Recombinant Alkaline Phosphatase, Tissue-nonspecific (ALPL) 
BALP; BSAP; HOPS; AP-TNAP; TNSALP; Bone Alkaline Phosphatase; Alkaline Phosphatase,Tissue-Nonspecific Isozyme; Alkaline Phosphatase, Liver/Bone/Kidney
Overview
Properties
- Product No.RPB091Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
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Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
Research use only - DownloadInstruction Manual
- CategoryEnzyme & KinaseMetabolic pathwayHepatologyBone metabolism
- Source Prokaryotic expression, Host E.coli
- Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
- Subcellular LocationMembrane
- Molecular Mass 39.3kDa, Accurate 39kDa(Analysis of differences refer to the manual)
- Residues & TagsLeu18~Arg335 with N-terminal His Tag
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder, Purity > 95%
- Isoelectric Point7.0
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Packages (Simulation)
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Packages (Simulation)
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Figure . Gene Sequencing (extract)
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Figure. SDS-PAGE
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ISO9001: 2008, ISO13485: 2003 Registered
Sequence
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Increment services
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Endotoxin Removal Kit
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BCA Protein Quantification Kit
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Protein Labeling Customized Service
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Molecular Mass Marker for Protein
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Recombinant Protein Customized Service
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Monoclonal Antibody Customized Service
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Polyclonal Antibody Customized Service
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Protein Activity Test Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Buffer
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Endotoxin Removal Kit II
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Real Time PCR Experimental Service
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Spike RBD Protein (S-RBD)
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Protein G
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Protein A
Citations
- Osteopenic bone cell response to strontium-substituted hydroxyapatiteSpringerLink: n8233337w171v451
- Dextromethorphan inhibits osteoclast differentiation by suppressing RANKL-induced nuclear factor-κB activationPubMed: 23400250
- Chronic exposure to low concentrations of strontium 90 affects bone physiology but not the hematopoietic system in miceWiley: Source
- The antidepressant bupropion exerts alleviating properties in an ovariectomized osteoporotic rat modelPubmed:25544359
- Enhanced differentiation of osteoblastic cells on novel chitosan/β-1, 3-glucan/bioceramic scaffolds for bone tissue regenerationPubmed:25586067
- Effect of Mirtazapine on Rat Bone Tissue after OrchidectomyPubMed: 25871861
- New method for the fabrication of highly osteoconductive β-1,3-glucan/HA scaffold for bone tissue engineering: Structural, mechanical, and biological characterization.Pubmed:27239050
- MiR-142-5p promotes bone repair by maintaining osteoblast activityPubmed:27085967
- New method for the fabrication of highly osteoconductive β‐1, 3‐glucan/HA scaffold for bone tissue engineering: Structural, mechanical, and biological …doi:10.1002
- MiR‑142‑5p promotes bone repair by maintaining osteoblast activitypubmed:27085967
- Hydrolysis of Extracellular Pyrophosphate increases in post-hemodialysis plasmaPubmed:30038263
- Suppression Effect of Astaxanthin on Osteoclast Formation In Vitro and Bone Loss In VivoPubmed:29562730
- Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive RatsPubmed:29898457
- Combination therapy with BMP‑2 and psoralen enhances fracture healing in ovariectomized mice10.3892:etm.2018.6353
- Long non-coding RNA SNHG7 promotes the fracture repair through negative modulation of miR-9
- The effect of low temperature atmospheric nitrogen plasma on MC3T3-E1 preosteoblast proliferation and differentiation in vitro
- Bovine Hydroxyapatite-Based Bone Scaffold with Gentamicin Accelerates Vascularization and Remodeling of Bone Defect34104195