Polyclonal Antibody to Laminin (LN) Mus musculus (Mouse) Polyclonal antibody

LAM

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen NPA082Mu01-Native Laminin (LN)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Laminin (LN) Packages (Simulation)
  • Polyclonal Antibody to Laminin (LN) Packages (Simulation)
  • PAA082Mu01.jpg Western Blot; Sample: Mouse Placenta lysate Primary Ab: 0.2µg/ml Rabbit Anti-Mouse LN Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against LN. It has been selected for its ability to recognize LN in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jellyPubmed: 24739658
  • Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented mediaPubmed:Pmc4130592
  • Augmented expression of urokinase plasminogen activator and extracellular matrix proteins associates with multiple myeloma progressionPubmed:24807734
  • Investigation?of?diagnostic?potentials?of?nine?different?biomarkers?in?endometriosis.Pubmed:24813083
  • Comparative Analysis of the Extracellular Matrix Composition in Proliferating and Involuted Infantile HemangiomasPubMed: 26430624
  • Modified Citrus Pectin stops progression of liver fibrosis by inhibiting galactin-3 and inducing apoptosis of stellate cellsDoi: Abs
  • Clinical significance of serum laminin and type‑IV collagen levels in cutaneous melanoma patientsPubmed:27330797
  • Key Matrix Proteins Within the Pancreatic Islet Basement Membrane Are Differentially Digested During Human Islet Isolation.pubmed:27456745
  • Modulatory Effects of Chemoradiation on Angiogenic Factors and Laminin in Cervical Cancer: Link with Treatment Responsepubmed:29172262
  • Coordination Among Lipid Droplets, Peroxisomes and Mitochondria Regulates Energy Expenditure Through the CIDE-ATGL-PPARα Pathway in AdipocytesPubmed:29986925
  • Evaluation of antifibrotic effects of coffee and cocoa extracts in rats with thioacetamide-induced fibrosis10.1007:s00217-018-3119-z
  • High amplitude stretching of ATII cells and fibroblasts results in profibrotic effectsPubmed: 31290711
  • Fabrication and Evaluation of a Xenogeneic Decellularized Nerve-Derived Material: Preclinical Studies of a New Strategy for Nerve RepairPubmed: 31758411
  • Untersuchungen und semiquantitative Symmetrie-Analysen zur Extrazellulärmatrix von Kalbs-Stimmlippen
  • CLINICAL SIGNIFICANCE OF LAMININ AND ELASTINE LEVELS IN CHILDREN WITH UNDIFFERENTIATED CONNECTIVE TISSUE DISEASE

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