Active Activin A (ACVA) Homo sapiens (Human) Active protein

ACV-A; Activin Beta A Beta A Homodimer

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Overview
Properties
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point9.3
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  • Active Activin A (ACVA) Packages (Simulation)
  • Active Activin A (ACVA) Packages (Simulation)
  • APA001Hu01.jpg Figure. SDS-PAGE
  • Active Activin A (ACVA) WB Image
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Activin A (ACVA) is a member of the TGF-β superfamily of cytokines and involved in a wide range of biological processes including tissue morphogenesis and repair, fibrosis, inflammation, neural development, hematopoiesis, reproductive system function, and carcinogenesis. To test the effect of ACVA on cell apoptosis, A549 cells were seeded into 96-well plates at a density of 3,000 cells/well with 1% serum standard DMEM including various concentrations of recombinant human ACVA. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 2 hours at 37℃. Proliferation of A549 cells after incubation with ACVA for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant human ACVA for 48h. The result was shown in Figure 2. It was obvious that ACVA significantly inhibit cell viability of A549 cells. The ED50 is 5.09 μg/mL.

Figure 2. Inhibition of A549 cells proliferation after stimulated with ACVA.

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Secretory Products From Epicardial Adipose Tissue of Patients With Type 2 Diabetes Mellitus Induce Cardiomyocyte DysfunctionAhajournals: Source
  • Article: Hksobf_2015_V46N2_167
  • Methods for Diagnosis and Treatment of Concussion or Brain Injury2016:0103140
  • Activins and their related proteins in colon carcinogenesis: insights from early and advanced azoxymethane rat models of colon cancer.pubmed:27835986
  • Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4+ T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling.pubmed:28032440
  • Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4 + T Helper 17(Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signallingdoi:10.1111
  • MicroRNA-27b Modulates Inflammatory Response and Apoptosis during Mycobacterium tuberculosis InfectionPubmed:29661829
  • Expression of platelet-derived growth factor B is upregulated in patients with thoracic aortic dissectionPubmed:29685513
  • Toxic effects of arsenic trioxide on protoscoleces through ROS production, and Ca-ER stress-dependent apoptosis10.1093:abbs
  • The effect of raw and fermented rapeseed cake on the metabolic parameters, immune status, and intestinal morphology of turkeysPubmed:29917099
  • Characterization of Phospholipids from Pacific Saury () Viscera and Their Neuroprotective Activity10.1016:j.fbio.2018.06.002
  • Single Wall Carbon Nanotube and Magnetic Bead Based Electrochemical Immunosensor for Sensitive Detection of Salivary Secretory Immunoglobulin A10.2174:2211556006666170525161225
  • FRIDAY, NOVEMBER 3, 2017 11.30-12.0 BALLROOM AB:
  • SEROPREVALENCE OF ANTI-MANNOSE BINDING LECTIN AUTOANTIBODIES IN PATIENTS WITH RHEUMATOID ARTHRITIS IN SANA'A CITY-YEMEN10.22270:ujpr.v3i2.138
  • Involvement of RBP4 in diabetic atherosclerosis and the role of vitamin D intervention:
  • Gingival crevicular fluid levels of human beta-defensin-1 in type 2 diabetes mellitus and periodontitisPubmed:29713888
  • Myocardial fibrosis in hypertrophic cardiomyopathy:
  • TELLING TRANSVERSAL COMPETENCES… TO BE PROFESSIONALLY PROMOTED:
  • Protein biomarker panels for detecting colorectal cancer and advanced adenoma:
  • Endothelotropic activity of 4-hydroxy-3, 5-di-tret-butylcinnamic acid in the conditions of experimental cerebral ischemia:
  • Silencing of the CSNK2β gene by siRNA inhibits invasiveness and growth of MDA-MB-231 cells10.1101:311092
  • Flow Cytometry-Defined CD49d Expression in Circulating T-Lymphocytes Is a Biomarker for Disease Progression in Duchenne Muscular DystrophyPubmed:29067667
  • SS
  • PP
  • DD
  • hh
  • OO
  • MM
  • EE
  • II
  • BB
  • TT
  • NN
  • LL
  • JJ
  • Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumorsPubmed: 30952517
  • The performances of serum activins and follistatin in the diagnosis of ectopic pregnancy: A prospective case-control studyPubmed: 31647905
  • The Association between the Activin A Serum Level and Carotid Intima-Media Thickness in Chronic Kidney Disease Patients
  • Profiling Activins and Follistatin in Colorectal Cancer According to Clinical Stage, Tumour Sidedness and Smad4 Status34867090

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