Monoclonal Antibody to Interleukin 35 (IL35) Homo sapiens (Human) Monoclonal antibody

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  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG, Clone Number B5
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPC008Hu01-Recombinant Interleukin 35 (IL35)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Interleukin 35 (IL35) Packages (Simulation)
  • Monoclonal Antibody to Interleukin 35 (IL35) Packages (Simulation)
  • MAC008Hu21.jpg Figure.Western Blot; Sample: Recombinant IL35, Human.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered


The antibody is a mouse monoclonal antibody raised against IL35. It has been selected for its ability to recognize IL35 in immunohistochemical staining and western blotting.


Western blotting: 0.5-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-30ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
Immunohistochemistry in paraffin section: 5-30ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.


Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.


The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.


  • Aberrant expression of Treg-associated cytokine IL-35 along with IL-10 and TGF-β in acute myeloid leukemia.PubMed: 22783403
  • Plasma IL-17, IL-35, interferon-γ, SOCS3 and TGF-β levels in pregnant women with preeclampsia, and their relation with severity of diseasePubmed: 24175856
  • What is the impact of Th1/Th2 ratio, SOCS3, IL17, and IL35 levels in unexplained infertility?Pubmed: 24368037
  • The imbalanced profile and clinical significance of T helper associated cytokines in bone marrow microenvironment of the patients with acute myeloid leukemiaScienceDirect: S0198885913005776
  • Gene Expression of Subunits of the IL-12 Family Cytokines in moDCs Derived In Vitro from the Cord Blood of Children of Healthy and Allergic MothersPubmed:24785110
  • Function of interleukin?17 and ?35 in the blood of patients with hepatitis B?related liver cirrhosisPubmed:25323532
  • The regular distribution and expression pattern of immunosuppressive cytokine IL-35 in mouse uterus during early pregnancyPubmed:25611266
  • Wogonin Inhibits Tumor-derived Regulatory Molecules by Suppressing STAT3 Signaling to Promote Tumor ImmunityPubMed: 25962106
  • Interleukin 35 Synovial Fluid Levels Are Associated with Disease Activity of Rheumatoid ArthritisPubMed: 26204444
  • Interleukin-35 is upregulated in systemic sclerosis and its serum levels are associated with early diseasePubMed: 26231346
  • Anti-inflammatory effects of interleukin-35 in acquired aplastic anemiaPubMed: 26282938
  • Interleukin-35 Inhibits Endothelial Cell Activation by Suppressing MAPK-AP-1 PathwayPubMed: 26085094
  • Interleukin 35 may contribute to the loss of immunological self‐tolerance in patients with primary immune thrombocytopeniaPubMed: 25640666
  • New insights into immune mechanisms underlying response to Rituximab in patients with membranous nephropathy: A prospective study and a review of the literaturePubmed:26876383
  • Increased plasma concentrations of interleukin 35 in patients with coronary artery diseaseJournal:-19
  • The clinical utility of serum IL-35 in patients with polymyositis and dermatomyositispubmed:27502600
  • Enhanced LPS-induced activation of IL-27 signalling in sarcoidosispubmed:27492538
  • Effects of an intravitreal injection of interleukin-35-expressing plasmid on pro-inflammatory andanti-inflammatory cytokines.pubmed:27460435
  • IL-35在牙周炎及口腔扁平苔藓中的表达及相关性分析QK:98604X

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