Active Interleukin 12 (IL12) Homo sapiens (Human) Active protein

p70

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point5.7
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  • Active Interleukin 12 (IL12) Packages (Simulation)
  • Active Interleukin 12 (IL12) Packages (Simulation)
  • APA111Hu61.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Interleukin 12 (IL12) is a heterodimeric cytokine composed of p35 (IL12A) and p40 (IL12B) subunits, primarily produced by dendritic cells, macrophages, and B cells. It plays a critical role in bridging innate and adaptive immunity by promoting Th1 differentiation, enhancing IFN-γ production, and stimulating NK and T cell cytotoxicity. IL12 is pivotal in anti-tumor and anti-infectious responses but dysregulation can contribute to autoimmunity.IL12 binds IL12Rb1 (a subunit of IL12 receptor) with high affinity, initiating JAK-STAT signaling.Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human IL12 and recombinant mouse IL12Rb1 .Briefly, biotin-linked IL12 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to IL12Rb1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of IL12 and IL12Rb1 was shown in Figure 1, the EC50 for this effect is 0.37ug/mL.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Characterization of Simian Immunodeficiency Virus Variants Anatomically Compartmentalized inPlasma and Milk in Chronically Infected African Green Monkeys.pubmed:
  • A Novel Potential Strategy for Enhancing Antigen Presentation of Human Hepatocellular Carcinoma Cells Propagated ex-vivo Using a Ribonuclease Enzyme …Scholarena:Source
  • Switched memory B cells promote alveolar bone damage during periodontitis: An adoptive transfer experimentPubmed:30015235
  • Antitumor efficacy of the Runx2‑dendritic cell vaccine in triple‑negative breast cancer in vitroPubmed:30127867
  • The P2X7 receptor, cathepsin S and fractalkine in the trigeminal subnucleus caudalis signal persistent hypernociception in temporomandibular rat jointsPubmed: 30248434
  • Soluble LAG3 acts as a potential prognostic marker of gastric cancer and its positive correlation with CD8+ T cell frequency and secretion of IL-12 and INF-γ in …Pubmed: 30223387
  • The relationship between autophagy and apoptosis of lamina propria dendritic cells from the small intestine on the immunodynamics of sepsis mice
  • ВЛИЯНИЕ ДНК МЕТИЛТРАНСФЕРАЗЫ 3А И ГИСТОНДЕАЦЕТИЛАЗЫ НА СОДЕРЖАНИЕ В МОНОНУКЛЕАРНЫХ КЛЕТКАХ ЦЕЛЬНОЙ КРОВИ …
  • RBP and Alb in neonatal hydronephrosis and its association with IL-12 levels in pregnant women before delivery
  • Atmospheric H2S triggers immune damage by activating the TLR-7/MyD88/NF-κB pathway and NLRP3 inflammasome in broiler thymus
  • Disseminated And Infection Accompanied Sweet's Syndrome In A Patient With Anti-Interferon-γ Autoantibodies: A Case Report.Pubmed: 31632104
  • Suppression of Fpr2 expression protects against endotoxin-induced acute lung injury by interacting with Nrf2-regulated TAK1 activationPubmed: 32106380
  • Silkworm pupa oil attenuates acetaminophen‐induced acute liver injury by inhibiting oxidative stress‐mediated NF‐κB signalingPubmed: 31993149
  • Innate immune response of mammary gland induced by intramammary infusion of Bifidobacterium breve in lactating dairy cowsPubmed: 33071254
  • Vasküler Demans Modelinde Çevresel Zenginleştirmenin Depresyon Benzeri Davranış, Kortikal ve Hipokampal BDNF ve IL-1β Seviyeleri Üzerine Etkisi
  • Evaluation of lipopolysaccharide-induced acute lung injury attenuation in mice by Glycyrrhiza glabra
  • BBG enhances OLT1177-induced NLRP3 inflammasome inactivation by targeting P2X7R/NLRP3 and MyD88/NF-¦ÊB signaling in DSS-induced colitis in rats33548287
  • HOXA5 induces M2 macrophage polarization to attenuate carotid atherosclerosis by activating MED134117711
  • Establishing novel roles of bifidocin LHA, antibacterial, antibiofilm and immunomodulator against Pseudomonas aeruginosa corneal infection model34171261
  • Combined treatment with epigenetic agents enhances anti-tumor activity of T cells by upregulating the ACRBP expression in hepatocellular carcinoma34377237
  • Cytokine profile in serum and gingival crevicular fluid of children with inflammatory bowel disease: A case‐control study34730850
  • Exploring Epithelial–Mesenchymal Transition Signals in Endometriosis Diagnosis and In Vitro Fertilization Outcomes34829910
  • Biomimetic GBM-targeted drug delivery system boosting ferroptosis for immunotherapy of orthotopic drug-resistant GBMPubmed:35351131
  • Cyclosporine A loaded brain targeting nanoparticle to treat cerebral ischemia/reperfusion injury in micePubmed:35658867
  • Deletion of Toxoplasma Rhoptry Protein 38 (PruΔrop38) as a Vaccine Candidate for Toxoplasmosis in a Murine ModelPubmed:35740356

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