Polyclonal Antibody to Secretory Immunoglobulin A (sIgA) Mus musculus (Mouse) Polyclonal antibody

S-IgA

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/mL
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  • Polyclonal Antibody to Secretory Immunoglobulin A (sIgA) Packages (Simulation)
  • Polyclonal Antibody to Secretory Immunoglobulin A (sIgA) Packages (Simulation)
  • Polyclonal Antibody to Secretory Immunoglobulin A (sIgA) DAB staining on IHC-P;
    Sample: Rat Colon Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Mouse sIgA Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Secretory Immunoglobulin A (sIgA) Western Blot; Sample: Rat Salivary gland lysate
    Primary Ab: 0.1µg/ml Rabbit Anti-Mouse sIgA Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against sIgA. It has been selected for its ability to recognize sIgA in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Evaluation of the immune benefits of two probiotic strains Bifidobacterium animalis ssp. lactis, BB-12® and Lactobacillus paracasei ssp. paracasei, L. casei 431® in an influenza vaccination model: a randomised, double-blind, placebo-controlled study.Solgar: source
  • RESPON IMUN MUKOSA DAN SELULER PADA TIKUS YANG DIBERI BUBUK SUSU KAMBING DENGAN INFEKSI Salmonella Typhimurium [Mucosal and Cellular Immune Response of Rat Given Goat Milk Powder and Infected with Salmonella Typhimurium]Ipb: Source
  • Effects of Clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88Pubmed: 24570422
  • Potency of Lactobacillus plantarum Dad-13 and Sweet Potato (Ipomoea batatas) Fiber as Immunomodulator in Rats Infected With Salmonella TyphimuriumCcsenet:Source
  • Repeated Oronasal Exposure to Lipopolysaccharide Induced Mucosal IgA Responses in Periparturient Dairy CowsPubmed:25061754
  • Dietary Apostichopus japonicus enhances the respiratory and intestinal mucosal immunity in immunosuppressive micePubmed:25186733
  • Dietary squid ink polysaccharide could enhance SIgA secretion in chemotherapeutic micePubmed:25308407
  • Fecal microbiota transplantation and bacterial consortium transplantation have comparable effects on the re-establishment of mucosal barrier function in mice with intestinal dysbiosisPubMed: 26217323
  • Role of Maternal Allergy on Immune Markers in Colostrum and Secretory Immunoglobulin A in Stools of Breastfed InfantsPubMed: 26243755
  • Oropharyngeal Colostrum Administration in Extremely Premature Infants: An RCTPubMed: 25624376
  • Salivary Secretory Immunoglobulin (SIgA) and Lysozyme in Malignant Tumor Patientsjournals:8701423
  • 益生菌抵御大鼠急性腹泻的效果及其对肠道黏膜屏障的影响article:12422
  • Long-term use of ceftriaxone sodium induced changes in gut microbiota and immune system.pubmed:28220870
  • Imbalance of intestinal immune function in piglets infected by porcine circovirus type 2 during the fetal period.pubmed:28244338
  • Immunomodulatory effects of Hericium erinaceus derived polysaccharides are mediated by intestinal immunologypubmed:28266682
  • Saccharomyces boulardiiadministration can inhibit the formation of gastric lymphoid follicles induced by Helicobacter suis infectionpubmed:28115360
  • Effects of dietary threonine supplementation on intestinal barrier function and gut microbiota of laying henspubmed:28938780
  • Novel dietary polysaccharide SIP promotes intestinal secretory immunoglobulin A secretion in mice under chemotherapy10.1016/j.jff.2017.08.012
  • Evaluation of Susceptibility and Innate Immune Response to Candida albicans in Mice with Sub-healthijp.2018.689.697
  • Correlation of s-IgA and IL-6 Salivary with Caries Disease and Oral Hygiene Parameters in ChildrenPubmed: 31892186
  • Fluorescence polarization-based rapid detection system for salivary biomarkers using modified DNA aptamers containing base-appended basesPubmed: 31855403
  • Artesunate protects immunosuppression mice induced by glucocorticoids via enhancing pro-inflammatory cytokines release and bacterial clearancePubmed: 33045197
  • Effects of different doses lipopolysaccharides on the mucosal barrier in mouse intestinePubmed: 32947071
  • Yacón (Smallanthus sonchifolius) prevented inflammation, oxidative stress, and intestinal alterations in an animal model of colorectal carcinogenesisPubmed: 32567144
  • Construction and Immunogenicity Comparison of Three Virus-Like Particles Carrying Different Combinations of Structural Proteins of Avian Coronavirus Infectious?¡­33670249
  • Ingestion of High ¦Â-Glucan Barley Flour Enhances the Intestinal Immune System of Diet-Induced Obese Mice by Prebiotic Effects33799564
  • Assessment of salivary stress and pain biomarkers and their relation to self-reported pain intensity during orthodontic tooth movement: a longitudinal and prospective …34170330
  • Microarray Analysis of Paramylon, Isolated from Euglena Gracilis EOD-1, and Its Effects on Lipid Metabolism in the Ileum and Liver in Diet-Induced Obese Mice34684408
  • Saliva testing as noninvasive way for monitoring exercise-dependent response in teenage elite water polo players: A cohort study34797320
  • Fermented Corn–Soybean Meal Mixed Feed Modulates Intestinal Morphology, Barrier Functions and Cecal Microbiota in Laying Hens34827791
  • Three Urban of China: A Cohort Study of Maternal-Infant Factors and HM Protein ComponentsPubmed:35438093

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