DAB Chromogen Kit

Instruction manual

FOR IN VITRO AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

First Edition (Revised on April, 2016)

INTENDED USE

DAB Chromogen Kit is a widely used chromogen for immunohistochemical staining and immunoblotting.

PRINCIPLE

DAB (3,3N-Diaminobenzidine Tertrahydrochloride) is one of the most important chromogens for peroxidase. In the presence of peroxidase enzyme, a dark brown precipitate will result.

REAGENTS

Reagents	Amounts	Ingredients
Chromogen A (1×)	5mL*2	Peroxide
Chromogen B (50×)	200μL	DAB

STORAGE OF THE KIT

It is recommended to store chromogen A and chromogen B at 4oC. The expiration date of the kit is about 6 months.

ASSAY PROCEDURES

1. Preparation: Mix 1mL chromogen A with 20μL chromogen B thoroughly, and then add this solution to the sample. Observe under a microscope to verify the ending time. Generally, 10 sec to 15 min is enough.
2. Fully soak sample with distilled water to end the reaction.
3. Chromogen B becoming darker has no effect when use.
4. DAB is a suspected carcinogen. Please take appropriate care when using it.