Monoclonal Antibody to Heat Shock Protein 70 (HSP70)
DnaK; 70 Kilodalton Heat Shock Proteins
Overview
Properties
- Product No.MAA873Hu21
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryTumor immunityInfection immunityDevelopmental science
- SourceMonoclonal antibody preparation, Host Mouse
- Ig Isotype IgG2b Kappa, Clone Number C7
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid, Concentration 1mg/ml
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Specifity
The antibody is a mouse monoclonal antibody raised against HSP70. It has been selected for its ability to recognize HSP70 in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
Citations
- Proteomic analysis reveals significant elevation of heat shock protein 70 in patients with chronic heart failure due to arrhythmogenic right ventricular cardiomyopathySpringerLink: b8738j3571287425
- Neuroinflammation in autism spectrum disordersPubMed: PMC3549857
- Luteal serum BDNF and HSP70 levels in women with premenstrual dysphoric disorderPubmed: 23455589
- Concentraciones de proteínas de estrés térmico, L-arginina y dimetilarginina asimétrica en pacientes con síndrome de apnea obstructiva del suePubMed: 26055465
- Heat Shock Proteins,PubMed: 26055465
- The effect of heat stress on gene expression and synthesis of heat‐shock and milk proteins in bovine mammary epithelial cellsPubMed: 26467738
- L-Alanyl-Glutamine Attenuates Oxidative Stress in Liver Transplantation PatientsPubMed: 26518955
- Long-Term Effects of Induced Hypothermia on Local and Systemic Inflammation-Results from a Porcine Long-Term Trauma ModelPubmed:27144532
- Elevated interictal serum HSP-70 levels as an indicator of neurodegeneration for chronic migrainemnstemps:33-1429777310
- STUDY THE RELATIONSHIP BETWEEN SELENIUM AND HEAT SHOCK PROTEINS UNDER HEAT STRESS FOR LOCAL SINAI CHICKENS STRAINabstract:3d115259031
- Physiological consequences for rainbow trout (Oncorhynchus mykiss) of short-term exposure to increased suspended solid loadS0144860916301935
- Prokaryotic expression of chicken interferon-γ fusion protein and its effect on expression ofpoultry heat shock protein 70 under heat stress.pubmed:27753175
- High-dose testosterone enanthate supplementation boosts oxidative stress, but exerts little effect on the antioxidant barrier in sedentary adolescent male rat liver.pubmed:28531762
- SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death.pubmed:28122311
- Concentrations of SP-A and HSP70 are associated with polarization of macrophages in pleural effusions of non-small cell lung cancerpubmed:29111316
- EFFECT OF DIETARY SOURCE OF SELENIUM ON PRODUCTIVE AND REPRODUCTIVE PERFORMANCE OF SINAI LAYING HENS UNDER HEAT STRESS CONDITIONS.125788660
- SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from deathDOI:10.1016/j.biopha.2017.01.087
- IMPACT OF CHROMIUM PICOLINATE SUPPLEMENTATION ON PRODUCTIVE PERFORMANCE, IMMUNE RESPONSE AND HEAT SHOCK PROTEINS OF BROILER CHICKENS UNDER HEAT-STRESS CONDITION.ISSN: 1110-5623
- Physiological consequences of chronic exposure of rainbow trout (Oncorhynchus mykiss) to suspended solid load in recirculating aquaculture systems10.1016/j.aquaculture.2017.11.030
- Detection of 70 kDa heat shock protein in the saliva of dairy cows.pubmed:28625184
- Reducing rumen starch fermentation of wheat with three percent sodium hydroxide has the potential to ameliorate the effect of heat stress in grain-fed wetherspubmed:29293755
- Ectopic expression of Miro 1 ameliorates seizures and inhibits hippocampal neurodegeneration in a mouse model of pilocarpine epilepsyPubmed:29365285
- THE EFFECT OF CHROMIUM PICOLINATE SUPPLEMENTATION ON IMMUNE RESPONSE AND HEAT SHOCK PROTEINS OF LAYING HENS UNDER HEAT …article_5570_5c06907ac09a7724a58d2b796fe91aba.pdf
- EFFECT OF MITIGATION OF HEAT STRESS BY EARLY HEATE ACCLIMATION AND GLUTAMINE INJECTION ON SOME PHYSIOLOGICAL MEASUREMENTS …:
- Signature-oriented investigation of the efficacy of multicomponent drugs against heart failurePubmed: 30230922
- Maged Mohamed Abd-Elhakim Barakat, Noha Ahmed El-Boghdady, Ebtehal Kamal Eldin Farrag, Ataa Abdelhalim Said and Sylvia Edward Shaker
- Effective Visualization and Easy Tracking of Extracellular Vesicles in Glioma Cells
- Protective and Curative Effects of Bombax ceiba Flower and Ziziphus spina christi Fruit Extracts on Gastric Ulcer
- PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE
- Possible prophylactic effect of omega-3 fatty acids on cadmium-induced neurotoxicity in rats' brainsPubmed: 31468353
- Oxidative stress mediated apoptotic potential of mefloquine on experimental trichinellosisPubmed: 33221280
- Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat StressPubmed: 32685488
- EFFECTS OF HEAT STRESS ON PHYSIOLOGICAL PARAMETERS AND SERUM CONCENTRATION OF HSP70 IN INDIGENOUS BREEDS OF SHEEP IN NIGERIA
- Effect of organic acid blend and prebiotic on growth performance gut histomorphometry, tibia bone characteristics, reactive oxygen species, microbial count and …
- Concurrent high-intensity aerobic and resistance exercise modulates systemic release of alarmins (HMGB1, S100A8/A9, HSP70) and inflammatory …
- Physiological impacts of suspended solids in recirculating aquaculture
- Evaluation of heat shock protein 70 and toll-like receptor 4 expression in gingival crevicular fluid in response to orthodontic forces34091792
- Effects of L-proline on cellular responses of hen erythrocytes subjected to thermal stress33627283
- The Restorative Effect of Gallic Acid on the Experimental Sciatic Nerve Damage Model34376039
- Neuroprotective effect of sodium alginate against chromium-induced brain damage in ratsPubmed:35421180
- Identification of marker proteins in preterm and term birth: Relationship of rankl and HSP70 in the cord blood