Recombinant Beta-Site APP Cleaving Enzyme 1 (bACE1)

ASP2; Beta Secretase; Memapsin 2; Aspartyl protease 2; Beta-site amyloid precursor protein cleaving enzyme 1; Membrane-associated aspartic protease 2

UOM
1. 10µg
2. 50µg
3. 200µg
4. 1mg
5. 5mg
FOB US$ 120.00 US$ 300.00 US$ 600.00 US$ 1,800.00 US$ 4,500.00
Quantity

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Overview

Properties

Product No.RPA718Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
Applications Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
Research use only
DownloadInstruction Manual
CategoryNeuro science

Source Prokaryotic expression, Host E.coli
Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
Subcellular LocationMembrane, Endoplasmic reticulum lumen
Molecular Mass 59.1kDa, Accurate 59kDa(Analysis of differences refer to the manual)
Residues & TagsPhe170~Thr428 with N-terminal His and GST Tag
Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits Freeze-dried powder, Purity > 90%
Isoelectric Point5.2

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Packages (Simulation)
Packages (Simulation)

Figure. Gene Sequencing (Extract)

SDS-PAGE

ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Polyacrylamide Gel Electrophoresis (PAGE) Experiment Service

Increment services

Citations

Comparative study of the effects of phosphatidylcholine rich in DHA and EPA on Alzheimer’s disease and the possible mechanisms in CHO-APP/PS1 cells and SAMP8 micepubmed:29292421
EPA enriched ethanolamine plasmalogens significantly improve cognition of Alzheimer's disease mouse model by suppressing β-amyloid generation10.1016/j.jff.2017.12.016
Mechanisms of DHA-enriched phospholipids in improving cognitive deficits in aged SAMP8 mice with high-fat dietPubmed:29986309
Involvement of hippocampal agmatine in β1-42 amyloid induced memory impairment, neuroinflammation and BDNF signaling disruption in micePubmed: 32522471
Effects of Alcohol Intake on Cognitive Function and ¦Â-amyloid Protein in APP/PS1 Transgenic Mice33737111