OneStep ELISA Kit for Peptide YY (PYY) Mus musculus (Mouse) Protein, Antibody, Assaykit, Service

PYY1; Peptide tyrosine tyrosine

  • UOM
    1. 48T
    2. 96T
  • FOB US$ US$
  • Quantity
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  • OneStep ELISA Kit for Peptide YY (PYY) Packages (Simulation)
  • OneStep ELISA Kit for Peptide YY (PYY) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Peptide YY (PYY) and the recovery rates were calculated by comparing the measured value to the expected amount of Peptide YY (PYY) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 93-101 96
EDTA plasma(n=5) 89-103 94
heparin plasma(n=5) 92-101 96

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peptide YY (PYY) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peptide YY (PYY) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Peptide YY (PYY) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 95-103% 99-105% 79-99% 83-92%
EDTA plasma(n=5) 96-105% 91-98% 94-102% 80-98%
heparin plasma(n=5) 90-98% 86-97% 87-98% 78-90%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well, then add 50µL prepared Detection Reagent A to each well. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
5. Add 50µL Stop Solution. Read at 450nm immediately.

OneStep ELISA Kit for Peptide YY (PYY)

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to Peptide YY (PYY) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between HRP labeled Peptide YY (PYY) and unlabeled Peptide YY (PYY) (Standards or samples) with the pre-coated antibody specific to Peptide YY (PYY). After incubation, the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of Peptide YY (PYY) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Peptide YY (PYY) in the sample.

Citations

  • The Effects of Concurrent Resistance and Endurance Exercise on Hunger Feelings and PYY in Obese MenCom:Source
  • Effect of an Acute Incremental Exercise on Plasma Peptide YY, Neuropeptide Y and IGF-1 Concentrations in Young AthletesAassjournal:Source
  • Calprotectin in serum and zonulin in serum and feces are elevated after introduction of a diet with lower carbohydrate content and higher fiber, fat and protein contents.pubmed:28413639
  • Effect of lifestyle improvement program on the biomarkersof adiposity, inflammation and gut hormones in overweight/obese Asian Indians with prediabetespubmed:28620678
  • Calprotectin in serum and zonulin in serum and feces are elevated after introduction of a diet with lower carbohydrate content and higher fiber, fat and protein contents10.3892/br.2017.865
  • High Fiber Fat and Protein Contents Lead to Increased Satiety Reduced Sweet Cravings and Decreased Gastrointestinal Symptoms Independently of Anthropometric Hormonal and Metabolic Factors10.4172/2155-6156.1000733 
  • Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21Pubmed:29593555
  • Alignments of endocrine, anthropometric, and metabolic parameters in type 2 diabetes after intervention with an Okinawa-based Nordic dietPubmed:29599686
  • Grape Seed Proanthocyanidin Affects Lipid Metabolism via Changing Gut Microflora and Enhancing Propionate Production in Weaned PigsPubmed: 31175811
  • Bile acid receptor TGR5 is critically involved in preference for dietary lipids and obesityPubmed: 31812910
  • Serotonin involvement in okadaic acid-induced diarrhoea in vivo34148100
  • Appetite and Satiety Effects of the Acute and Regular Consumption of Green Coffee Phenols and Green Coffee Phenol/Oat β-Glucan Nutraceuticals in Subjects with …34828792

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