Active Caspase 1 (CASP1) Rattus norvegicus (Rat) Active protein

ICE; IL1BC; IL1-BC; P45; Interleukin 1 Beta Converting Enzyme; Apoptosis-Related Cysteine Peptidase; Interleukin-1 beta convertase

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Overview
Properties
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point7.7
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  • Active Caspase 1 (CASP1) Packages (Simulation)
  • Active Caspase 1 (CASP1) Packages (Simulation)
  • APB592Ra01.jpg SDS-PAGE
  • Active Caspase 1 (CASP1) Figure. Western Blot; Sample: Recombinant CASP1, Rat.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Caspase 1 (CASP1) cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. It also has lots of other function, such as defenses against pathogens, cleaves and activates sterol regulatory element binding proteins (SREBPs), promotes apoptosis. Besides, Interleukin 1 Alpha (IL1a) has been identified as an interactor of CASP1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CASP1 and recombinant rat IL1a. Briefly, CASP1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1a-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CASP1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of CASP1 and IL1a was shown in Figure 1, and this effect was in a dose dependent manner.

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-ReperfusionPubmed:24816434
  • Inflammosome in the human endometrium: further step in the evaluation of the “maternal side”PubMed: 26474737
  • Synthetic PreImplantation Factor (PIF) prevents fetal loss by modulating LPS induced inflammatory response.pubmed:28704412
  • NS1 protein of 2009 pandemic influenza A virus inhibits porcine NLRP3 inflammasome-mediated interleukin-1 beta production by suppressing ASC ubiquitinationPubmed:29386291
  • Recurrent pregnancy loss is associated to leaky gut: a novel pathogenic model of endometrium inflammation?Pubmed:29665864
  • A role for the inflammasome in spontaneous labor at termPubmed:27852921
  • CHARACTERIZATION OF PORCINE NLRP3 INFLAMMASOME-MEDIATED INTERLEUKIN-1 BETA PRODUCTION IN RESPONSE TO INFLUENZA A VIRUSESPubmed: 29386291
  • NLRP3 inflammasome expression in lipopolysaccharide-induced otitis mediaPubmed: 30676848
  • Effect of alpha lipoic acid and myoinositol on endometrial inflammasome from recurrent pregnancy loss womenPubmed: 31148259
  • Serum Caspase-1 levels in women with polycystic ovary syndromePubmed: 32127139
  • Increased Level of Caspase-1 in the Serum of Relapsing-remitting Multiple Sclerosis (RRMS) Patients
  • ACTIVITY OF APOPTOSIS MARKERS IN HPV-ASSOCIATED CERVICAL PATHOLOGIES
  • CLINICAL AND PATHOGENETIC SIGNIFICANCE OF CHANGES IN PURINE METABOLISM DURING LUNG CANCER
  • The NLRP3 inhibitor dapansutrile attenuates folic acid induced nephrotoxicity via inhibiting inflammasome/caspase-1/IL axis and regulating autophagy/proliferation34560082
  • The alarmin S100A12 causes sterile inflammation of the human chorioamniotic membranes and preterm birth and neonatal mortality in mice34632484
  • TRPV1 Suppressed NLRP3 Through Regulating Autophagy in Microglia After Ischemia-Reperfusion InjuryPubmed:35041191
  • Influenza A Virus Infection Activates NLRP3 Inflammasome through Trans-Golgi Network DispersionPubmed:35062292

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