OneStep ELISA Kit for Clara Cell Protein 16 (CC16) Homo sapiens (Human) Protein, Antibody, Assaykit, Service

SCGB1A1; CC10; CCSP; UGB; UG; Uteroglobin; Uteroglobin; Urinary Protein 1; Clara Cell Secretory Protein; Secretoglobin Family 1A Member 1; Clara-Cell Specific 10-kD Protein

  • UOM
    1. 48T
    2. 96T
  • FOB US$ 485.00 US$ 693.00
  • Quantity
Add to Cart Distributors
Overview
Properties
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • OneStep ELISA Kit for Clara Cell Protein 16 (CC16) Packages (Simulation)
  • OneStep ELISA Kit for Clara Cell Protein 16 (CC16) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Clara Cell Protein 16 (CC16) and the recovery rates were calculated by comparing the measured value to the expected amount of Clara Cell Protein 16 (CC16) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 85-94 91
EDTA plasma(n=5) 93-103 101
heparin plasma(n=5) 85-92 88

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Clara Cell Protein 16 (CC16) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Clara Cell Protein 16 (CC16) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Clara Cell Protein 16 (CC16) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-101% 99-105% 84-98% 89-96%
EDTA plasma(n=5) 80-94% 80-90% 83-93% 88-103%
heparin plasma(n=5) 89-96% 91-99% 90-99% 90-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well, then add 50µL prepared Detection Reagent A to each well. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
5. Add 50µL Stop Solution. Read at 450nm immediately.

OneStep ELISA Kit for Clara Cell Protein 16 (CC16)

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to CC16. Standards or samples are then added to the appropriate microplate wells with a HRP-conjugated antibody specific to CC16. After TMB substrate solution is added, only those wells that contain CC16 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CC16 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Pulmonary functions and blood biochemical markers for workers with and without coal worker pneumoconiosisSciencepub: Source
  • Analysis of the Protective Biochemical and Pathologic Effects of Aminoguanidine on an Experimental Aspiration Pneumonitis Model Induced by Bile AcidsPubMed: S0011393X12000562
  • Effects of leflunomide on inflamation and fibrosis in bleomycine induced pulmonary fibrosis in wistar albino ratsPubmed: 24255778
  • The therapeutic effects of anti-oxidant and anti-inflammatory drug quercetin on aspiration-induced lung injury in ratsPubmed: 24122261
  • The effects of α-tocopherol on oxidative damage and serum levels of Clara cell protein 16 in aspiration pneumonitis induced by bile acidsPubmed: 23023024
  • The evaluation of different treatment protocols for trauma-induced lung injury in ratsPubmed: 24605218
  • Susceptibility to COPD: Differential Proteomic Profiling after Acute SmokingPubmed:25036363
  • Urine proteome analysis by C18 plate–matrix-assisted laser desorption/ionization time-of-flight mass spectrometry allows noninvasive differential diagnosis …Pubmed:30024955
  • N-Acetylcysteine inhalation improves pulmonary function in patients received Liver Transplantation.Doi: 10.1042/BSR20180858
  • Mechanical activation of TRPV4 channels controls albumin reabsorption by proximal tubule cells.
  • An ultrasensitive electrochemical detection of tryptase using 3D macroporous reduced graphene oxide nanocomposites by one-pot electrochemical synthesis
  • Effects of mammary nutrient demand on glucose kinetics of dairy cows in early lactation
  • Role of bone marrow-derived mesenchymal stem cells in alleviating pulmonary epithelium damage and extracellular matrix remodeling in a rat model of lung fibrosis …Pubmed: 32909452
  • The Protective Effects of Butorphanol on Pulmonary Function of Patients with Obesity Undergoing Laparoscopic Bariatric Surgery: a Double-Blind Randomized …Pubmed: 32535786
  • Elevated plasma levels of epithelial and endothelial cell markers in COVID-19 survivors with reduced lung diffusing capacity six months after hospital …Pubmed:35189887
  • Lung-Protective Effects of Lidocaine Infusion on Patients with Intermediate/High Risk of Postoperative Pulmonary Complications: A Double-Blind Randomized …Pubmed:35422611

Recommend products