ELISA Kit for Toll Like Receptor 2 (TLR2) Homo sapiens (Human) Sandwich ELISA

CD282; TIL4; Toll/interleukin-1 receptor-like protein 4

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  • ELISA Kit for Toll Like Receptor 2 (TLR2) Packages (Simulation)
  • ELISA Kit for Toll Like Receptor 2 (TLR2) Packages (Simulation)
  • ELISA Kit for Toll Like Receptor 2 (TLR2) Results demonstration
  • SEA663Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Toll Like Receptor 2 (TLR2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Toll Like Receptor 2 (TLR2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Toll Like Receptor 2 (TLR2)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Toll Like Receptor 2 (TLR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Toll Like Receptor 2 (TLR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Toll Like Receptor 2 (TLR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Toll Like Receptor 2 (TLR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Expression of soluble Toll-like receptors in pleural effusionsCMJ: 2010823483999506297
  • Baicalein Reduces Airway Injury in Allergen and IL-13 Induced Airway InflammationPubMed: PMC3639905
  • TLR2 in Pleural Fluid Is Modulated by Talc Particles during PleurodesisPubMed: PMC3529880
  • The Possible Role of TLR2 in Chronic Hepatitis B Patients with Precore MutationPubmed: 24187552
  • Cervical and vaginal fluid soluble Toll-like receptor 2 in pregnancies complicated by preterm prelabor rupture of membranesPubmed:25028801
  • Cytokines and Toll-Like Receptors in the Immune Response to Influenza VaccinationPubmed:25248345
  • Correlation?between?salivary?Toll like receptor-2?concentration?and early childhood caries.Pubmed:25265998
  • Membrane and soluble Toll‐like receptor 2 in patients with psoriasis treated by Goeckerman therapyPubmed:25266302
  • The discriminative capacity of soluble Toll-like receptor (sTLR)2 and sTLR4 in inflammatory diseasesPubmed:25406630
  • Cathelicidin related antimicrobial peptide, laminin, Toll-like receptors and chemokines levels in experimental hypersensitivity pneumonitis in micePubMed: 25834936
  • Soluble Toll-Like Receptors 2 and 4 in Cerebrospinal Fluid of Patients with Acute Hydrocephalus followingAneurysmal Subarachnoid Haemorrhage.Pubmed:27223696
  • The Effect of Rosuvastatin on Markers of Immune Activation in Treatment-Naive Human Immunodeficiency Virus-PatientsPubmed:26835476
  • Pentraxin 3 and other inflammatory biomarkers related to atrial fibrillation in cardiac surgerypubmed:27852958
  • Soluble TLR2 and 4 concentrations in cerebrospinal fluid in HIV/SIV-relatedneuropathological conditions.pubmed:27882497
  • Toll-like receptors and their soluble forms differ in the knee and thumb basal osteoarthritic jointspubmed:28093922
  • Features of the Pattern Recognition Receptor TLR-2 Expression and Cytokines IL-2, IL-4 Profile in Children with Food Hypersensitivity10.12988:bmgt.2018.812
  • Prospective study on the significance of mucosal barrier dysfunction markers in assessing the effectiveness of the new strategy for treating food hypersensitivity in …10.22141:2224-0551.13.2.2018.129548
  • Vinpocetine regulates levels of circulating TLRs in Parkinson's disease patientsPubmed: 30315378
  • PROGNOSTIC ROLE OF IL-4, IL-2 AND TLR-2 FOR PREDICTING OF COURSE OF FOOD ALLERGY IN CHILDRENDoi: 10.29256/v.02.02.2018.escbm71
  • STUDY OF THE ACTION OF CHROMIUM-CONTAINING BIOPREPARATIONS FROM SPIRULINA ON INSULIN ACTIVITY IN RATSDoi: 10.29256/v.02.02.2018.escbm72
  • Opuntioside, opuntiol and its metallic nanoparticles attenuate adjuvant-induced arthritis: Novel suppressors of Toll-like receptors-2 and-4
  • Aberrant DNA methylation of the toll-like receptors 2 and 6 genes in patients with obstructive sleep apneaPubmed: 32069296
  • ASSOCIATIONS BETWEEN EFFICACY OF THE THERAPY AND CIRCADIAN FLUCTUATIONS OF ENDOTHELIAL NITRIC OXIDE SYNTHASE WITH TOLL-LIKE …Pubmed: 32242853
  • ДОбОвА вАРІАбЕЛЬНІСТЬ РІвНЯ РОзЧИННИХ ТОЛ-пОДІбНИХ РЕцЕпТОРІв 2 в СИРОвАТцІ КРОвІ ЖІНОК, ХвОРИХ НА РЕвМАТОїДНИй АРТРИТ: зв' …
  • FEATURES OF MYOCARDIAL REMODELING IN MEN WITH POST-INFARCTION CARDIOSCLEROSIS AND ITS RELATIONSHIP WITH TOLL-LIKE …
  • Levels of toll-like receptors 2 and 4 in serum of men with postinfarction cardiosclerosis and its relationship with antiphospholipid syndrome components
  • A value of soluble Toll‐like receptor 2 and 4 in vaginally obtained amniotic fluid for the prediction of histological chorioamnionitis34244993
  • Altered innate immune profile in blood of systemic mastocytosis patientsPubmed:35734269

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