-
, A brief introduction of “Fibulin-3 as a Blood and Effusion Biomarker for Pleural Mesothelioma”
Pleural mesothelioma is the tumor that originated in the peritoneal mesothelial cell. Pleural mesothelioma is related with asbestos exposure. There are significant differences in the incidence of pleural mesothelioma in different countries, this is mainly related to the asbestos consumption of these countries over the past several decades. The mortality rate of pleural mesothelioma has a rising tendency in recent years.The common first symptom of pleural mesothelioma is chest pain, cough and shortness of breath. There are also symptoms of fever, sweating or joint pain. Most patients have a lot of pleural effusion and short of breath, the patients that without mass of pleural effusion w......
-
, RNAi technology — a new perspective on life
Since September 2004, "Nature" published the first article on the results of RNA interference by American scientist Andrew Z. Fire and Craig C. Mello et al., The small RNA playsthe important regulatory role in biological life activities. Every day there are articles on relation of the various small RNA and body development. And smart biotechnology companies are also at an amazing rate to actively use RNAi technology fordrug screening,Cloud-Clone Corp. has been launched for the lentivirus and adenovirus expression system, The RNAi is one of the important sever of customer.RNAi is a specific gene silencing technology,which is mediated by double-stranded RNA (dsRNA). RNAi bl......
-
, Real Time qPCR, a gene real-time monitoring expert
Gene is regarded as the most important information carrier of organism. It stores all the genetic information of organisms, determines the biological characteristics, classification, life cycle and other characteristics. However, genes don’t directly join in the regulation of life activities. In higher organisms, DNA is served as template to get RNA by transcription, some RNA guide making protein by translation, the protein is directly joined in kinds of life activities. RNA play an important role connecting gene and protein. The RNA will directly or indirectly take part in the process of protein translation, gene regulation and nucleic acid cutting. All of these support that the study......
-
, Conventional cell proliferation detection techniques
Cells have to split the proliferation of the way. Single-celled organisms, which produce new members by cell division. Multicellular organisms, which produce new cells in a split way to supplement the aging or death cells in the body. Mitosis is the main mode for cell divisionof eukaryotes. Cell proliferation is the basis of organism growth, development, breeding, genetics, and so on, so cell proliferation detection has always been a hot research topic.Conventional cell proliferation detection techniques include the following:(1)Marker protein detection by WB and so on. PCNAis proliferating cell nuclear antigen, it plays an important role in starting DNA replication and cell prolifera......
-
, C Reactive Protein, is it just a Bio-maker of Inflammation?
It is known that the blood routing testsand one of them is C reactive protein(CRP) test. But who knows what CRP and its clinical significanceis?CRP is a protein which is produced by liver, ithas 224 amino acids and themonomer molecular mass of 25kDa. CRP can bind C polysaccharide on the surface of bacteria to form a complex to activate the classical complement pathway, which allows body to obtain the ability by nonspecific immune pathway to resist various bacteria, fungi and parasites, etc. In normal individuals, the CRP level in blood ranges from 800to 80000ug/L. And for organism with acute infection, serious injury or malignant tumor, the CRP level will rise dramatically in the next ......
-
, Prothrombin Fragment 1+2 (F1+2) - Molecular Marker for Early Diagnosis of Thrombotic Diseases
Prothrombin Fragment 1+2(F1+2) is stable degradation product when the prothrombin is activated to thrombin. The F1+2 level in blood can reflect the activation level of thrombin in early stage, which can be used to diagnose the prethrombotic state & thrombotic diseases, remedy these diseases and monitor the efficacy.Take human F1+2 for example, the intrinsic and extrinsic pathways activate factor X to Xa, which removes the activation peptide and cleaves the remaining part of prothrombin into light and heavy chains by cutting the peptide bonds between Arg327-Thr328 and Arg363-Ile364 at same time, and then the F1+2(Ala44~Arg327) is released. While the thrombin can act on the prothrombin t......