Monoclonal Antibody to Neurokinin A (NKA) 
NK-A; SK; Substance K; Neuropeptide K; Neurokinin K; Neuromedin L; Neuropeptide gamma
Overview
Properties
- Product No.MAA428Hu21
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsWB,IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategorySignal transductionNeuro science
- SourceMonoclonal antibody preparation, Host Mouse
- Ig Isotype IgG2b Kappa, Clone Number C1
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen CPA428Hu11-BSA Conjugated Neurokinin A (NKA)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid, Concentration 1mg/ml
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Sample: Human Cerebrum Tissue; Primary Ab: 20µg/ml Mouse Anti-Human NKA Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Prostate Tissue;
Primary Ab: 20µg/ml Mouse Anti-Human NKA Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Secondary antibody only control: Used PBS instead of primary antibody , Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Prostate Tissue
Primary Ab: 20µg/ml Mouse Anti-Human NKA Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: Rat Cerebrum lysate; Lane2: Porcine Cerebrum lysate; Lane3: Porcine Liver lysate; Lane4: 293T cell lysate Primary Ab: 5µg/ml Mouse Anti-Multi-species NKA Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19) Selected
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ISO9001: 2008, ISO13485: 2003 Registered
Specifity
The antibody is a mouse monoclonal antibody raised against NKA. It has been selected for its ability to recognize NKA in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.5-5µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Organism Species More: Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)
