Recombinant Calreticulin (CALR) Rattus norvegicus (Rat) Recombinant protein

CRT; RO; SSA; CC1qR; ERp60; HACBP; grp60; CRTC; CRP55; Calregulin; Sicca Syndrome Antigen A; Autoantigen Ro; Endoplasmic reticulum resident protein 60

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted, Cytoplasm
  • Molecular Mass 32.7kDa, Accurate 38kDa(Analysis of differences refer to the manual)
  • Residues & TagsAla72~Lys322 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point4.5
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  • Recombinant Calreticulin (CALR) Packages (Simulation)
  • Recombinant Calreticulin (CALR) Packages (Simulation)
  • Recombinant Calreticulin (CALR) Figure . Gene Sequencing (extract)
  • RPB486Ra01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Oxidative Stress-Induced Calreticulin Expression and Translocation: New Insights into the Destruction of MelanocytesPubmed: 23771121
  • The Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKCMdpi: Source
  • Amniotic fluid calreticulin in pregnancies complicated by the preterm prelabor rupture of membranesPubmed:26953684
  • Cervical fluid calreticulin and cathepsin-G in pregnancies complicated by preterm prelabor rupture of membranes.pubmed:28152632
  • Investigation of the interaction between calreticulin and immunoglobulin G by capillary electrophoresis and computer modelingDoi: 10.1016/j.talanta.2018.11.098
  • Thermostable ionizable lipid-like nanoparticle (iLAND) for RNAi treatment of hyperlipidemiaPubmed:35171673

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