ELISA Kit for Pulmonary Surfactant Associated Protein A1 (SFTPA1) 
PRL; SFTPA; PSAP; PSPA; SFTP; SPA; COLEC4; PSP-A; SFTP1; SFTPA1B; SP-A1; Collectin-4; Alveolar proteinosis protein; 35 kDa pulmonary surfactant-associated protein; Surfactant Associated Protein A
- UOM
- FOB US$ 441.00 US$ 630.00 US$ 2,835.00 US$ 5,355.00 US$ 44,100.00
- Quantity
Overview
Properties
- Product No.SEA890Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryInfection immunityPulmonology
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Pulmonary Surfactant Associated Protein A1 (SFTPA1) and the recovery rates were calculated by comparing the measured value to the expected amount of Pulmonary Surfactant Associated Protein A1 (SFTPA1) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 96-104 | 101 |
| EDTA plasma(n=5) | 98-105 | 101 |
| heparin plasma(n=5) | 80-90 | 84 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pulmonary Surfactant Associated Protein A1 (SFTPA1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pulmonary Surfactant Associated Protein A1 (SFTPA1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pulmonary Surfactant Associated Protein A1 (SFTPA1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-91% | 93-105% | 92-101% | 78-91% |
| EDTA plasma(n=5) | 96-105% | 92-105% | 95-105% | 80-104% |
| heparin plasma(n=5) | 97-105% | 90-97% | 96-105% | 81-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pulmonary Surfactant Associated Protein A1 (SFTPA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pulmonary Surfactant Associated Protein A1 (SFTPA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pulmonary Surfactant Associated Protein A1 (SFTPA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pulmonary Surfactant Associated Protein A1 (SFTPA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Citations
- Surfactant protein A and D in chronic rhinosinusitis with nasal polyposis and corticosteroid responseIngenta: art00005
- Detection of surfactant proteins A, B, C, and D in human nasal mucosa and their regulation in chronic rhinosinusitis with polypsPubMed: 23406594
- Staphylococcus aureus and Pseudomonas aeruginosa Express and Secrete Human Surfactant ProteinsPubMed: PMC3551896
- The Detection of Surfactant Proteins A, B, C and D in the Human Brain and Their Regulation in Cerebral Infarction, Autoimmune Conditions and Infections of the CNSPubMed: PMC3787032
- Nachweis und Charakterisierung des Oberfl?chenproteins PLUNC (Palate, Lung and Nasal Clone Protein) an der Augenoberfl?che und Bedeutung für das Trockene AugeOpus4:Source
- Expression of surfactant protein-A in exhaled breath condensate of patients with chronic obstructive pulmonary diseasePubMed: 26707652
- Serum Levels of Surfactant Proteins in Patients with Combined Pulmonary Fibrosis and Emphysema (CPFE)Pubmed:27337142
- The Cerebral Surfactant System and Its Alteration in HydrocephalicConditions.pubmed:27656877
- Correlations of Ventricular Enlargement with Rheologically Active SurfactantProteins in Cerebrospinal Fluid.pubmed:28101052
- Correlations of Ventricular Enlargement with Rheologically Active Surfactant Proteins in Cerebrospinal FluidPMC5209370
- Chronic lung injury and impaired pulmonary function in a mouse model of acid ceramidase deficiency.pubmed:29167126
- Metabolic profiling of asthma in mice and the interventional effects of SPA using liquid chromatography and Q-TOF mass spectrometrypubmed:28463380
- Evaluation of surfactant proteins A, B, C, and D in articular cartilage, synovial membrane and synovial fluid of healthy as well as patients with osteoarthritis and …Pubmed: 30235245
- Organic barn dust inhibits surfactant protein D production through protein kinase-c alpha dependent increase of GPR116Pubmed: 30543664
- Elevated levels of multifunctional surfactant proteins in cerebrospinal fluid are associated with signs of increased cerebrospinal fluid flow in cranial magnetic …Doi: 10.1007/s12035-017-0835-5
- Rheologically Essential Surfactant Proteins of the CSF Interacting with Periventricular White Matter Changes in Hydrocephalus Patients–Implications for CSF …Doi: 10.1007/s12035-019-01648-z
- Oxidative Stress and Inflammatory Mediators in Exhaled Breath Condensate of Patients with Pulmonary Tuberculosis. A Pilot Study with a Biomarker Perspective34679707