Monoclonal Antibody to Histone H3 (H3) 
Overview
Properties
- Product No.MAA285Mi22
- Organism SpeciesHomo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Canis familiaris; Canine (Dog), Bos taurus; Bovine (Cattle) Same name, Different species.
- ApplicationsWB
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryDevelopmental science
- SourceMonoclonal antibody preparation, Host Mouse
- Ig Isotype IgG2b Kappa, Clone Number C6
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPA285Mi01-Recombinant Histone H3 (H3)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid, Concentration 1mg/mL
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Packages (Simulation)
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Packages (Simulation)
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Western Blot; Sample: Lane1: Human Placenta lysate; Lane2: U2OS cell lysate; Lane3: Porcine Liver lysate; Lane4: Porcine Cerebrum lysate Primary Ab: 0.2μg/ml Mouse Anti-Multi-species H3 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: Rat Cerebrum lysate; Lane2: Mouse Cerebrum lysate; Lane3: Bovine Cerebrum lysate; Lane4: Canine Cerebrum lysate
Primary Ab: 0.2µg/ml Mouse Anti-Multi-species H3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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ISO9001: 2008, ISO13485: 2003 Registered
Specifity
The antibody is a mouse monoclonal antibody raised against H3. It has been selected for its ability to recognize H3 in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.01-2µg/mL;
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Organism Species More: Sus scrofa; Porcine (Pig)Giveaways
Increment services
Citations
- A Novel Anti-Histone H1 Monoclonal Antibody, SSV Monoclonal Antibody, Improves Lung Injury and Survival in a Mouse Model of Lipopolysaccharide-Induced Sepsis-Like SyndromePubMed: 25649890
- Proteomic analysis reveals USP7 as a novel regulator of palmitic acid-induced hepatocellular carcinoma cell deathPubmed:35732625