurotrophic factor (BDNF), and agouti-related protein (AGRP) levels in children with Prader …Pubmed:35098494

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