Recombinant Alanine Aminotransferase (ALT) Rattus norvegicus (Rat) Recombinant protein

GPT; ALAT; SGPT; AAT1; GPT1; Serum Glutamic Pyruvic Transaminase; Alanine Transaminase; Glutamate pyruvate transaminase 1; Glutamic--alanine transaminase 1

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Molecular Mass 28.3kDa, Accurate 27kDa(Analysis of differences refer to the manual)
  • Residues & TagsGln203~Leu443 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 97%
  • Isoelectric Point5.6
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  • Recombinant Alanine Aminotransferase (ALT) Packages (Simulation)
  • Recombinant Alanine Aminotransferase (ALT) Packages (Simulation)
  • RPA207Ra02.jpg SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Enhanced antitumor activity by combining an adenovirus harboring ING4 with cisplatin for hepatocarcinoma cellsNature: 201067
  • Additive effects of nicotine and high-fat diet on hepatic steatosis in male mice.PubMed: 23093702
  • Adrenocorticotropic hormone ameliorates acute kidney injury by steroidogenic-dependent and -independent mechanismsPubMed: PMC3612362
  • Pathogenesis of emerging severe fever with thrombocytopenia syndrome virus in C57/BL6 mouse modelPubMed: PMC3382536
  • Protective effect of Panax ginseng against N-acetyl-p-aminophenol-induced hepatotoxicity in ratsAcademicjournals: Source
  • Adrenocorticotropic hormone ameliorates acute kidney injury by steroidogenic-dependent and-independent mechanismsPubmed: 23325074
  • Protective effect of Et-1 receptor antagonist bosentan on paracetamol induced acute liver toxicity in ratsScienceDirect: S0014299914000235
  • Bifidobacterium pseudocatenulatum CECT7765 promotes a TLR2-dependent anti-inflammatory response in intestinal lymphocytes from mice with cirrhosisPubmed:25657013
  • Resolvin E1 and chemerin C15 peptide do not improve rodent non-alcoholic steatohepatitisPubmed:25681756
  • Assessing the Effect of Leptin on Liver Damage in Case of Hepatic Injury Associated with Paracetamol PoisoningPubMed: 26697061
  • The effects of subacute deltamethrin exposure on rat liver: histochemical, immunohistochemical, and biochemical studyxmlui:11446
  • Advanced Studies in Clinical and Experimental Research in GastroenterologyPubmed:26955389
  • Effects of developmental exposure to silver in ionic and nanoparticle form: A study in ratspubmed:27716437
  • Dihydromyricetin protects against liver ischemia/reperfusion induced apoptosis via activation ofFOXO3a-mediated autophagy.pubmed:27793014
  • Adiponectin protects the rats liver against chronic intermittent hypoxia induced injury throughAMP-activated protein kinase pathway.pubmed:27678302
  • Modulation of gut microbiota contributes to curcumin-mediated attenuation of hepatic steatosis in ratspubmed:28341485
  • Antifibrotic Effect of Lactulose on a Methotrexate-Induced Liver Injury Modelpubmed:28912805
  • Alcohol-associated intestinal dysbiosis impairs pulmonary host defense against Klebsiella pneumoniae.pubmed:28604843
  • A critical role of hepatitis B virus polymerase in cirrhosis, hepatocellular carcinoma, and steatosisPubmed:29321963
  • Rolle von CMKLR1 und dessen Liganden bei der nicht-alkoholischen FettlebererkrankungRegensburg:Source
  • The valuable effects of potent antioxidant curcumin in cisplatin induced liver and kidney injuryDOI:Source
  • Hepatoprotective activity of Erythrina× neillii leaf extract and characterization of its phytoconstituentsPubmed: 28095910
  • Brg1 promotes liver regeneration after partial hepatectomy via regulation of cell cyclePubmed: 30787318
  • Corticosterone-mediated microglia activation affects dendritic spine plasticity and motor learning functions in minimal hepatic encephalopathyPubmed: 31437533
  • Liraglutide Attenuates Nonalcoholic Fatty Liver Disease by Modulating Gut Microbiota in Rats Administered a High-Fat DietPubmed: 32149099
  • CD47 Deficiency in Mice Exacerbates Chronic Fatty Diet-Induced Steatohepatitis Through Its Role in Regulating Hepatic Inflammation and Lipid MetabolismPubmed: 32158445
  • Biological Safety and Biodistribution of Chitosan NanoparticlesPubmed: 32340313
  • Effects of Tempol in Lipopolysaccharide-Induced Liver Injury
  • Antimalarial Effect of the Total Glycosides of the Medicinal Plant, Ranunculus japonicus33925018
  • An Experimental Study: Benefits of Digoxin on Hepatotoxicity Induced by Methotrexate Treatment34804155
  • Taraxasterol mitigates Con A-induced hepatitis in mice by suppressing interleukin-2 expression and its signaling in T lymphocytes34848154
  • Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver …Pubmed:35212074
  • Blockade of mIL‐6R alleviated lipopolysaccharide‐induced systemic inflammatory response syndrome by suppressing NF‐κB‐mediated Ccl2 expression and …Pubmed:35548710
  • Dimethyl fumarate ameliorates autoimmune hepatitis in mice by blocking NLRP3 inflammasome activationPubmed:35605433

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