Recombinant Apoptosis Signal Regulating Kinase 1 (ASK1) Homo sapiens (Human) Recombinant protein

MAP3-K5; MAP3K5; ASK1; MAPKKK5; MEKK5; Mitogen-Activated Protein Kinase Kinase Kinase 5; MAPK/ERK kinase kinase 5

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationCytoplasm
  • Molecular Mass 32.2kDa, Accurate 32kDa(Analysis of differences refer to the manual)
  • Residues & TagsGly681~Glu936 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 97%
  • Isoelectric Point6.7
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  • Recombinant Apoptosis Signal Regulating Kinase 1 (ASK1) Packages (Simulation)
  • Recombinant Apoptosis Signal Regulating Kinase 1 (ASK1) Packages (Simulation)
  • RPB358Hu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Heat-shock protein 70 modulates apoptosis signal-regulating kinase 1 in stressed hepatocytes of Mugil cephalusPubmed:24875452
  • Differential reCavia (Guinea pig )lation of pro- and antiapoptotic proteins in fish adipocytes during hypoxic conditions.Pubmed:26744268
  • ASK-1 Expression Down-regulated in Preeclamptic Placental Explants Incubated with Mint-Tea:
  • Differential expression of survival proteins during decreased intracellular oxygen tension in brain endothelial cells of grey mullets.pubmed:28043644
  • Upregulation of HSP70 Extends Cytoprotection to Fish Brain under Xenobiotic Stressb0407ebde042c4f61b728bc11a586e6c

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