Polyclonal Antibody to Angiotensin II (AngII) Homo sapiens (Human) Polyclonal antibody

Ang-II; Angiotensin-2

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen CPA005Mi21-OVA Conjugated Angiotensin II (AngII)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Angiotensin II (AngII) Packages (Simulation)
  • Polyclonal Antibody to Angiotensin II (AngII) Packages (Simulation)
  • PAA005Mi01.jpg Western Blot; Sample: HepG2 cell lysate Primary Ab: 1μg/ml Rabbit Anti-Multi-species AngII Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Angiotensin II (AngII) Western Blot; Sample: LO2 cell lysate
    Primary Ab: 0.3µg/ml Rabbit Anti-Multi-species AngII Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against AngII. It has been selected for its ability to recognize AngII in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Angiotensin-Converting Enzyme 2 Overexpression Remarkably Ameliorated Glomerular Injury in a Rat Model of Diabetic Nephropathy: A Comparison with ACE InhibitionMolMed: 10_11_liu
  • Mesangial medium with IgA1 from IgA nephropathy inhibits nephrin expression in mouse podocytesWiley: source
  • Evidence for the complementary and synergistic effects of the three-alkaloid combination regimen containing berberine, hypaconitine and skimmianine on the ulcerative colitis rats induced by trinitrobenzene-sulfonic acidScienceDirect: S0188440910001049
  • Effect of aggregated immunoglobulin A1 from immunoglobulin A nephropathy patients on nephrin expression in podocytesWiley: source
  • Angiotensin II and Angiotensin-(1-7) in Paraventricular Nucleus Modulate Cardiac Sympathetic Afferent Reflex in Renovascular Hypertensive RatsPubMed: PMC3527547
  • Heterogeneous Downregulation of Angiotensin II AT1-A and AT1-B Receptors in Arterioles in STZ-Induced Diabetic Rat KidneysHindawi: 947506
  • Biglcan is beneficial in angiotensin II induced heart failure by preventing cardiac inflammation and remodeling improving LV function and mortality by preventing transdifferentiation of myofibroblastsOxfordjournals: Source
  • SOD1 gene transfer into paraventricular nucleus attenuates hypertension and sympathetic activity in spontaneously hypertensive ratsPubmed: 23114721
  • Angiotensin II and vasopressin are involved in the defense system against anaphylactic hypotension in anesthetized ratsPubmed:24650734
  • Increased LDL electronegativity in chronic kidney disease disrupts calcium homeostasis resulting in cardiac dysfunctionPubmed:25871829
  • High glucose induces activation of the local renin?angiotensin system in glomerular endothelial cellsPubmed:24337709
  • Angiotensin II promotes the progression of human gastric cancerPubmed:24424956
  • Intermedin in the Paraventricular Nucleus Attenuates Cardiac Sympathetic Afferent Reflex in Chronic Heart Failure RatsPubmed:24709972
  • Cathepsin G deficiency reduces periaortic calcium chloride injury-induced abdominal aortic aneurysms in mice Pubmed:25037606
  • Cathepsin G activity lowers plasma LDL and reduces atherosclerosisPubmed:25092171
  • Superoxide anions in the paraventricular nucleus mediate cardiac sympathetic afferent reflex in insulin resistance ratsPubmed:25307720
  • Administration of exogenous 1,25(OH)2D3 normalizes overactivation of the central renin-angiotensin system in 1α(OH)ase knockout micePubmed:25576706
  • A background Ca2+ entry pathway mediated by TRPC1/TRPC4 is critical for development of pathological cardiac remodellingPubMed: 26069213
  • Cardioprotective effects of baicalein on heart failure via modulation of Ca2PubMed: 26706290
  • Renal Sympathetic Denervation Improves Cardiac DysfunctionPubMed: 25804096
  • Effects of Rubus occidentalis extract on blood pressure in subjects with prehypertension: randomized, double-blinded, placebo controlled clinical trialPubMed: 26740254
  • Angiotensin-(1–7) enhances the effects of angiotensin II on the cardiac sympathetic afferent reflex and sympathetic activity in rostral ventrolateral medulla in renovascular hypertensive ratsPubMed: 26428223
  • Up ReCavia (Guinea pig )lation of cystathione γ lyase and Hydrogen Sulphide in the Myocardium Inhibits the Progression of Isoproterenol–Caffeine Induced Left Ventricular Hypertrophy in Wistar Kyoto RatsPubmed:26963622
  • Effects of Rubus occidentalis extract on blood pressure in patients with prehypertension: Randomized, double-blinded, placebo-controlled clinical trialPubmed:26740254
  • Involvement of Angiotensin II Type 1 and 2 Receptors in Gelatinase ReCavia (Guinea pig )lation in Human Carotid Atheroma in vitroPubmed:26947595
  • A Therapeutic Insight of Niacin and Coenzyme Q10 Against Diabetic Encephalopathy in RatsPubmed:26867655
  • Cardioprotective effects of baicalein on heart failure via modulation of Ca(2+) handling proteins in vivo and in vitroPubmed:26706290
  • Paradoxical Sleep Deprivation Causes Cardiac Dysfunction and the Impairment Is Attenuated by Resistance Training.pubmed:27880816
  • Epigallocatechin-3-gallate Attenuates Renal Damage by Suppressing Oxidative Stress in Diabetic db/db Micepubmed:27698952
  • Angiotensin type 2 receptor null mice express reduced levels of renal angiotensin converting enzyme-2/angiotensin (1-7)/Mas receptor and exhibit greater high-fat diet-induced kidney injury1470320316661871.abstract
  • Pleiotropic effects of survivin in vascular endothelial cells.pubmed:27373518
  • Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated withActivation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension.pubmed:27391973
  • β-Aminoisobutyric acid ameliorates the renal fibrosis in mouse obstructed kidneys via inhibitionof renal fibroblast activation and fibrosis.pubmed:28433566
  • Ginsenoside Rb1 improves cardiac function and remodeling in heart failure.pubmed:28367863
  • Effects of renal denervation on monocrotaline induced pulmonary remodelingpubmed:28187460
  • The Renin-Angiotensin System Regulates Neurodegeneration in a Mouse Model of Optic Neuritispubmed:28919108
  • Enhanced heart failure, mortality and renin activation in female mice with experimental dilated cardiomyopathypubmed:29240788
  • Hydrogen Sulfide Attenuates Atherosclerosis in a Partially Ligated Carotid Artery Mouse model via Regulating Angiotensin Converting Enzyme 2 Expressionpubmed:29066981
  • Brain Renin–Angiotensin System Blockade Attenuates Methamphetamine-Induced Hyperlocomotion and NeurotoxicityPubmed:29464572
  • ALDH2 protects against alcoholic cardiomyopathy through a mechanism involving the p38 MAPK/CREB pathway and local renin-angiotensin system inhibition in …Pubmed:29506687
  • Angiotensin II Type 1a Receptors in the Subfornical Organ Modulate Neuroinflammation in the Hypothalamic Paraventricular Nucleus in Heart Failure RatsPubmed:29684507
  • Role of (Pro) Renin Receptor in Albumin Overload-Induced Nephropathy in RatsPubmed:29846109
  • Mesenchymal stem cell–derived microvesicles alleviate pulmonary arterial hypertension by regulating renin-angiotensin systemPubmed:29752040
  • Acute Biochemical, Cardiovascular, and Autonomic Response to Hyperbaric (4 atm) Exposure in Healthy SubjectsPubmed:29977313
  • Renovascular hypertension increases serum TNF and CX3CL1 in experimental Trypanosoma cruzi infectionPubmed:29590257
  • Sex Differences in the Central and Peripheral Manifestations of Ischemia-induced Heart Failure in RatsPubmed: 30289294
  • Long-term exposure to crotonaldehyde causes heart and kidney dysfunction through induction of inflammatory and oxidative damage in male Wistar ratsPubmed: 30426860
  • Detection of Angiotensin II and AT1 Receptor Concentrations in Keloid and Hypertrophic Scar.
  • Serum microRNA-221 as a biomarker for diabetic retinopathy in patients associated with type 2 diabetesPubmed: 30588418
  • ACE Gene Plays a Key Role in Reducing Blood Pressure in The Hyperintensive Elderly After Resistance TrainingPubmed: 30908459
  • T lymphocyte-based renin angiotensin system in obesity
  • Cerebrospinal Fluid Changes in the Renin-Angiotensin System in Alzheimer's DiseasePubmed: 31594235
  • Seminal levels of angiotensin II and angiotensin II type 2 receptor expression on spermatozoa in varicocele patients: Relation to fertility statusPubmed: 31989676
  • BPA disrupts 17‑estradiol‑mediated hepatic protection against ischemia/reperfusion injury in rat liver by upregulating the Ang II/AT1R signaling pathwayPubmed: 32319667
  • The sympathetic transmitter norepinephrine inhibits VSMC proliferation induced by TGFβ by suppressing the expression of the TGFβ receptor ALK5 in aorta …Pubmed: 32319652
  • Predator Scent-Induced Sensitization of Hypertension and Anxiety-like BehaviorsPubmed: 33201417
  • Effect of repetitive potassium iodide on thyroid and cardiovascular functions in elderly ratsPubmed: 33024842
  • Angiotensin II enhances the proliferation of Natural Killer/T-cell lymphoma cells via activating PI3K/Akt signaling pathwayPubmed: 32969473
  • A (pro) renin receptor decoy peptide PRO20 protects against adriamycin-induced nephropathy by targeting intrarenal renin-angiotensin systemPubmed: 32865014
  • THE ROLE OF HIGH FOLLICULAR LEVELS OF ANGIOTENSIN II AND VASCULAR ENDOTHELIAL GROWTH FACTOR IN ANTICIPATING THE …Pubmed: 32685035
  • Proteomics and informatics for understanding phases and identifying biomarkers in COVID-19 diseasePubmed: 32657586
  • Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in ratsPubmed: 32616735
  • Semen Brassicae reduces thoracic aortic remodeling, inflammation, and oxidative damage in spontaneously hypertensive ratsPubmed: 32570115
  • Hyperinsulinemia rather than insulin resistance itself induces blood pressure elevation in high fat diet-fed ratsPubmed: 32349626
  • Tianma Gouteng Decoction Exerts Cardiovascular Protection by Upregulating OPG and TRAIL in Spontaneously Hypertensive RatsPubmed: 33133215
  • Sinapic Acid Attenuates Cardiovascular Disorders in Rats by Modulating Reactive Oxygen Species and Angiotensin Receptor ExpressionPubmed: 32765804
  • Gut Dysbiosis Contribute to High Fructose Induced Salt Sensitive Hypertension In Sprague-Dawley Rats
  • Dikkat Eksikliği Hiperaktivite Bozukluğu Tanısı Olan Anjiyotensin I, II, III, Alamandin peptid, Anjiyotensin Dönüştürücü Enzim (ACE), NADPH Oksidaz Enzim Serum …
  • Low-load resistance training with blood flow restriction prevent renal function decline: The role of the redox balance, angiotensin 1-7 and vasopressin33340514
  • The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice33303990
  • The Effects of Renal Nerve Denervation on Blood Pressure and Target Organs in Different Hypertensive Rat Models33884205
  • (Pro) renin receptor antagonist PRO20 attenuates nephrectomy©\induced nephropathy in rats via inhibition of intrarenal RAS and Wnt/¦Â©\catenin signaling34057312
  • Renovascular effects of inorganic nitrate following ischemia-reperfusion of the kidney33360353
  • Paeonol Protects Against Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson's Disease33995926
  • Ang-(1-7) Protects Skeletal Muscle Function in Aged Mice
  • Lycium barbarum polysaccharides attenuate cardiovascular oxidative stress injury by enhancing the Keap1/Nrf2 signaling pathway in exhaustive exercise rats34278476
  • Ang-(1–7) protects skeletal muscle function in aged mice34548056
  • Maternal Angiotensin II–Induced Hypertension Sensitizes Postweaning High‐Fat Diet–Elicited Hypertensive Response Through Increased Brain Reactivity in Rat …34482712
  • Tianma Gouteng Decoction regulates oxidative stress and inflammation in AngII-induced hypertensive mice via transcription factor EB to exert anti …34736077
  • Loss of the Protective Effect of Estrogen Contributes to Maternal Gestational Hypertension‐Induced Hypertensive Response Sensitization Elicited by Postweaning …Pubmed:35014859
  • Dysregulation of ACE-1 in normal aging and the early stages of Alzheimer's diseasePubmed:35396835
  • Corynoline protects ang II-induced hypertensive heart failure by increasing PPARα and Inhibiting NF-κB pathwayPubmed:35658238

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