Active Nesfatin 1 (NES1) Mus musculus (Mouse) Active protein

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Overview
Properties
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 97%
  • Isoelectric Point5.8
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  • Active Nesfatin 1 (NES1) Packages (Simulation)
  • Active Nesfatin 1 (NES1) Packages (Simulation)
  • APA242Mu01.jpg SDS-PAGE
  • Active Nesfatin 1 (NES1) Figure. Western Blot; Sample: Recombinant NES1, Mouse.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Nucb2 (Nucleobindin-2), a calcium-binding protein, is further cleaved into NES1 (Nesfatin-1). NES1 is an anorexigenic peptide and seems to participate in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. GADD45A (Growth arrest and DNA damage-inducible protein GADD45 alpha) has been identified as an interactor of Nucb2. Besides, rat GADD45A shares similarities with mouse NES1 in amino acid sequence with the identity of 98%. Thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse NES1 and recombinant rat GADD45A. Briefly, NES1 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL NES1 were then transferred to GADD45A-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-NES1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of NES1 and GADD45A was shown in Figure 1, and this effect was in a dose dependent manner.

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Plasma Nesfatin-1 Level May Be Associated with Disease Severity in Patients with Panic DisorderPsikofarmakoloji: 2043
  • High plasma nesfatin-1 level in patients with major depressive disorderPubMed: 21163317
  • Cord blood nesfatin-1 and apelin-36 levels in gestational diabetes mellitusSpringerLink: h978088657555w16
  • Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsive Disorder Psikofarmakoloji: 2212
  • Decreased plasma nesfatin-1 levels in patients with generalized anxiety disorderPubMed: 22595767
  • Effects of Aerobic Training, with or without Zizyphus Jujuba Water Extraction, on Fundus Nesfatin-1, ATP, HDL-C, and LDL-C Concentrations in Female RatsIjvst: Source
  • Decreased serum nesfatin-1 levels in endometriosisPubmed: 24702902
  • Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsie Disorder.Psikofarmakoloji: Source
  • Role of NUCB2/nesfatin-1 as a possible biomarker.Pubmed: 23537082
  • A role of nesfatin-1/NucB2 in dehydration-induced anorexiaPubmed:24829503
  • Plasma nesfatin-1 levels are increased in patients with polycystic ovary syndromePubmed:24920281
  • The potential role of BMI, plasma leptin, nesfatin-1 and ghrelin levels in the early detection of pancreatic necrosis and severe acute pancreatitis: a prospective cohort study.Pubmed:25448651
  • The effects of short-term high-intensity interval training vs. moderate-intensity continuous training on plasma levels of nesfatin-1 and inflammatory markersPubmed:25581765
  • New hormones to predict the severity of gallstone-induced acute pancreatitisPubmed:25599787
  • Is There a Relationship between Obstructive Sleep Apnea Syndrome Severity and Nesfatin-1?PubMed: 26088934
  • Correlation of Brain Neuropeptide (Nesfatin-1 and Orexin-A) Concentrations with Anthropometric and Biochemical Parameters in Malnourished ChildrenPubMed: 26831553
  • Nesfatin-1 and Vitamin D levels may be associated with systolic and diastolic blood pressure values and hearth rate in polycystic ovary syndromePubMed: 26295295
  • Relationship and Interaction between Anaerobic Sports Branches and Serum Nesfatin-1Sayilar: 294
  • Nesfatin-1 levels and metabolic markers in polycystic ovary syndromePubMed: 26062107
  • Serum nesfatin-1 levels: a potential new biomarker in patients with subarachnoid hemorrhage*Pubmed:26872406
  • Comparing the nesfatin-1 levels between individuals with subjective cognitive decline and mild cognitive impairmentP.1.e.012.pdf
  • Serum nesfatin-1 levels: a potential new biomarker in patients with subarachnoid hemorrhage.pubmed:26872406
  • Metabolic and Cardiovascular Actions of Nesfatin-1: Implications in Health and Diseasepubmed:28137218
  • Serum nesfatin-1 levels are decreased in pregnant women newly diagnosed with gestational diabetespubmed:28977160
  • Serum NUCB2/nesfatin-1 levels in different stages of alcohol dependence: Is there a relationship with craving?pubmed:28529367
  • Nesfatin-1 promotes VSMC migration and neointimal hyperplasia by upregulating matrix metalloproteinases and downregulating PPARγPubmed:29604590
  • Nesfatin-1 functions as a switch for phenotype transformation and proliferation of VSMCs in hypertensive vascular remodelingPubmed:29627363
  • Plasma nesfatin-1 level is associated with severity of depression in Chinese depressive patientsPubmed:29615007
  • Mitra Zarrati, Mahsa Raji Lahiji, Eisa Salehi, Bahareh Yazdani, Elham Razmpoosh, Raheleh Shokouhi Shoormasti & Farzad ShidfarPubmed: 30232744
  • An elevated serum level of nesfatin-1 may predict poor outcomes in patients with aneurysmal subarachnoid hemorrhages
  • Relationshıp of spontaneous subarachnoid haemorrhage and cerebral aneurysm to serum Visfatin and Nesfatin-1 levelsPubmed: 32311618
  • Copeptin and Nesfatin-1 Are Interrelated Biomarkers with Roles in the Pathogenesis of Insulin Resistance in Chinese Children with ObesityPubmed: 33027789
  • Assessing the blood concentration of new adipocytokines in patients with ischaemic stroke33283259
  • Increased serum nesfatin-1 levels among adolescents diagnosed with major depressive disorder

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