Multiplex Assay Kit for Cyclooxygenase-2 (COX 2) ,etc. by CBA (Cytometric Bead Array) Mus musculus (Mouse) Protein, Antibody, Assaykit, Service

PGG/HS; PGHS2; PHS2; HCox2; PTGS2; Prostaglandin Endoperoxide Synthase 2; Prostaglandin G/H Synthase And Cyclooxygenase; Prostaglandin H2 synthase 2

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  • Multiplex Assay Kit for Cyclooxygenase-2 (COX 2) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Cyclooxygenase-2 (COX 2) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Cyclooxygenase-2 (COX 2) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Cyclooxygenase-2 (COX 2) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-101 89
EDTA plasma(n=5) 89-103 92
heparin plasma(n=5) 88-96 91

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyclooxygenase-2 (COX 2) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyclooxygenase-2 (COX 2) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cyclooxygenase-2 (COX 2) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 92-103% 78-103% 84-105% 79-92%
EDTA plasma(n=5) 93-102% 80-104% 91-99% 83-102%
heparin plasma(n=5) 94-102% 95-102% 78-103% 90-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Cyclooxygenase-2 (COX 2) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Cyclooxygenase-2 (COX 2) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Cyclooxygenase-2 (COX 2) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Cyclooxygenase-2 (COX 2) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Cyclooxygenase-2 (COX 2) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.

Giveaways

Citations

  • Diallyl sulfide protects against ultraviolet B-induced skin cancers in SKH-1 hairless mouse: analysis of early molecular events in carcinogenesisWiley: source
  • Molecular mechanisms underlying chemopreventive activities of glycyrrhizic acid against UVB-radiation-induced carcinogenesis in SKH-1 hairless mouse epidermisPubMed: 21545294
  • Curcumin Protects against UVB-Induced Skin Cancers in SKH-1 Hairless Mouse: Analysis of Early Molecular Markers in CarcinogenesisPubMed: 22888366
  • Decalepis hamiltonii inhibits tumor progression and metastasis by regulating the inflammatory mediators and nuclear factor κB subunitsPubmed: 24013642
  • Multitargeted protective effect of Abacopteris penangiana against carrageenan-induced chronic prostatitis in rats.Pubmed: 24211397
  • Relationship between epidermal growth factor receptor (EGFR) mutation and serum cyclooxygenase-2 Level, and the synergistic effect of celecoxib and gefitinib on EGFR expression in non-small cell lung cancer cellsPubMed: 26464643
  • Monitoring of cyclooxygenase-2 levels can predict EGFR mutations and the efficacy of EGFR-TKI in patients with lung adenocarcinomaPubMed: 26191267
  • The shear wave elastic modulus and the increased nuclear factor kappa B(NF-kB/p65) and cyclooxygenase-2 (COX-2) expression in the area of myofascial trigger points activated in a rat model by blunt trauma to the vastus medialispubmed:29137729
  • Inflammatory Response of Annona Muricata Linn Leaves Extract in Colorectal Cancer PatientsNCT02439580
  • High Expression of IL-1RI and EP2 Receptors in the IL-1β/COX-2 Pathway, and a New Alternative to Non-Steroidal Drugs—Osthole in Inhibition COX-2Pubmed: 30620999
  • A novel concept of immunological and allergy interactions in Autism Spectrum Disorders: Molecular, anti-inflammatory effect of ostholePubmed: 30953868
  • Evaluation of Serum Cyclooxygenase, Hepcidin Levels in Acute Renal Injury (AKI) Patients Following Cardiac Catheterization
  • Isolation and characterization of anti-inflammatory and anti-proliferative compound, for B-cell Non-Hodgkin lymphoma, from Nyctanthes arbor-tristis Linn.Pubmed:35398498

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