Polyclonal Antibody to Complement Component 5a (C5a) 
Overview
Properties
- Product No.PAA388Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryInfection immunityImmune moleculeAutoimmunity
- SourcePolyclonal antibody preparation, Host Rabbit
- Ig Type IgG, Potency n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA388Ra01-Recombinant Complement Component 5a (C5a)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid, Concentration 0.45mg/mL
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Rat Stomach Tissue; Primary Ab: 20μg/ml Rabbit Anti-Rat C5a Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Rat Liver Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Rat C5a Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant C5a, Rat.
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Western Blot; Samples: Lane1: Rat Serum; Lane2: Rat Plasma;
Primary Ab: 0.1µg/ml Rabbit Anti-Rat C5a Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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ISO9001: 2008, ISO13485: 2003 Registered
Specifity
The antibody is a rabbit polyclonal antibody raised against C5a. It has been selected for its ability to recognize C5a in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
Citations
- Cathepsin D is released after severe tissue trauma in vivo and is capable of generating C5a in vitroScienceDirect: S0161589011008297
- Elevated complement factor C5a in maternal and umbilical cord plasma in preeclampsiaPubMed: 23415845
- Complement component 5 contributes to poor disease outcome in humans and mice with pneumococcal meningitisPubMed: PMC3195471
- Efficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis modelPubmed: 23645587
- Conversion from external fixator to intramedullary nail causes a second hit and impairs fracture healing in a severe trauma modelPubmed: 23070742
- Therapeutic effects of sesame oil on monosodium urate crystal-induced acute inflammatory response in ratsPubmed: 24353977
- Evaluation of the Markers of Inflammation in the Umbilical Cord Blood of Newborns of Mothers with ThrombophiliaPubmed:25209155
- Complement Split Products C3a/C5a and Receptors: Are They Regulated by Circulating Angiotensin II Type 1 Receptor Autoantibody in Severe Preeclampsia?PubMed: 26485247
- Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitisPubmed:27235358
- Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgeryPubmed:26802602
- Dosagem de frações ativadas do sistema complemento em empiema induzido em ratos10183
- Cadmium-induced immune abnormality is a key pathogenic event in human and rat models of preeclampsiapubmed:27511439
- Complement C5a/C5aR pathway potentiates the pathogenesis Q5 of gastric cancer by down-regulating p21 expressionpubmed:29031586
- Serum complement factor 5a levels are associated with nonalcoholic fatty liver disease in obese childrenDOI: 10.1111/apa.14106
- Splenectomy modulates early immuno-inflammatory responses to trauma-hemorrhage and protects mice against secondary sepsisPubmed: 30291296
- The Beneficial Effects of Lactobacillus plantarum PS128 on High-Intensity, Exercise-Induced Oxidative Stress, Inflammation, and Performance in TriathletesPubmed: 30736479
- Intrathecal complement activation by the classical pathway in tick-borne encephalitisPubmed: 30850976
- Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis modelPubmed: 31247004
- C5a/C5aR Pathway is Critical for the Pathogenesis of PsoriasisPubmed: 31447855
- Novel evidence that an alternative complement cascade pathway is involved in optimal mobilization of hematopoietic stem/progenitor cells in Nlrp3 …
- C5aR deficiency attenuates the breast cancer development via the p38/p21 axisPubmed: 32669478
- A Novel Evidence That Mannan Binding Lectin (MBL) Pathway of Complement Cascade Activation is Involved in Homing and Engraftment of Hematopoietic Stem …Pubmed: 32406006
- Modulation of C5a-C5aR interactions against murine mammary cancer cell line
- C‐reactive protein inhibits C3a/C3aR‐dependent podocyte autophagy in favor of diabetic kidney diseasePubmed:35503088