Multiplex Assay Kit for Clara Cell Protein 16 (CC16) ,etc. by FLIA (Flow Luminescence Immunoassay) Mus musculus (Mouse) Multiplex ELISA

SCGB1A1; CC10; CCSP; UGB; UG; Uteroglobin; Uteroglobin; Urinary Protein 1; Clara Cell Secretory Protein; Secretoglobin Family 1A Member 1; Clara-Cell Specific 10-kD Protein

(Note: Up to 8-plex in one testing reaction)

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  • Multiplex Assay Kit for Clara Cell Protein 16 (CC16) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Clara Cell Protein 16 (CC16) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Clara Cell Protein 16 (CC16) ,etc. by FLIA (Flow Luminescence Immunoassay) Results demonstration
  • LMA857Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Clara Cell Protein 16 (CC16) and the recovery rates were calculated by comparing the measured value to the expected amount of Clara Cell Protein 16 (CC16) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-104 89
EDTA plasma(n=5) 97-105 101
heparin plasma(n=5) 86-101 89
sodium citrate plasma(n=5) 82-96 90

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Clara Cell Protein 16 (CC16) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Clara Cell Protein 16 (CC16) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Clara Cell Protein 16 (CC16) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-101% 83-97% 81-94% 82-92%
EDTA plasma(n=5) 85-101% 96-104% 97-105% 88-97%
heparin plasma(n=5) 78-96% 88-105% 78-101% 94-103%
sodium citrate plasma(n=5) 86-98% 91-99% 80-91% 78-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:CC16) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Clara Cell Protein 16 (CC16) ,etc. by FLIA (Flow Luminescence Immunoassay)

Test principle

Anti-Clara Cell Protein 16 (CC16) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Clara Cell Protein 16 (CC16) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Clara Cell Protein 16 (CC16) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Clara Cell Protein 16 (CC16) in the sample and corresponding value of MFI (Median Fluorescence Intensity).

Giveaways

Citations

  • Pulmonary functions and blood biochemical markers for workers with and without coal worker pneumoconiosisSciencepub: Source
  • Analysis of the Protective Biochemical and Pathologic Effects of Aminoguanidine on an Experimental Aspiration Pneumonitis Model Induced by Bile AcidsPubMed: S0011393X12000562
  • Effects of leflunomide on inflamation and fibrosis in bleomycine induced pulmonary fibrosis in wistar albino ratsPubmed: 24255778
  • The therapeutic effects of anti-oxidant and anti-inflammatory drug quercetin on aspiration-induced lung injury in ratsPubmed: 24122261
  • The effects of α-tocopherol on oxidative damage and serum levels of Clara cell protein 16 in aspiration pneumonitis induced by bile acidsPubmed: 23023024
  • The evaluation of different treatment protocols for trauma-induced lung injury in ratsPubmed: 24605218
  • Susceptibility to COPD: Differential Proteomic Profiling after Acute SmokingPubmed:25036363
  • Urine proteome analysis by C18 plate–matrix-assisted laser desorption/ionization time-of-flight mass spectrometry allows noninvasive differential diagnosis …Pubmed:30024955
  • N-Acetylcysteine inhalation improves pulmonary function in patients received Liver Transplantation.Doi: 10.1042/BSR20180858
  • Mechanical activation of TRPV4 channels controls albumin reabsorption by proximal tubule cells.
  • An ultrasensitive electrochemical detection of tryptase using 3D macroporous reduced graphene oxide nanocomposites by one-pot electrochemical synthesis
  • Effects of mammary nutrient demand on glucose kinetics of dairy cows in early lactation
  • Role of bone marrow-derived mesenchymal stem cells in alleviating pulmonary epithelium damage and extracellular matrix remodeling in a rat model of lung fibrosis …Pubmed: 32909452
  • The Protective Effects of Butorphanol on Pulmonary Function of Patients with Obesity Undergoing Laparoscopic Bariatric Surgery: a Double-Blind Randomized …Pubmed: 32535786
  • Elevated plasma levels of epithelial and endothelial cell markers in COVID-19 survivors with reduced lung diffusing capacity six months after hospital …Pubmed:35189887
  • Lung-Protective Effects of Lidocaine Infusion on Patients with Intermediate/High Risk of Postoperative Pulmonary Complications: A Double-Blind Randomized …Pubmed:35422611

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